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Tetraploids Isatis indigotica are more responsive and adaptable to stresses than the diploid progenitor based on changes in expression patterns of a cold inducible Ii CPK1

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Abstract

In plants, Ca2+-dependent protein kinases (CDPKs) are characterized as important sensors of Ca2+ flux in response to varieties of biotic and abiotic stress. A comprehensive survey of global gene expression performed by using an Arabidopsis thaliana whole genome Affymetrix gene chip revealed that CDPK tends to be significantly higher in tetraploid Isatis indigotica than in diploid ones. To investigate different CDPK expression in response to polyploidy, a full-length cDNA clone (IiCPK1) encoding CDPK was isolated from the traditional Chinese medicinal herb I. indigotica cDNA library. IiCPK1 contains some basic features of CDPKs: a catalytic kinase domain including an ATP-binding domain and four EFhand calcium-binding motifs. Real-time PCR analysis indicated the expression of IiCPK1 from two kinds of I. indigotica (tetraploid and diploid). They both were induced in response to cold stress, but tetraploids I. indigotica which has good fertility, exhibited an enhanced resistance and higher yield, and presented to be more responsive and adaptable. Our results suggest that IiCPK1 gene plays a role in adapting to the environmental stress.

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Abbreviations

ATH1:

Arabidopsis thaliana whole genome Affymetrix gene chip

CDPKs:

Ca2+ dependent protein kinases

GSP:

gene specific primer

IiCPK1:

Ca2+-dependent protein kinases of Isatis indigotica

RACE:

rapid amplification of cDNA ends

TCM:

traditional Chinese medicine

UPM:

universal primer

References

  • Altschul S.F., Madden T.L., Schäffer A.A., Zhang J., Zhang Z., Miller W. & Lipman D.J. 1997. Gapped BLAST and PSIBLAST: a new generation of protein database search programs. Nucleic Acids Res 25: 3389–3402.

    Article  PubMed  CAS  Google Scholar 

  • Benson D.A., Karsch-Mizrachi I., Lipman D.J., Ostell J. & Wheeler D.L. 2007. GenBank. Nucleic Acids Res. 35 (Database issue): D21–D25.

    Article  PubMed  CAS  Google Scholar 

  • Gasteiger E., Gattiker A., Hoogland C., Ivanyi I., Appel R.D. & Bairoch A. 2003. ExPASy: the proteomics server for indepth protein knowledge and analysis. Nucleic Acids Res 31: 3784–3788. Harmon A.C., Gribskov M. & Harper J.F. 2000. CDPKs — a kinase for every Ca2+ signal? Trends Plant Sci. 5: 154–159.

    Article  PubMed  CAS  Google Scholar 

  • Harper J.F., Bimder B.M. & Sussman M.R. 1993. Calcium and lipid regulation of an A. thaliana protein kinase expressed in Escherichia coli. Biochemistry 32: 3282–3290.

    Article  PubMed  CAS  Google Scholar 

  • Harper J.F., Sussman M.R., Schaller G.E., Putnam-Evans C., Charbonneau H. & Harman A.C. 1991. A calcium-dependent protein kinase with a regulatory domain similar to calmodulin. Science 252: 951–954.

    Article  PubMed  CAS  Google Scholar 

  • Hrabak E.M., Chan C.W., Gribskov M., Harper J.F., Choi J.H., Halford N., Kudla J., Luan S., Nimmo H.G., Sussman M.R., Thomas M., Walker-Simmons K., Zhu J.K., Harmon A.C. 2003. The Arabidopsis CDPK-SnRK superfamily of protein kinases. Plant Physiol. 132: 666–680.

    Article  PubMed  CAS  Google Scholar 

  • Jaakola L., Pirtila A.M., Halonen M. & Hohtola A. 2001. Isolation of high quality RNA from bilberry (Vaccinium myrtillus L.) fruit. Mol. Biotechnol. 19: 201–203.

    Article  PubMed  CAS  Google Scholar 

  • Kashkush K., Feldman M. & Levy A.A. 2003. Transcriptional activation of retrotransposons alters the expression of adjacent genes in wheat. Nat. Genet. 33: 102–106.

    Article  PubMed  CAS  Google Scholar 

  • Kumar S., Tamura K. & Nei M. 2004. MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief. Bioinform. 5: 150–163.

    Article  PubMed  CAS  Google Scholar 

  • Lee H.S. & Chen Z.J. 2001. Protein-coding genes are epigenetically regulated in Arabidopsis polyploids. Proc. Natl. Acad. Sci. USA 98: 6753–6758.

    Article  PubMed  CAS  Google Scholar 

  • Llop-Tous I., Dominguez-Puigjaner E. & Vendrell M. 2002. Characterization of a strawberry cDNA clone homologous to calcium-dependent protein kinases that is expressed during fruit ripening and affected by low temperature. J. Exp. Bot. 53: 2283–2285.

