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Antifungal activity of methanol extracts from spikes of Triticum spelta and Triticum aestivum genotypes differing in their response to Fusarium culmorum inoculation

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Abstract

The total concentrations of free phenolic compounds and peroxidase were determined in spikes (collected at the flowering stage) of some spelt and common wheat cultivars differing in their response to F. culmorum infection. The antifungal activity of methanol extracts obtained from spikes was also evaluated. The tested genotypes differed significantly in their response to inoculation. The most resistant were Torka and Zebra among common wheat cultivars, and Weisser Grannenspelz among spelt cultivars. The average content of free phenolic compounds in spikes of spelt and common wheat was 1246.56 μg g−1 and 1236.58 μg g−1, respectively. The cultivars whose spikes contained the largest amounts of phenols showed the weakest response to F. culmorum infection. No significant differences were observed with regard to peroxidase content, which was 5.22 U g−1 in common wheat spikes and 5.14 U g−1 in spelt spikes. Methanol extracts from spikes of all wheat cultivars contained antifungal substances. The extracts from spelt spikes inhibited the growth of F. culmorum on PDA to a lesser degree than the extracts from common wheat spikes. This corresponds to the results of field trials, in which T. spelta generally exhibited a stronger response to F. culmorum infection than common wheat. The high correlation (r = 0.816) between mycelium growth inhibition on the medium and F. culmorum infection indicates that an evaluation of the antifungal activity of extracts from spikes may be used for the selection of breeding materials directed towards increased resistance to Fusarium head blight.

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References

  • Abdel-Aal E.-S.M., Hucl P., Sosulski F., Graf R., Gillott C. & Pietrzak L. 2001. Screening spring wheat for midge resistance in relation to ferulic acid content. J. Agr. Food Chem. 49: 3559–3566.

    Article  CAS  Google Scholar 

  • Bennett R.N. & Wallsgrove R.M. 1994. Secondary metabolites in plant defence-mechanisms. New Phytol. 127: 617–633.

    Article  CAS  Google Scholar 

  • Caruso C., Chilosi G., Leonardi L., Bertini L., Magro P., Buonocore V. & Caporale C. 2001. A basic peroxidase from wheat kernel with antifungal activity. Phytochem. 58(5): 743–750.

    Article  CAS  Google Scholar 

  • Chełkowski J. 1989. Formation of mycotoxins produced by Fusarium in heads of wheat, triticale and rye, pp. 63–84. In: Chełkowski J. (ed.), Fusarium — Mycotoxins, Taxonomy and Pathogenicity. Elsevier, Amsterdam.

    Google Scholar 

  • Chrenkova M., Gálová Z., Čerešňáková Z. & Sommer A. 2001. Nutritional and biological value of spelt wheat. Acta fytotech. et zootech. 4: 191–193.

    Google Scholar 

  • Grela E.R. 1996. Nutrient composition and content of antinutritional factors in spelt (Trtiticum spelta L.) cultivars. J. Sci. Food Agr. 71: 399–404.

    Article  CAS  Google Scholar 

  • Hoffmann W., Mudra A. & Plarre W. 1985. Lehrbuch der Züchtung landwirtschaftlicher Kulturpflanzen, Band 2, 2. Aufl. Spezieller Teil. Paul Parey, Berlin und Hamburg.

    Google Scholar 

  • Klepacka J. & Fornal Ł. 2006. Ferulic Acid and its position among the phenolic compounds of wheat. Critic. Rev. Food Sci. Nutr. 46: 639–647.

    Article  CAS  Google Scholar 

  • Kunaeva R.M. 1986. Gidroliticeskie i oksilitelnye fermenty obmena fenolnych soedinenij rostenij. Izd. Nauka, Kazach. SRR, Ałma-Ata.

    Google Scholar 

  • Marconi K., Carcea M., Graziano M. & Cubadda R. 1999. Kernel properties and pasta making quality of five European spelt wheat (Triticum spelta L.) cultivars. Cereal Chem. 76(1): 25–29

    Article  CAS  Google Scholar 

  • McKeehen J.D. & Fulcher G.R. 1998. Potential role of wheat phenolic acids during grain development in Fusarium resistance. In: 2nd International Electronic Conference on Synthetic Organic Chemistry (ECSOC-2), p. 207.

  • McKeehen J.D., Busch R.H. & Fulcher R.G. 1999. Evaluation of wheat (Triticum aestivum L.) phenolic acids during grain development and their contribution to Fusarium resistance. J. Agr. Food Chem. 47: 1476–1482.

