Biologia

, Volume 67, Issue 4, pp 777–787 | Cite as

Population genetic structure of Simulium degrangei (Diptera: Simuliidae) from Western Carpathians

Full Paper Section Cellular and Molecular Biology
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Abstract

Simulium degrangei Dorier & Grenier, 1960 was recorded in southern and central Europe and in the Crimea and Caucasus. Its distribution pattern is scattered. The variability of the mtDNA gene encoding cytochrome oxidase I was studied in populations of S. degrangei from the Western Carpathians in Slovakia and in the northern Hellenides of Greece. In the analyzed samples, 21 haplotypes were recorded, of which twenty were private and occurred in only one mountain range, but one haplotype was found in all three studied populations from the Western Carpathians. Both haplotypes from the Hellenides were private, but these were not isolated and they fit into the haplotype network of the Western Carpathians. Statistics of genetic variability, different designs of analysis of molecular variation with non-significant differences and the pair-wise genetic distances support the hypothesis that there are no differences between the analyzed populations. Similarly, using nested clade phylogeographical analysis, no genetic-geographical structure was found.

Key words

Simulium degrangei Dorier & Grenier cytochrome oxidase I Western Carpathians Hellenides genetic variability 

Abbreviations

COI

cytochrome oxidase I

HT

haplotype

NCPA

nested clade phylogeographical analysis

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References

  1. Adler P.H., Cheke R.A. & Post R.J. 2010. Evolution, epidemiology, and population genetics of black flies (Diptera: Simuliidae). Infect. Genetics Evol. 10: 846–865.CrossRefGoogle Scholar
  2. Adler P.H. & Crosskey R.W. 2011. World Blackflies (Diptera: Simuliidae): A Comprehensive Revision of the Taxonomic and Geographical Inventory [2011]. http://entweb.clemson.edu/biomia/pdfs/blackflyinventory.pdf (accessed 05 SEP 2011).
  3. Adler P.H. & McCreadie J.W. 1997. The hidden ecology of black flies: sibling species and ecological scale. Amer. Entomologist 43: 153–161.Google Scholar
  4. Ball S.L., Hebert P.D.N., Burian S.K. & Webb J.M. 2005. Biological indentifications of mayflies (Ephemeroptera) using DNA barcodes. J. North Amer. Benthol. Soc. 24: 508–524.Google Scholar
  5. Barrett R.D.H. & Hebert P.D.N. 2005. Identifying spiders through DNA barcodes. Can. J. Zool. 83: 481–491.CrossRefGoogle Scholar
  6. Brown B., Emberson R.M. & Paterson A.M. 1999. Mitochondrial COI and II provide useful markers for Weiseana (Lepidoptera, Hepialidae) species identification. Bull. Entomol. Res. 89: 287–294.CrossRefGoogle Scholar
  7. Bucklin A., Guarnieri M., Hill R.S., Bentley A.M. & Kaartvedt S. 1999. Taxonomic and systematic assessment of planktonic copepods using mitochondrial COI sequence variation and competitive, species-specific PCR. Hydrobiology 401: 239–254.CrossRefGoogle Scholar
  8. Carew M.E., Pettigrove V., Cox R.L. & Hoffmann A.A. 2007. DNA identification of urban Tanytarsini chironomids (Diptera: Chironomidae). J. North Amer. Benthol. Soc. 26: 587–600.CrossRefGoogle Scholar
  9. Clement M., Posada D. & Crandall K.A. 2000. TCS: a computer program to estimate gene genealogies. Mol. Ecol. 9: 1657–1659.PubMedCrossRefGoogle Scholar
  10. Conflitti I.M., Kratochvil M.J., Spironello M., Shields G.F. & Currie D.C. 2010. Good species behaving badly: nonmonophyly of black fly sibling species in the Simulium arcticum complex (Diptera: Simuliidae). Mol. Phylogen. Evol. 57: 245–257.CrossRefGoogle Scholar
