Skip to main content
SpringerLink
Log in

The effects of elicitation on the expression of key enzyme genes and on production of tropane alkaloids in Anisodus acutangulus plant

  • Section Cellular and Molecular Biology
  • Published:
Biologia Aims and scope Submit manuscript

Abstract

Tropane alkaloids (TAs) including hyoscyamine, anisodamine, anisodine and scopolamine are widely used as anticholinergic agents, which act on the parasympathetic nervous system, and their clinical demands are increasing rapidly. Anisodus acutangulus is a solanaceous perennial plant that is endemic to China with good potential for TA production and classified as an endangered species in China. Therefore there is a need to improve the TA production in this resource plant by biology methods. In our research, three different important elicitors including methyl jasmonate (MJ), yeast elicitor (YE) and abscisic acid (ABA) were used to study their effects on TA production, and genes expressions in A. acutangulus plant under each treatment were examined. The results revealed that TA production was induced by all the three treatments. However, its quantity varies with inducers used. The effect induced by MJ and YE were significant, while the effect induced by ABA was not obviously. To our knowledge, this is the first report on the relationship between TA production and genes expression profile induced by different elicitors in cultivated A. acutangulus plant, which should provide some useful information on how to improve TA production with the cultivated A. acutangulus plant in the near future.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

TA:

tropane alkaloid

ABA:

abscisic acid

DW:

dry weight

H6H:

hyoscyamine-6β-hydroxylase

MJ:

methyl jasmonate

PCR:

polymerase chain reaction

PMT:

putrescine N-methyltransferase

TR I:

tropinone reductase I

TR II:

tropinone reductase II

YE:

yeast extract

References

  • Arias Zabala M., Angarita M., Restrepo J.M., Caicedo L.A. & Perea M. 2010. Elicitation with methyl-jasmonate stimulates peruvoside production in cell suspension cultures of Thevetia peruviana. In Vitro Cell Dev. Biol. Plant. 46: 233–238.

    Article  Google Scholar 

  • Boitel-Conti M., Laberche J-C., Lanoue A., Ducrocq C. & Sangwan-Norreel B.S. 2000. Influence of feeding precursors on tropane alkaloid production during an abiotic stress in Datura innoxia transformed roots. Plant Cell Tiss. Org. 60: 131–137.

    Article  CAS  Google Scholar 

  • Dong H.D. & Zhong J.J. 2002. Enhanced taxane productivity in bioreactor cultivation of Taxus chinensis cells by combining elicitation, sucrose and ethylene incorporation. Enzyme Microb. Technol. 31: 116–121.

    Article  CAS  Google Scholar 

  • Ertola R.J. & Hours R.A. 1998. Role of yeast extract components in microbial cultures not associated with amino acids, vitamins and minerals: a review. Appl. Biol. Sci. 4: 1–15.

    CAS  Google Scholar 

  • Hasanloo T., Sepehrifar R., Rahnama H. & Shams M.R. 2009. Evaluation of the yeast-extract signaling pathway leading to silymarin biosynthesis in milk thistle hairy root culture. World J. Microbiol. Biotechnol. 25: 1901–1909.

    Article  CAS  Google Scholar 

  • Hattori T., Terada T. & Hamasuna S. 1995. Regulation of the Osem gene by abscisic acid and the transcriptional activator VP1: analysis of cis-acting promoter elements required for regulation by abscisic acid and VP1. Plant J. 7: 913–925.

    Article  PubMed  CAS  Google Scholar 

  • Ignatov A., Clark W.G., Cline S.D., Psenak M., Krueger J. & Coscia C.J. 1996. Elicitation of dihydrobenzophenanthridine oxidase in Sanguinaria canadiensis cell cultures. Phytochemistry 43: 1141–1144.

    Article  PubMed  CAS  Google Scholar 

  • Kai G.Y., Chen J. F., Li L., Zhou G.Y., Zhou L.M., Zhang L., Chen Y.H. & Zhao L.X. 2007. Molecular cloning and characterization of a new cDNA encoding hyoscyamine 6β-hydroxylase from roots of Anisodus acutangulus. J. Biochem. Mol. Biol. 40: 715–722.

    Article  PubMed  CAS  Google Scholar 

  • Kai G.Y., Li L., Wang J., Zhang Y., Zhang R., Yan X.M., Liao P., Lv X., Wang W., Lu Y. & Zhou G.Y. 2009a. Molecular cloning, characterization of two tropinone reductases in Anisodus acutangulus and enhancement of tropane alkaloids production in AaTRI-transformed hairy roots. Biotechnol. Appl. Biochem. 54: 177–186.

