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Tubulin polymerization promoting protein (TPPP) ortholog from Suberites domuncula and comparative analysis of TPPP/p25 gene family

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Abstract

Sponges are one of the oldest metazoan phyla that are, due to their highly conservative nature, often referred to as the living fossils of multicellular animals. As such, they are a very important model for evolutionary, developmental and functional studies of Metazoa. Tubulin polymerization promoting proteins (TPPPs) are defined by the presence of p25-alpha domain (Pfam05517). Their functional characteristics resemble those of microtubule-associated proteins. Presence of TPPP homologous genes has been postulated in all eukaryotes with ciliated cells and their primary function has been proposed as some basic cilia-connected function. We present here the genomic structure and the corresponding cDNA sequence of one poriferan TPPP homolog (SdTPPP) isolated from the marine sponge Suberites domuncula; and a comparative analysis of TPPP homolog sequences and genomic structures from other Eukaryotes. Our results confirm the radiation of one TPPP homolog into three distinct genes in the Vertebrate lineage, but the origin of different sequences and their phylogenetic relationships show to be influenced by alternative protein isoforms, independent gene duplications, modularity of the p25-alpha domain and possible adaptational requirements to environmental conditions.

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Abbreviations

EST:

expressed sequence tag

MAP:

microtubule associated protein

Myr:

million years

NCBI:

National Center for Biotechnology Information

TPPP:

tubulin polymerization promoting protein

Plavi:

Plasmodium vivax

Plafa:

Plasmodium falciparum

Toxgo:

Toxoplasma gondii

Tetth:

Tetrahymena thermophyla

Parte:

Paramecium tetraurelia

Leiin:

Leishmania infantum

Trycr:

Trypanosoma cruzi

Chlre:

Chlamydomonas reinhardtii

Monbr:

Monosiga brevicollis

Batde:

Batrachochytrium dendrobatidis

Subdo:

Suberites domuncula

Nemve:

Nematostella vectensis

Strpu:

Strongylocentrotus purpuratus

Ampqu:

Amphimedon queenslandica

Hydma:

Hydra magnapapillata

Cioin:

Ciona intestinalis

Trica:

Tribolium castaneum

Dromo:

Drosophila mojavensis

Drowi:

Drosophila willistoni

Drops:

Drosophila pseudoobscura

Droya:

Drosophila yakuba

Drome:

Drosophila melanogaster

Nasvi:

Nasonia vitripennis

Apime:

Apis mellifera

Anoga:

Anopheles gambiae

Aedae:

Aedes aegypti

Culqu:

Culex quinquefasciatus

Caeel:

Caenorhabditis elegans

Bruma:

Brugia malayi

Brafl:

Branchiostoma floridae

Musmu:

Mus musculus

Homsa:

Homo sapiens

Tetni:

Tetraodon nigroviridis

Osmmo:

Osmerus mordax

Xenla:

Xenopus laevis

Xentr:

Xenopus tropicalis

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Authors and Affiliations

  1. Ruđer Bošković Institute, Center for Marine Research, Giordano Paliaga 5, HR-52210, Rovinj, Croatia

    Mauro Štifanić & Renato Batel

  2. Institute for Physiological Chemistry and Pathobiochemistry, Applied Molecular Biology, Johannes Gutenberg University, Duesbergweg 6, D-55128, Mainz, Germany

    Werner E. G. Müller

Authors
  1. Mauro Štifanić
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  2. Renato Batel
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  3. Werner E. G. Müller
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Correspondence to Mauro Štifanić.

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Štifanić, M., Batel, R. & Müller, W.E.G. Tubulin polymerization promoting protein (TPPP) ortholog from Suberites domuncula and comparative analysis of TPPP/p25 gene family. Biologia 66, 111–120 (2011). https://doi.org/10.2478/s11756-010-0147-y

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