Skip to main content
SpringerLink
Log in

Impact of fusaproliferin on primary roots of maize cultivars differing in their susceptibility to Fusarium

  • Section Botany
  • Published:
Biologia Aims and scope Submit manuscript

Abstract

Effects of fusaproliferin (FUS) on membrane potential (E M), electrolyte leakage, enzymes activity and respiration of roots, were studied in two maize cultivars (Zea mays L.), differing in their susceptibility to this toxin. In short-term experiments (≤ 6 h), E M has been rapidly and significantly depolarized by FUS. The rapidity of E M depolarization in tolerant cv. Lucia was more expressive in comparison with susceptible cv. Pavla, but the extent of E M depolarization was higher in cv. Pavla. In both maize cultivars, higher depolarization of E M was registered in cells of root zone I. In long-term experiments after the first E M depolarization, which occurred during the first 6 h of FUS treatment, gradual depolarization continued up to 24 h and was represented not only by the active component (E P) but also by the passive component (E D) of E M. The decrease in E M and E D was followed by a loss of K+ ions from FUS treated roots of both cultivars. The leak of K+ ions from the root cells of both root zones as well as both maize cultivars increased with the time of FUS treatment and was significantly higher in susceptible cv. Pavla than in tolerant cv. Lucia. FUS treatment of maize roots resulted in a significant decrease of root respiration which was higher in susceptible cv. Pavla than in tolerant cv. Lucia.

The analysis of enzyme activities revealed that FUS significantly stimulated POD activity in both maize cultivars. SOD activity was significantly increased only in susceptible cv. Pavla, while APX activity was not affected by the presence of FUS. GST activity was specifically induced by FUS only in tolerant cv. Lucia.

Due to the observed correlation between the extent of depolarization and the sensitivity of the studied maize cultivars to fusaproliferin, the E M parameters should be used for rapid screening of FUS-resistant cultivars for agricultural practice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

APX:

ascorbate peroxidase

FC:

fusicoccine

FUS:

fusaproliferin

GST:

glutathione-S-transferase

PM:

plasma membrane

POD:

guajacol peroxidase

SOD:

superoxide dismutase

References

  • Atkin O.K., Edwards E.J. & Loveys B.R. 2000. Response of root respiration to changes in temperature and its relevance to global warming. New Phytol. 147: 141–154.

    Article  CAS  Google Scholar 

  • D’Alton A. & Etherton B. 1984. Effect of fusaric acid on tomato root hair membrane potentials and ATP levels. Plant Physiol. 74: 39–42.

    Article  PubMed  Google Scholar 

  • DeGara L., de Pinto M.C. & Tommasi F. 2003. The antioxidant system vis-a-vis reactive oxygen species during plantpathogen interaction. Plan Physiol. Biochem. 41: 863–870.

    Article  CAS  Google Scholar 

  • Beyer W.F. & Fridovich I. 1987. Assaying for superoxide dismutase activity: some large consequence of minor changes in conditions. Anal. Biochem. 161: 559–566.

    Article  PubMed  CAS  Google Scholar 

  • Bradford M.N. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.

    Article  PubMed  CAS  Google Scholar 

  • Chance B. & Maehly A.C. 1955. Assay of catalases and peroxidases, pp. 764–775. In: Colowick P.S. & Kaplan N.O. (eds), Methods in Enzymology, Vol. 2, Academic Press, New York.

    Chapter  Google Scholar 

  • Chen G. & Asada K. 1989. Ascorbate peroxidase in tea leaves: occurrence of two isozymes and the differences in their enzymatic and molecular properties. Plant Cell Physiol. 30: 987–998.

    CAS  Google Scholar 

  • Desjardins A.E. & Hohn T.M. 1997. Mycotoxins in plant pathogenesis. Molec. Plant-Microbe Interactions 10: 147–152.

    Article  CAS  Google Scholar 

  • Edwards R., Dixon D.P. & Walbot V. 2000. Plant glutathione-Stransferases: enzymes with multiple functions in sickness and in health. Trend Plant Sci. 5: 193–198.

    Article  CAS  Google Scholar 

  • Heiser I., Oßwald W. & Elstner E.F. 1998. The formation of reactive oxygen species by fungal and bacterial phytotoxins. Plant Physiol. Biochem. 36: 703–713.

    Article  CAS  Google Scholar 

  • Iannelli M.A., Pietrini F., Fiore L., Ppetrilli L. & Massacci A. 2002. Antioxidant response to cadmium in Phragmites australis plants. Plant Physiol. Biochem. 40: 977–982.