    Article  PubMed  CAS  Google Scholar 

  • Lu B.B., Pan X.Z., Zhang L., Huang B.B., Sun L.N., Li B., Yi B., Zheng S.Q., Yu X.J., Ding R.X. & Chen W.S. 2006. Genes responsive to autopolyploidy in Isatis indigotica using Arabidopsis thaliana affymetrix genechips. Plant Mol. Biol. Rep. 24: 197–204.

    Article  CAS  Google Scholar 

  • Qiao C.Z. & Li H. 1994. Cultivation and popularization for tetraploidy strain of Isatis indigotica. Chinese Traditional Herb 17: 3–6.

    Google Scholar 

  • Qiao C.Z., Wu M.S., Dai F.B., Cui X. & Li L. 1989. Studies on polyploid breeding of Isatis indigotica Fort. Acta Botanica Sinica 31: 678–683.

    Google Scholar 

  • Romeis T., Ludwig A., Martin R. & Jones J. 2001. Calciumdependent protein kinases play an essential role in a plant defence response. EMBO J. 20: 5556–5567.

    Article  PubMed  CAS  Google Scholar 

  • Rudd J.J. & Franklin-Tong V.E. 2001. Unravelling response-specificity in Ca2+ signaling pathways in plant cells. New Phytol. 151: 7–33.

    Article  CAS  Google Scholar 

  • Saijo Y., Hata S., Sheen J. & Izui K. 1997. cDNA cloning and prokaryotic expression of maize calcium-dependent protein kinases. Biochim. Biophys. Acta 1350: 109–114.

    PubMed  CAS  Google Scholar 

  • Schranz M.E. & Osborn T.C. 2000. Novel flowering time and variation in the resynthesized polyploid Brassica napus. J. Heredity 91: 242–246.

    Article  CAS  Google Scholar 

  • Siow Y.L., Gong Y., Au-Yeung K.K., Woo C.W. & Choy P.C. 2005. Emerging issues in traditional Chinese medicine. Can. J. Physiol. Pharmacol. 82: 321–334.

    Article  Google Scholar 

  • The Arabidopsis Initiative 2000. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408: 796–815.

    Article  Google Scholar 

  • Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F. & Higgins D.G. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24: 4876–4882.

    Article  Google Scholar 

  • Urao T., Katagiri T., Mizoguchi T., Yamaguchi-Shinozaki K., Hayashida N. & Shinozaki K. 1994. An Arabidopsis thaliana cDNA encoding Ca2+-dependent protein kinase. Plant Physiol. 105: 1461–1462.

    Article  PubMed  CAS  Google Scholar 

  • Wang Y., Qiao C.Z., Liu S. & Hang H.M. 2000. Evaluation on antiendotoxic action and antiviral action in vitro of tetraploid Isatis indigotica. Chinese Traditional Herb 25: 327–329.

    CAS  Google Scholar 

  • Wu Y.C., Hsu J.H., Liu I.M., Liou S.S., Su H.C. & Cheng J.T. 2002. Increase of insulin sensitivity in diabetic rats received Die-Huang-Wan, a herbal mixture used in Chinese traditional medicine. Acta Pharmacologica Sinica 23: 1181–1187.

    PubMed  CAS  Google Scholar 

  • Xie C., Kokubun T., Houghton P.J. & Simmonds M.S. 2004. Antibacterial activity of the Chinese traditional medicine, Zi Hua Di Ding. Phytother. Res. 18: 497–500.

    Article  PubMed  CAS  Google Scholar 

  • Yoon G.M., Cho H.S., Ha H.J., Liu J.R. & Lee H.S.P. 1999. Characterization of NtCDPK1, a calcium-dependent protein kinase gene in Nicotiana tabacum, and the activity of its encoded protein. Plant Mol. Biol. 39: 991–1001.

    Article  PubMed  CAS  Google Scholar 

  • Zhang B., Wang Z.F., Tang M.Z. & Shi Y.L. 2005. Growth inhibition and apoptosis-induced effect on human cancer cells of toosendanin, a triterpenoid derivative from Chinese traditional medicine. Invest. New Drugs 23: 547–553.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai, 200433, Peoples Republic of China

    Xinzhu Pan, Zinan Wang & Kexuan Tang

  2. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai, 200433, Peoples Republic of China

    Ying Xiao & Lei Zhang

  3. Plant Biotechnology Research Center, School of Agriculture and Biology, Fadan-SJTU-Nottingham Plant Biotechnology R&D Center, Shanghai Jiao Tong University, Shanghai, 200240, Peoples Republic of China

    Kexuan Tang

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  1. Xinzhu Pan
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  2. Ying Xiao
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  3. Zinan Wang
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  4. Lei Zhang
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  5. Kexuan Tang
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Correspondence to Lei Zhang or Kexuan Tang.

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Pan, X., Xiao, Y., Wang, Z. et al. Tetraploids Isatis indigotica are more responsive and adaptable to stresses than the diploid progenitor based on changes in expression patterns of a cold inducible Ii CPK1. Biologia 63, 535–541 (2008). https://doi.org/10.2478/s11756-008-0094-z

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