  11. Crandall K.A. 1996. Multiple interspecies transmissions of human and simian T-cell leukemia/lymphoma virus type I sequences. Mol. Biol. Evol. 13: 115–131.PubMedCrossRefGoogle Scholar
  12. Crandall K.A. & Templeton A.R. 1993. Empirical tests of some predictions from coalescent theory with applications to intraspecific phylogeny reconstruction. Genetics 134: 959–969.PubMedGoogle Scholar
  13. Crosskey R.W. & Zwick H. 2007. New faunal records, with taxonomic annotations, for the blackflies of Turkey (Diptera, Simuliidae). Aquatic Insects 29: 21–48.CrossRefGoogle Scholar
  14. Cywinska A., Hunter F.F. & Hebert P.D.N. 2006. Identifying Canadian mosquito species through DNA barcodes. Med. Vet. Entomol. 20: 413–424.PubMedCrossRefGoogle Scholar
  15. Day J.C., Goodall T.I. & Post R.J. 2008. Confirmation of the species status of the blackfly Simulium galeratum in Britain using molecular taxonomy. Med. Vet. Entomol. 22: 55–61.PubMedCrossRefGoogle Scholar
  16. Day J.C., Mustapha M. & Post R.J. 2010. The subgenus Eusimulium (Diptera: Simuliidae: Simulium) in Britain. Aquatic Insects 32: 281–292.CrossRefGoogle Scholar
  17. Dinulescu G. 1966. Fauna Republicii Socialiste Romania. Insecta, Diptera, Fam. Simuliidae. Editura Academiei Republicii Socialiste Romania, Bucuresti, 604 pp.Google Scholar
  18. Duncan G.A., Adler P.H., Pruess K.P. & Powers T.O. 2004. Molecular differentiation of two sibling species of the black fly Simulium vittatum (Diptera: Simuliidae) based on random amplified polymorphic DNA. Genome 47: 373–379.PubMedCrossRefGoogle Scholar
  19. Dušinský R., Kúdela M., Stloukalová V. & Jedlička L. 2006. Use of inter-simple sequence repeat (ISSR) markers for discrimination between and within species of blackflies (Diptera: Simuliidae). Biologia 61: 299–304.CrossRefGoogle Scholar
  20. Excoffier L., Laval G. & Schneider S. 2005. Arlequin ver.3.0: an integrated software package for population genetics data analysis. Evol. Bioinform. Online 1: 47–50.Google Scholar
  21. Finn D.S. & Adler P.H. 2006. Population genetic structure of a rare high-elevation black fly, Metacnephia coloradensis, occupying Colorado lake outlet streams. Freshwater Biol. 51: 2240–2251.CrossRefGoogle Scholar
  22. Finn D.S., Theobald D.M., Black W.C. & Poff N.L. 2006. Spatial population genetic structure and limited dispersal in a Rocky Mountain alpine stream insect. Mol. Ecol. 15: 3553–3566.PubMedCrossRefGoogle Scholar
  23. Folmer O., Black M., Hoeh W., Lutz R. & Vrijenhoek R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol. Mar. Biol. Biotechnol. 3: 294–299.PubMedGoogle Scholar
  24. Hebert P.D.N., Cywinska A., Ball S.L. & de Waard J.R. 2003. Biological identifications through DNA barcodes. Proc. R. Soc. B — Biol. Sci. 270: 313–321.CrossRefGoogle Scholar
  25. Ilmonen J., Adler P.H., Malmqvist B. & Cywinska A. 2009. The Simulium vernum group (Diptera: Simuliidae) in Europe: multiple character sets for assessing species status. Zool. J. Linn. Soc. 156: 847–863.CrossRefGoogle Scholar
  26. Jedlička L. 2006. Distribution of three high altitude black fly species (Diptera: Simuliidae). Studia Dipterol. Suppl. 14: 45–59.Google Scholar
  27. Jedlička L., Kúdela M. & Stloukalová, V., 2004: Key to the identification of blackfly pupae (Diptera: Simuliidae) of Central Europe. Biologia 59(Suppl. 15): 157–178.Google Scholar
  28. Kovachev S. 1979. Saprobni sekvencii pri semeystvo Simuliidae i znachenieto im za opredeliane kachestvoto na vodata. Hydrobiology 9: 50–56.Google Scholar
  29. Kúdela M. 2011: New records of Simulium degrangei (Diptera: Simuliidae) in Slovakia. Folia Faunistica Slovaca 16: 181–183.Google Scholar
  30. Librado P. & Rozas J. 2009: DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25: 1451–1452.PubMedCrossRefGoogle Scholar