    Article  CAS  Google Scholar 

  • Köhler K. & Bentrup F.W. 1983. The effect of fusaric acid upon electrical membrane properties and ATP level in photoautotropic cell suspension cultures of Chenopodium rubrum L. Zeitschr. Pflanz. Physiol. 109: 355–361.

    Google Scholar 

  • Gäumann E. 1957. Fusaric acid as a wilt toxin. Phytopathology 47: 342–357.

    Google Scholar 

  • Lamprecht S.C., Marasas W.F.O., Alberts J.F., Cawood M., Gelderblom W.C.A. & Shepard G.S. 1994. Phytotoxicity of fumosins and AAL-Toxin to corn ant tomato. Phytopathology 84: 383–391.

    Article  CAS  Google Scholar 

  • Lemmens M., Joseph R., Schuhmacher R., Grausgruber H., Buerstmayr H., Ruckenbauer P., Neuhold G., Fidesser M. & Krska R. 1997. Head blight (Fusarium spp.) on wheat: investigation on the relationship between disease symptoms and mycotoxin content. Cereal Res. Comm. 25: 459–465.

    CAS  Google Scholar 

  • Logrieco A., Moretti A., Fornelli F., Fogliano V., Ritieni A., Caiaffa M., Randazzo G., Bottalico A. & Macchia L. 1996. Fusaproliferin production by Fusarium subglutinans and its toxicity to Artemia salina, SF - 9 insect cells, and IARC/LCL 171 human B-lymphocytes. Appl. Environ. Microbiol. 62: 3378–3784.

    PubMed  CAS  Google Scholar 

  • Marrè E. 1979. Fusicoccin: a tool in plant physiology. Annu. Rev. Plant Physiol. 30: 273–288.

    Article  Google Scholar 

  • Marrè M.T., Vergani P. & Albergoni F.C. 1993. Relationship between fusaric acid uptake and its binding to cell structures by leaves of Egeria densa and its toxic effects on membrane permeability and respiration. Physiol. Mol. Plant Pathol. 42: 141–157.

    Article  Google Scholar 

  • Mauch F. & Dudler R. 1993. Differential induction of distinct glutathione-S-transferases of wheat by xenobiotics and by pathogen attack. Plant Physiol. 102: 1193–1201.

    Article  PubMed  CAS  Google Scholar 

  • Miller J.D. & Young J.C. 1985. Deoxynivalenol in an experimental Fusarium graminearum infection of wheat. Can. J. Plant Path. 7: 132–134.

    Article  CAS  Google Scholar 

  • Nadubinská M., Ritieni A., Moretti A. & Šrobáro’a, A. 2003. Chlorophyll content in maize plants after treatment with fusariotoxins. Biologia 58: 115–119.

    Google Scholar 

  • Neuhold G., Fidesser M. & Krska R. 1977. Head blight (Fusarium spp.) on wheat: investigation on the relationship between disease symptoms and mycotoxin content. Cereal Res. Comm. 25: 459–465.

    Google Scholar 

  • Novacky A. 1980. Disease-related alteration in membrane function, pp. 369–380. In: Spanswick R.M., Lucas W.J. & Dainty J. (eds), Plant membrane transport. Current concept issues Elsevier/North Holland Biomedical Press.

  • Paciolla C., Dipierro N., Mulè G., Logrieco A. & Dipierro S. 2004. The mycotoxins beauvericin and T-2 induce cell deth and alteration to the ascorbate metabolism in tomato protoplasts. Physiol. Mol. Plant Pathol. 65: 49–56.

    Article  CAS  Google Scholar 

  • Pavlovkin J., Mistrík I., Zajchenko A.M. & Dugovič L. 1993. Some aspects of the phytotoxic action of trichothecene mycotoxin - roridin H on corn roots. Biologia 48: 435–439.

    CAS  Google Scholar 

  • Pavlovkin J., Mistrík I. & Prokop M. 2004. Some aspects of the phytotoxic action of fusaric acid on primary Ricinus roots. Plant Soil Environ. 50: 397–401.

    CAS  Google Scholar 

  • Pavlovkin J., Mistríková I., Luxová M. & Mistrík I. 2006. Effect of beauvericin on root transmembrane electric potential, electrolyte leakage and respiration of maize roots with dofferent susceptibility to Fusarium. Plant Soil Environ. 52: 492–498.

    CAS  Google Scholar 

  • Pavlovkin J., Novacky A. & Ullrich-Eberius C.I. 1986. Membrane potential changes during bacteria-induced hypersensitive reaction. Physiol. Mol. Plant Pathol. 28: 125–135.