    Article  PubMed  CAS  Google Scholar 

  • Kai G.Y., Yang S., Luo X.Q., Zhou W.T., Fu X.Q., Zhang Y. & Xiao J.B. 2011. Co-expression of AaPMT and AaTRI effectively enhances tropane alkaloids production in Anisodus acutangulus hairy roots. BMC Biotechnol. 11: 43.

    Article  PubMed  CAS  Google Scholar 

  • Kai G.Y., Yang S., Zhang Y., Luo X.Q., Fu X.Q., Zhang A. & Xiao J.B. 2012. Effects of different elicitors on yield of tropane alkaloids in hairy roots of Anisodus acutangulus. Mol. Biol. Rep. 39: 1721–1729.

    Article  PubMed  CAS  Google Scholar 

  • Kai G.Y., Zhang Y., Chen J.F., Li L., Yan X.M., Zhang R., Liao P., Lv X., Wang W. & Zhou G.Y. 2009b. Molecular characterization and expression analysis of two distinct putrescine Nmethyltransferases from roots of Anisodus acutangulus. Physiol. Plant. 135: 121–129.

    Article  PubMed  CAS  Google Scholar 

  • Kang S.M. & Jung H.Y. 2004. Effects of methyl jasmonate and salicylic acid on the production of tropane alkaloids and the expression of PMT and H6H in adventitious root cultures of Scopolia parviflora. Plant Sci. 166: 745–751.

    Article  CAS  Google Scholar 

  • Kittipongpatana N., Davis D.L. & Porter J.R. 2002. Methyl jasmonate increases the production of valepotriates by transformed root cultures of Valerianella locusta. Plant Cell Tiss. Org. 71: 65–75.

    Article  CAS  Google Scholar 

  • Kreps J.A., Wu Y., Chang H.S., Zhu T., Wang X. & Harper J.F. 2002. Transcriptome changes for Arabidopsis in response to salt, osmotic, and cold stress. Plant Physiol. 130: 2129–2141.

    Article  PubMed  CAS  Google Scholar 

  • Lee K.T., Yamakawa T., Kodama T. & Shimomura K. 1998. Effect of chemicals on alkaloid production by belladonna transformed roots. Phytochem. 49: 2343–2347.

    Article  CAS  Google Scholar 

  • Li L., Wang J., Wang W., Lu Y., Wang Y.L., Zhou G.Y. & Kai G.Y. 2008. Optimization of induction and culture conditions and tropane alkaloid production in hairy roots of Anisodus acutangulus. Biotechnol. Bioprocess Eng. 13: 606–612.

    Article  CAS  Google Scholar 

  • Mundy J., Yamaguchi-Shinozaki K. & Chua N.H. 1990. Nuclear proteins bind conserved elements in the abscisic acidresponsive promoter of a rice rab gene. Proc. Natl. Acad. Sci. USA 87: 1406–1410.

    Article  PubMed  CAS  Google Scholar 

  • Narusaka Y., Nakashima K., Shinwari Z.K., Sakuma Y., Furihata T., Aabe H., Narusaka M., Shinozaki K. & Yamaguchi-Shinozaki K. 2003. Interaction between two cis-acting elements, ABRE and DRE, in ABA-dependent expression of Arabidopsis rd29A gene in response to dehydration and highsalinity stresses. Plant J. 34: 137–148.

    Article  PubMed  CAS  Google Scholar 

  • Pitta-Alvarez S.I., Spollansky T.C. & Giulietti A.M. 2000. The influence of different biotic and abiotic elicitors on the production and profile of tropane alkaloids in hairy root cultures of Brugmansia candida. Enzyme Microb. Technol. 26: 252–258.

    Article  PubMed  CAS  Google Scholar 

  • Rijhwani S.K. & Shaks J.V. 1998. Effect of elicitor dosage and exposure time on bisynthesis of indole alkaloids by Catharanthus roseus hairy root cultures. Biotechnol. Prog. 14: 442–449.

    Article  PubMed  CAS  Google Scholar 

  • Saedler R. & Baldwin I.T. 2003. Virus-induced gene silencing of jasmonate-induced direct defences, nicotine and trypsin proteinase-inhibitors in Nicotiana attenuate. J. Exp. Bot. 55: 151–157.

