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Two-dimensional clinorotation influences cellular morphology, cytoskeleton and secretion of MLO-Y4 osteocyte-like cells

  • Section Cellular and Molecular Biology
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Abstract

The aim of this study was to investigate the effects of the clinostat-simulated weightlessness on biological characteristics of MLO-Y4 osteocyte-like cells. MLO-Y4 cells were incubated for 24 h, then randomly divided into 3 groups and rotated in a clinostat as a model of simulated weightlessness for 12 h, 24 h and 48 h. The morphology, cytoskeleton, and secretion of soluble molecules of MLO-Y4 cells were observed and detected. The results show that clinostat culture affects the number of dendrites/cell, cytoskeleton distribution, and secretion of nitric oxide and prostaglandin E2 in MLO-Y4 cells. These results may provide some clue to explore the cellular mechanism of bone loss caused by weightlessness.

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Abbreviations

ALP:

alkaline phosphatase

2D:

two-dimensional

CS:

calf serum

E:

experimental group

M-CSF:

macrophage colony stimulatory factor

PBS:

phosphate-buffered saline

PGE2:

prostaglandin E2

RC:

horizontal rotation control group

rpm:

revolutions per minute

SC:

stationary control group

SD:

standard deviation

TBS:

Tris buffered saline

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Authors and Affiliations

  1. Key Laboratory for Space Biosciences & Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi Xilu, Xi’an, Shaanxi Province, 710072, People’s Republic of China

    Huiyun Xu, Jiawei Wu, Yuanyuan Weng, Jian Zhang & Peng Shang

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  1. Huiyun Xu
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  2. Jiawei Wu
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  3. Yuanyuan Weng
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  4. Jian Zhang
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  5. Peng Shang
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Correspondence to Peng Shang.

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Xu, H., Wu, J., Weng, Y. et al. Two-dimensional clinorotation influences cellular morphology, cytoskeleton and secretion of MLO-Y4 osteocyte-like cells. Biologia 67, 255–262 (2012). https://doi.org/10.2478/s11756-011-0161-8

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  • DOI: https://doi.org/10.2478/s11756-011-0161-8

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