    Article  CAS  Google Scholar 

  • Placinta C. M., D’Mello J. P. F. & Macdonald A. M. C. 1999. A rewiew of worldwide contamination of cereal grains and animal feed with Fusarium mycotoxins. Feed Sci. Technol. 78: 21–37.

    Article  CAS  Google Scholar 

  • Ritieni A., Fogliano V., Randazzo G., Scarallo A., Logrieco A. & Moretti A. 1995. Isolation and characterisation of fusaproliferin, a new toxic metabolite from Fusarium proliferatum. Natural toxin 3: 17–20.

    Article  CAS  Google Scholar 

  • Ritieni A., Monti S.M., Randazzo G., Logrieco A., Meretti A. & Peluso G. 1997. Teratogenic effects of fusaproliferin on chicken. J. Agric. Food Chem. 45: 3039–3043.

    Article  CAS  Google Scholar 

  • Santini A., Šrobárová, A., Pavlovkin J., Čiamporová, M. & Ritieni A. 2008. Fusaproliferin effects on the photosystem in the cells of maize seedlings leaves. Eur. J. Plant Pathol. 120: 363–371.

    Article  CAS  Google Scholar 

  • Scott-Craig J.S., Kerby K.B., Stein B.D. & Somerville S.C. 1995. Expression of an extracellular peroxidase that is induced in barley (Hordeum vulgare) by powdery mildew pathogen (Erysiphe graminis f. sp. hordei). Physiol. Mol. Plant Pathol. 47: 407–418.

    Article  CAS  Google Scholar 

  • Šliková, S., Šrobárová, A., Šudyová, V., Polišenská, I., Gregová E. & Mihálik D. 2010. Response of oat cultivars to Fusarium infection with a view to their suitability for food use. Biologia 65: 609–614.

    Article  Google Scholar 

  • Snijders C.H.A. & Perkowski J. 1990. Effect of head blight caused by Fusarium culmorum on toxin content and weight of wheat kernels. Phytopathology 80: 566–570.

    Article  CAS  Google Scholar 

  • Šrobárová, A., Nadubinská M. & Čiamporoá, M. 2004. Relative efficacy of fusariotoxins on young maize plants. Cereal Res. Comm. 2: 241–248.

    Google Scholar 

  • Šrobároá, A., Teixiera da Silva J. A., Kogan G., Ritieni A. & Santini A. 2009. Beauvericin dcreases cell viability of wheat. Chem. Biodiver. 6: 1208–1215.

    Article  Google Scholar 

  • Tamás, L., Dudíková, J., Ďurčeková, K., Huttová, J., Mistrík, I., & Zelinová V. 2008. The impact of heavy metals on the activity of some enzymes along the barley root. Env. Exp. Bot. 62: 86–91.

    Article  Google Scholar 

  • van Loon L.C. 1985. Pathogenesis-related proteins. Plant Mol. Biol. 4: 111–116.

    Article  Google Scholar 

  • Vianello M. & Macri F. 1978a. Inhibition of plant cell membrane transport phenomena induced by zearalenone. Planta 143: 51–57.

    Article  CAS  Google Scholar 

  • Vianello M. & Macri F. 1978b. Effect of zearalenone (F-2) on pea steam, maize root and rat liver mitochondria. Planta 153: 443–446.

    Article  Google Scholar 

  • Wang Y.Z. & Miller J.D. 1988. Effect of Fusarium graminearum metabolites on wheat tissue in relation to Fusarium head blight resistance. J. Phytopathol. 122: 118–125.

    Article  CAS  Google Scholar 

  • Ziegler W., Pavlovkin J., Raňansky J. & Zajchenko A.M. 1994. Ion channels induced by some mycotoxins in lipid bilayer membranes. Biologia 49: 855–862.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84523, Bratislava, Slovakia

    Ján Pavlovkin, Katarína Jašková & Ladislav Tamás

  2. Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University, Odbojárov 10, SK-83232, Bratislava, Slovakia

    Ingrid Mistríková

Authors
  1. Ján Pavlovkin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Katarína Jašková
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ingrid Mistríková
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ladislav Tamás
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ján Pavlovkin.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pavlovkin, J., Jašková, K., Mistríková, I. et al. Impact of fusaproliferin on primary roots of maize cultivars differing in their susceptibility to Fusarium . Biologia 66, 1044–1051 (2011). https://doi.org/10.2478/s11756-011-0114-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2478/s11756-011-0114-2

Key words

Search

Navigation