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Susceptibility of some clinical isolates of Staphylococcus aureus to fractions from the aerial parts of Leuzea carthamoides

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Abstract

The antimicrobial activity of the dichloromethane extract from aerial parts of Leuzea carthamoides DC. was tested in vitro against 19 Staphylococcus aureus strains (ATCC 25923, CNCTC Mau 43/60, clinical isolates). The extract was fractionated by column chromatography on silica gel into six fractions (petroleum ether, toluene, dichloromethane, ethyl acetate, methanol and water). The minimum inhibitory concentrations (MICs) of the fractions ranged from 64 to 1024 µg/mL. An ethyl acetate fraction (EA 1) with the widest range of activity inhibited all of the strains with MIC in the range 128–512 µg/mL. This fraction exhibited potent activity against strains which showed associated resistance to oxacillin, ciprofloxacin and erythromycin.

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Authors and Affiliations

  1. Department of Gene Bank, Division of Plant Genetics, Breeding and Quality Control, Crop Research Institute, Drnovská 507, CZ-16106, Prague 6, Czech Republic

    Dagmar Janovská

  2. Department of Crop Production, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Kamýcká 129, CZ-16521, Prague 6, Czech Republic

    Pavel Klouček

  3. Centre of Epidemiology and Microbiology, National Institute of Public Health, Šrobárova 48, CZ-10042, Prague 10, Czech Republic

    Jan Urban

  4. Laboratory of Plant Biotechnologies, Joint Laboratory of Institute of Experimental Botany Academy of Sciences of the Czech Republic and Crop Research Institute, Drnovská 507, CZ-16106, Prague 6, Czech Republic

    Tomáš Vaněk

  5. Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Kamýcká 129, CZ-16521, Prague 6, Czech Republic

    Vojtěch Rada

  6. Department of Tropical and Subtropical Crops and Agroforestry, Institute of Tropics and Subtropics, Czech University of Life Sciences, Kamýcká 129, CZ-16521, Prague 6, Czech Republic

    Ladislav Kokoška

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  1. Dagmar Janovská
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  2. Pavel Klouček
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  3. Jan Urban
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  4. Tomáš Vaněk
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  5. Vojtěch Rada
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  6. Ladislav Kokoška
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Correspondence to Ladislav Kokoška.

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Janovská, D., Klouček, P., Urban, J. et al. Susceptibility of some clinical isolates of Staphylococcus aureus to fractions from the aerial parts of Leuzea carthamoides . Biologia 63, 607–609 (2008). https://doi.org/10.2478/s11756-008-0105-0

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  • DOI: https://doi.org/10.2478/s11756-008-0105-0

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