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Methyl jasmonate-induced cell death in grapevine requires both lipoxygenase activity and functional octadecanoid biosynthetic pathway

  • Section Botany
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Abstract

Grapevine (Vitis vinifera L., cv. Limberger) leaf tissues and suspension-cultured cells were induced to undergo programmed cell death (PCD) by exogenously added methyl jasmonate (MeJA). The elicitor signaling pathway involved in MeJA-induced PCD was further investigated using pharmacological, biochemical and histological approaches. Pharmacological dissection of the early events preceding the execution of MeJA-triggered PCD indicated that this process strongly depends on both, de novo protein and mRNA synthesis. Treatment of leaf discs and cell suspensions with lipase inhibitor Ebelactone B and specific lipoxygenase inhibitor Phenidone blocked MeJA-induced PCD. These results suggest that some chloroplast membrane-derived compound(s) is required for MeJA-induced PCD in grapevine. The progression of MeJAtriggered PCD may be further inhibited by the use of metabolic inhibitors of key enzymes of octadecanoid biosynthesis including AOS, AOC, and OPR indicating that the functional jasmonate biosynthetic pathway is an integral part of the MeJA-induced signal transduction cascade that results in the coordinate expression of events leading to PCD. Finally, the activation of the octadecanoid pathway, as a critical point in MeJA-induced PCD, was independently demonstrated with cellulysin, a macromolecular elicitor acting via octadecanoid signaling. The cellulysin was shown to be a very potent enhancer of MeJA-triggered PCD in grapevine cells.

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Abbreviations

AOC:

allene oxide cyclase

AOS:

allene oxide synthase

CHX:

cycloheximide

COR:

cordycepin

DIECA:

diethyldithiocarbamic acid

HR:

hypersensitive response

JA:

jasmonic acid

LOX:

lipoxygenase

MeJA:

methyljasmonate

NIA:

necrosis-inducing activity

OPDA:

12-oxophytodienoic acid

OPR:

12-oxophytodienoic acid reductase

qRT-PCR:

quantitative real-time polymerase chain reaction

TMV:

tobacco mosaic virus

TUB:

tubulin

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Authors and Affiliations

  1. Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84523, Bratislava, Slovakia

    Vladimír Repka, Mária Čarná & Ján Pavlovkin

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  1. Vladimír Repka
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  2. Mária Čarná
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  3. Ján Pavlovkin
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Correspondence to Vladimír Repka.

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Repka, V., Čarná, M. & Pavlovkin, J. Methyl jasmonate-induced cell death in grapevine requires both lipoxygenase activity and functional octadecanoid biosynthetic pathway. Biologia 68, 896–903 (2013). https://doi.org/10.2478/s11756-013-0220-4

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