  31. Malicky H. 1983. Chorological patterns and biome types of European Trichoptera and other freshwater insects. Arch. Hydrobiol. 96: 223–244.Google Scholar
  32. Malicky H. 2000. Arealdynamik und Biomgrundtypen am Beispiel der Köcherfliegen (Trichoptera). Entomol. Basil. 22: 235–259.Google Scholar
  33. Morales-Hojas R. & Krueger A. 2009. The species delimitation problem in the Simulium damnosum complex, blackfly vectors of onchocerciasis. Med. Vet. Entomol. 23: 257–268.PubMedCrossRefGoogle Scholar
  34. Morales-Hojas R., Post R.J., Cheke R.A. & Wilson M.D. 2002a. Assessment of rDNA IGS as a molecular marker in the Simulium damnosum complex. Med. Vet. Entomol. 16: 395–403.PubMedCrossRefGoogle Scholar
  35. Morales-Hojas R., Post R.J., Wilson M.D. & Cheke R.A. 2002b. Completion of the sequence of the nuclear ribosomal DNA subunit of Simulium sanctipauli, with descriptions of the 18S, 28S genes and the IGS. Med. Vet. Entomol. 16: 386–394.PubMedCrossRefGoogle Scholar
  36. Novák V.J.A. 1956. Příspěvek k poznání muchniček (Simuliidae, Diptera) ČSR. Věstník čsl. zool. spol. 20: 224–248.Google Scholar
  37. Panchal M. & Beaumont M.A. 2007. The automation and evaluationof nested clade phylogeographic analysis. Evolution 61: 1466–1480.PubMedCrossRefGoogle Scholar
  38. Pauls S.U., Lumbsch H.T. & Haase P. 2006. Phylogeography of the montane cadddisfly Drusus discolor: evidence for multiple refugia and periglacial survival. Mol. Ecol. 15: 2153–2169.PubMedCrossRefGoogle Scholar
  39. Posada D. & Crandall K.A. 2001. Intraspecific gene genealogies: trees grafting into networks. Trends Ecol. Evol. 16: 37–45.PubMedCrossRefGoogle Scholar
  40. Posada D., Crandall K.A. & Templeton A.R. 2000. GeoDis: a program for the cladistic nested analysis of the geographical distribution of genetic haplotypes. Mol. Ecol. 9: 487–488.PubMedCrossRefGoogle Scholar
  41. Posada D., Crandall K.A. & Templeton A.R. 2006. Nested clade analysis statistics. Mol. Ecol. Notes 6: 590–593.CrossRefGoogle Scholar
  42. Post R.J., Flook P.K., Millest A.L., Cheke R.A., McCall P.J., Wilson M.D., Mustapha M., Somiari S., Davies J.B., Mank R.A., Geenen P., Enyong P., Sima A. & Mas J. 2003. Cytotaxonomy, morphology and molecular systematics of the Bioko form of Simulium yahense (Diptera: Simuliidae). Bull. Entomol. Res. 93: 145–157.PubMedCrossRefGoogle Scholar
  43. Pramual P., Kuvangkadilok C., Baimai V. & Walton C. 2005. Phylogeography of the black fly Simulium tani (Diptera: Simuliidae) from Thailand as inferred from mtDNA sequences. Mol. Ecol. 14: 3989–4001.PubMedCrossRefGoogle Scholar
  44. Rivera J. & Currie D.C. 2009. Identification of Nearctic black flies using DNA barcodes (Diptera: Simuliidae). Mol. Ecol. Res. 9: 224–236.CrossRefGoogle Scholar
  45. Schmitt T., Muster C. & Schönswetter P. 2010. Are Disjunct Alpine and Arctic-Alpine Animal and Plant Species in the Western Palearctic Really “Relics of a Cold Past”?, pp. 239–252. In: Habel J.C. & Assmann T. (eds), Relict Species: Phylogeography and Conservation Biology, Springer-Verlag, Berlin, Heidelberg.Google Scholar
  46. Stloukalová V. & Jedlička L. 2005. Distribution of blackflies (Diptera: Simuliidae) in geomorphological units in Slovakia. Entomofauna Carpathica 17: 86–110.Google Scholar
  47. Tamura K. & Nei M. 1993: Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol. Biol. Evol. 10: 512–526.PubMedGoogle Scholar
  48. Tamura K., Peterson D., Peterson N., Stecher G., Nei M. & Kumar S. 2011: MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28: 2731–2739.PubMedCrossRefGoogle Scholar
