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Protection against experimental salmonellosis by recombinant 49 kDa OMP of Salmonella enterica serovar Typhi: biochemical aspects

  • Section Cellular and Molecular Biology
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Abstract

Typhoid fever is systemic illness caused by Salmonella enterica serovar Typhi (S. Typhi) in humans. Increasing multidrug resistant strains of S. Typhi and limited effect of available vaccines has necessitated exploring of new immunogens for protection against it. Earlier studies have shown that a crude preparation of outer membrane proteins (OMPs) of S. Typhi evokes strong immune response and induces a protective immunity against infection caused by diverse Gram-negative bacteria. In the present study we have evaluated the protective effect of a purified recombinant 49 kDa (r49kDa) OMP of S. Typhi alone or along with alum or complete Freund’s adjuvant, against a challenge by S. Typhi (0.4 × 50% lethal dose) by biochemical estimation of serum enzymes and oxidative stress enzymes in Swiss albino mice. There was a decrease in activity of alanine aminotransferase by 14.28%, 38.09%, 23.80%; aspartate aminotransferase by 6.25%, 25%, 16.25%; lipid peroxidation by 4.34%, 18.84%, 11.59%; and catalase by 8%, 14%, 10%, respectively, whereas increase in activity of reduced glutathione by 33.33%, 61.11%, 44.44%; glutathione peroxidase by 7%, 16%, 10%; and glutathione reductase by 8%, 20%, 12%, respectively, as compared to control animals challenged with bacteria without pre-immunization. The results indicated that immunization of mice with r49kDa OMP alone or in combination with adjuvants protected and normalized the liver. It reduces the development of oxidative stress in mice against Salmonella infection and the risk of getting typhoid. These results represent an additional supplement to our earlier reported data on protective immunity evoked by this protein.

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Abbreviations

ALT:

alanine aminotransferase

AST:

aspartate aminotransferase

CAT:

catalase

CFA:

complete Freund’s adjuvant

GPX:

glutathione peroxidase

GR:

glutathione reductase

GSH:

reduced glutathione

IEC:

intestinal epithelial cell

IFA:

incomplete Freund’s adjuvant

LD50:

lethal dose 50%

LPO:

lipid peroxidation

MDA:

malondialdehyde

MDR:

multidrug-resistant

OMP:

outer membrane protein

PMS:

post mitochondrial supernatant

r49kDa:

recombinant 49 kDa

S. Typhi:

Salmonella enterica serovar Typhi

TCA:

trichloroacetic acid

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  1. Department of Biotechnology, Hamdard University, Hamdard Nagar, New Delhi, 110062, India

    Jasmine Kaur & Swatantra K. Jain

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  1. Jasmine Kaur
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  2. Swatantra K. Jain
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Correspondence to Swatantra K. Jain.

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Kaur, J., Jain, S.K. Protection against experimental salmonellosis by recombinant 49 kDa OMP of Salmonella enterica serovar Typhi: biochemical aspects. Biologia 68, 194–201 (2013). https://doi.org/10.2478/s11756-013-0160-z

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