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Molecular cloning and expression analysis of a new WD40 repeat protein gene in upland cotton

  • Section Botany
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Abstract

A new member of the WD repeat protein family, named GhWD40, was cloned from a near-isogenic line for glands in cotton. It has 2629 bp cDNA and a complete opening reading frame (ORF) of 1239 bp, containing the initial code (ATG) and terminal code (TAG); there is a 1061 bp non-coding sequence at the 5′-end, and a 329 bp non-coding sequence at the 3′-end, including the poly(A) sequence (accession number: JN714279). The predicted protein of the complete ORF comprised 412 amino acids with a calculated molecular mass of 47.1 kDa and an isoelectric point of 8.88. Protein domain scanning showed that the novel protein has five wd40 motifs and belongs to the WD40 family. From a search for GhWD40 cDNA and amino acid sequences in the database, it has 77% sequence identity and was 90% sequence positive with the WD-40 repeat protein from Trifolium pratense (accession number BAE71307.1), and 80% sequence identity and 89% sequence positivity with the ribosome biogenesis protein bop1 from Ricinus communis (accession number XP 002529002.1). We propose that GhWD40 may play the same role as bop1. In addition, expression of GhWD40 in near-isogenic lines 11 and 3 (with and without glands, respectively) was studied by quantitative RT-polymerase chain reaction, and the level in near-isogenic line 11 was higher than that in near-isogenic line 3, suggesting that GhWD40 may be related to gland formation.

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Abbreviations

ORF:

opening reading frame

ATG:

initial code

TAG:

terminal code

RACE:

rapid amplification of cDNA ends

CAP:

Contig Assembly Program

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Authors and Affiliations

  1. College of Bioinformation, Chongqing University of Posts and Telecommunications, Chongqing, 400065, China

    Quan Sun, Yingfan Cai, Xiaohong He & Huaizhong Jiang

  2. Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Southwest University, Chongqing, 400716, China

    Xiaoyan Zhu & Guanghua He

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  1. Quan Sun
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  2. Yingfan Cai
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  3. Xiaoyan Zhu
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  4. Xiaohong He
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  5. Huaizhong Jiang
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  6. Guanghua He
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Correspondence to Yingfan Cai.

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Sun, Q., Cai, Y., Zhu, X. et al. Molecular cloning and expression analysis of a new WD40 repeat protein gene in upland cotton. Biologia 67, 1112–1118 (2012). https://doi.org/10.2478/s11756-012-0103-0

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  • DOI: https://doi.org/10.2478/s11756-012-0103-0

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