  49. Tang J., Toe L., Back C., Zimmerman P.A., Pruess K. & Unnasch T.R. 1995. The Simulium damnosum species complex: phylogenetic analysis and molecular identification based upon mitochondrially encoded gene sequences. Insect Mol. Biol. 4: 79–88.PubMedCrossRefGoogle Scholar
  50. Templeton A.R. 1998. Nested clade analyses of phylogeographic data: testing hypotheses about gene flow and population history. Mol. Ecol. 7: 381–397.PubMedCrossRefGoogle Scholar
  51. Templeton A.R. 2001. Using phylogeographic analyses of gene trees to test species status and processes. Mol. Ecol. 10: 779–791.PubMedCrossRefGoogle Scholar
  52. Templeton A.R. 2004. Statistical phylogeography: methods of evaluating and minimizing inference errors. Mol. Ecol. 13: 789–809.PubMedCrossRefGoogle Scholar
  53. Templeton A.R. 2005. Haplotype trees and modern human origins. Am. J. Phys. Anthropol. Suppl. 41: 33–59.CrossRefGoogle Scholar
  54. Templeton A.R., Boerwinkle E. & Sing C.F. 1987. A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonu-clease mapping1. Basic theory and an analysis of alcohol dehydrogenase activity in Drosophila. Genetics 117: 343–351.PubMedGoogle Scholar
  55. Templeton A.R., Crandall K.A. & Sing C.F. 1992. A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping and DNA sequence data 3. Cladogram estimation. Genetics 132: 619–633.Google Scholar
  56. Templeton A.R., Routman E. & Phillips C.A. 1995. Separating population structure from population history: a cladistic analysis of the geographical distribution of mitochondrial DNA haplotypes in the tiger salamander, Ambystoma tigrinum. Genetics 140: 767–782.PubMedGoogle Scholar
  57. Templeton A.R. & Sing C.F. 1993. A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping. IV. Nested analyses with cladogram uncertainty and recombination. Genetics 134: 659–669.PubMedGoogle Scholar
  58. Thanwisai A., Kuvangkadilok C. & Baimai V. 2006. Molecular phylogeny of black flies (Diptera: Simuliidae) from Thailand, using ITS2 rDNA. Genetica 128: 177–204.PubMedCrossRefGoogle Scholar
  59. Vincent S., Vian J.M. & Carlotti M.P. 2000. Partial sequencing of the cytochrome oxidase-b subunit gene. I. A tool for the identification of European species of blow flies for post mortem interval estimation. J. Forensic Sci. 45: 820–823.PubMedGoogle Scholar
  60. Xiong B. & Kocher T.D. 1991. Comparison of mitochondrial DNA sequences of seven morphospecies of black flies (Diptera: Simuliidae). Genome 34: 306–311.PubMedCrossRefGoogle Scholar
  61. Yankovsky A.V. 2003 [2002]: Opredeliteľ Moshek (Diptera: Simuliidae) Rossii i Sopredeñykh Territorii (Byvshego SSSR). Zoologicheski Institut RAN, Sankt-Peterburg, 570 pp.Google Scholar
  62. Zhu X., Pruess K.P. & Powers T.O. 1998: Mitochondrial DNA polymorphism in a black fly, Simulium vittatum (Diptera: Simuliidae). Can. J. Zool. 76: 440–447.Google Scholar
  63. Zimmerman P.A., Shapiro M., Tang J., Nutman T.B. & Unnasch T.R. 1995. Optimizing probe selection in directed heteroduplex analysis using HDProbe 1.1. Biotechniques 19: 972–977.PubMedGoogle Scholar
  64. Zivkovitch V. & Grenier P. 1959. Description d’une simulie nouvelle (S. ibariense n. sp.) (Diptera, Simuliidae) trouvee en Yougoslavie. Bull. Soc. Pathol. Exotique 52: 215–223.Google Scholar

Copyright information

© Versita Warsaw and Springer-Verlag Wien 2012

Authors and Affiliations

  1. 1.Department of Zoology, Faculty of Natural SciencesComenius UniversityBratislavaSlovakia
  2. 2.Department of Molecular Biology, Faculty of Natural SciencesComenius UniversityBratislavaSlovakia
  3. 3.Institute of Molecular BioMedicine, Faculty of Natural SciencesComenius UniversityBratislavaSlovakia

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