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Biologia

, Volume 66, Issue 4, pp 618–625 | Cite as

Induction of Gentiana cruciata hairy roots and their secondary metabolites

  • Sadiye HaytaEmail author
  • Aynur Gurel
  • Ismail Hakkı Akgun
  • Filiz Altan
  • Markus Ganzera
  • Bahattin Tanyolac
  • Erdal Bedir
Section Cellular and Molecular Biology
  • 249 Downloads

Abstract

Gentiana cruciata L. (cross gentian) is a medicinal and ornamental plant. The root extracts of this species are known to exhibit many curative properties. The natural Gentiana populations are exposed to great danger because of their uncontrolled usage. In this study, hairy roots from Gentiana cruciata L. stem and leaf explants belonging to three different clones were induced by inoculation with four different Agrobacterium rhizogenes wild strains namely A4, 15834, 8196 and R1000. Induction of the root transformation was significantly dependent on the explant type used. On the other hand, the genotype and bacterial strain had no significant effect on hairy root formation. Hairy root formation percentages of the explants varied between 5.6–33.3% in the stem explants, and between 0.0–6.7% in the leaf explants. Transformations of the hairy roots were confirmed by PCR using rolC specific primers, and revealed the absence of contaminating A. rhizogenes with virC primers. Total of twelve hairy root clones were obtained, and their secondary metabolite content was also analyzed by HPLC. Quantitative results exhibited that gentiopicroside was the most abundant compound in all root samples. Furthermore, metabolites such as loganic acid, swertiamarin, and sweroside were also identified and quantified in the samples.

Key words

Gentiana cruciata L. hairy root cultures Agrobacterium rhizogenes secoiridoid-glycosides HPLC 

Abbreviations

FGI

fresh growth index

LB

Luria-Bertani

MS

Murashige-Skoog

MSB

MS salts and B5 vitamins

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Copyright information

© © Versita Warsaw and Springer-Verlag Wien 2011

Authors and Affiliations

  • Sadiye Hayta
    • 1
    Email author
  • Aynur Gurel
    • 1
  • Ismail Hakkı Akgun
    • 1
  • Filiz Altan
    • 1
  • Markus Ganzera
    • 2
  • Bahattin Tanyolac
    • 1
  • Erdal Bedir
    • 1
  1. 1.Faculty of Engineering, Department of Bioengineering, BornovaEge UniversityIzmirTurkey
  2. 2.Institute of Pharmacy — PharmacognosyUniversity of InnsbruckInnsbruckAustria

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