    Article  CAS  Google Scholar 

  • Mesterhazy A. 1995. Types and components of resistance to Fusarium head blight of wheat. Plant Breed. 114: 377–386

    Article  Google Scholar 

  • Metlicki L. & Ozerereckovskaja O. 1984. Biochimija immuniteta rastenija k infekcjonnym bolezniam. Izd. Nauka.

  • Mohammadi M & Kazemi H. 2002. Changes in peroxidase and polyphenol activity in susceptible and resistant wheat heads inoculated with Fusarium graminearum and induced resistance. Plant Sci. 162: 491–498.

    Article  CAS  Google Scholar 

  • Moudry J. 1999. Productivity of spelt wheat (Triticum spelta L.) spike. Scientia Agric. Bohem. 30(1): 15–26

    Google Scholar 

  • Nelson P.E., Toussoun T.A. & Marasas W.F.O. 1983. Fusarium — An Illustrated Manual for Identification. The Pennsylvania State University.

  • Parry D.W., Jenkinson, P. & McLeod L. 1995. Fusarium ear blight (scab) in small grain cereals — a review. Plant Pathol. 44: 207–238.

    Article  Google Scholar 

  • Perkowski J., Pavlová A., Šrobárová A., Stachowiak J. & Goliński P. 2002. Group B trichothecenes biosynthesis in wheat cultivars after heads inoculation with Fusarium culmorum isolates. Biologia 57: 765–771.

    CAS  Google Scholar 

  • Rozenberg R., Ruibal-Mendieta L., Petiljean G., Cani P., Delacroix D., Delzenne M., Meurens M., Quetin-Lecler Q.J. & Habib-Jiwan J.-L. 2003. Phytosterol analysis and characterization in spelt (Triticum aestivum ssp. spelta) and wheat (T. aestivum L) lipids by LC/APCI-MS. J. Cereal Sci. 38: 189–197.

    Article  CAS  Google Scholar 

  • Rüegger A., Winzeler H. & Noseberg J. 1990. Die Ertragsbildung von Dinkel (Triticum spelta L.) und Weizen (Triticum aestivum L.) unter verschiedenen Umweltbedingungen im Freiland. J. Agron. Crop Sci. 164: 145–162.

    Article  Google Scholar 

  • Schmid J.E., Winzeler M. & Winzeler H. 1994. Analysis of disease resistance and quality characters of F1 hybrids of crosses between wheat (Triticum aestivum) and spelt (Triticum spelta). Euphytica 75: 105–110.

    Article  Google Scholar 

  • Schroeder H.W. & Christensen J.J. 1963. Factors affecting resistance of wheat to scab caused by Gibberella zeae. Phytopathol. 53: 831–838.

    Google Scholar 

  • Siranidou E., Kang Z. & Buchenauer H. 2002. Studies on symptom development, phenolic compounds and morphological defence responses in wheat cultivars differing in resistance to Fusarium Head Blight. J. Phytopathol. 150: 200–2008.

    Article  Google Scholar 

  • Snijders C.H.A. 1990. The inheritance of resistance to head blight caused by Fusarium culmorum in winter wheat. Euphytica 50: 11–18.

    Article  Google Scholar 

  • Stallknecht G.F., Gilbertson K.M. & Ranney J.E. 1996. Alternative wheat cereals as food grains: einkorn, emmer, spelt, kamut and triticale, pp. 156–170. In: Progress in new crops. ASHS Press, Alexandria, VA.

    Google Scholar 

  • Wieser H., Kieffer R., Mück U. & Reents H.J. 1998. Einfluss von Sorte und Standort auf Ertrag und Qualität von Dinkel aus ökologischem Landbau. Ber. Deut. Forsch. Lebensmitt., pp. 208–225.

  • Wiwart M., Perkowski J., Jackowiak H., Packa D., Borusiewicz A. & Buśko M. 2004. Response of some cultivars of spring spelt (Triticum spelta) to Fusarium culmorum infection. Die Bodenkultur 55(3): 103–111.

    Google Scholar 

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Authors and Affiliations

  1. Department of Plant Breeding and Seed Production, University of Warmia and Mazury, pl. Łódzki 3, PL-10-724, Olsztyn, Poland

    Elżbieta Suchowilska, Marian Wiwart & Grażyna Grabowska

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  1. Elżbieta Suchowilska
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  2. Marian Wiwart
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  3. Grażyna Grabowska
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Correspondence to Marian Wiwart.

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Suchowilska, E., Wiwart, M. & Grabowska, G. Antifungal activity of methanol extracts from spikes of Triticum spelta and Triticum aestivum genotypes differing in their response to Fusarium culmorum inoculation. Biologia 63, 471–476 (2008). https://doi.org/10.2478/s11756-008-0075-2

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