    Article  PubMed  Google Scholar 

  • Seung H.S. & Sang Y. B. 1998. Elicitation of camptothecin production in cell cultures of Camptotheca acuminate. Biotechnol. BioprocessEng. 3: 91–95.

    Article  Google Scholar 

  • Shams-Ardakani M.R., Hemmati S.H. & Mohagheghzadeh A.A. A. 2005. Effect of elicitors on the enhancement of podophylotoyin biosynthesis in suspension cultures of Linum album. Planta 13: 56–60.

    CAS  Google Scholar 

  • Shen Q. & Ho T.H.D. 1997. Promoter switches specific for abscisic acid (ABA)-induced gene expression in cereals. Physiol. Plant. 101: 653–664.

    Article  CAS  Google Scholar 

  • Shen Q., Zhang P. & Ho T.H.D. 1996. Modular nature of abscisic acid (ABA) response complexes: composite promoter units that are necessary and sufficient for ABA induction of gene expression in barley. Plant Cell 8: 1107–1119.

    Article  PubMed  CAS  Google Scholar 

  • Vasconsuelo A. & Boland R. 2007. Molecular aspects of the early stages of elicitation of secondary metabolites in plants. Plant Sci. 172: 861–875.

    Article  CAS  Google Scholar 

  • Wang C., Wu J. & Mei X. 2001. Enhancement of taxol production and excretion in Taxus chinensis cell culture by fungal elicitation and medium renewal. Appl. Microbiol. Biotechnol. 55: 404–410.

    Article  PubMed  CAS  Google Scholar 

  • Wang W. & Zhong J.J. 2002. Manipulation of ginsenoside heterogeneity in cell cultures of Panax notoginseng by addition of jasmonates. J. Biosci. Bioeng. 93: 48–53.

    PubMed  CAS  Google Scholar 

  • Wu D.K., Wang F.L., Chen Z.R., Yang J.S. & Huang Q.L. 1962. Chemical analysis of Anisodus acutangulus in Yunnan and extraction of atropine sulphate. Med. Pharm. Yunnan 3: 67–68.

    Google Scholar 

  • Xu C.M., Zhao B., Ou Y., Wang X.D., Yuan X.F. & Wang Y.C. 2007. Elicitor enhanced syringin production in suspension cultures of Sussurea medusa. World J. Microbiol. Biotechnol. 23: 965–970.

    Article  CAS  Google Scholar 

  • Xu D., Duan X., Wang B., Hong B., Ho. T.H.D. & Wu R. 1996. Expression of a late embryogenesis abundant protein gene, HVA1, from barley confers tolerance to water deficit and salt stress in transgenic rice. Plant Physiol. 110: 249–257.

    PubMed  CAS  Google Scholar 

  • Zarate R. & Verpoorte R. 2007. Strategies for the genetic modification of the medicinal plant Catharanthus roseus (L.) G. Don. Phytochem. Rev. 6: 475–491.

    Article  CAS  Google Scholar 

  • Zhang L., Ding R.X., Chai Y.R., Bonfill M., Pínol M.T., Xu T.F., Pi, Y., Wang Z.N., Zhang H.M., Kai G.Y., Liao Z.H., Sun X.F. & Tang K.X. 2004. Engineering tropane biosynthetic pathway in Hyoscyamus niger hairy root cultures. Proc. Natl. Acad. Sci. USA 101: 6786–6791.

    Article  PubMed  CAS  Google Scholar 

  • Zhao J., Davis L.C. & Verpoorte R. 2005. Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol. Adv. 23: 283–333.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234, People’s Republic of China

    Xiuqin Luo, Xueqing Fu, Jianbo Xiao & Guoyin Kai

  2. Department of Gynaecology and Obstetrics, Kunshan Hospital of Traditional Chinese Medicine, Kunshan, Jiangsu Province, 215300, People’s Republic of China

    Shiqiu Weng & Xiaoyun Wang

  3. Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, People’s Republic of China

    Xiaoling Ni

Authors
  1. Xiuqin Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shiqiu Weng
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiaoling Ni
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiaoyun Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xueqing Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jianbo Xiao
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Guoyin Kai
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Guoyin Kai.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Luo, X., Weng, S., Ni, X. et al. The effects of elicitation on the expression of key enzyme genes and on production of tropane alkaloids in Anisodus acutangulus plant. Biologia 67, 352–359 (2012). https://doi.org/10.2478/s11756-012-0007-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2478/s11756-012-0007-z

Key words

Search

Navigation