Abstract
Oxidative stress causes damage to proteins, lipids and nucleic acids, and thereby compromises cell viability. Some of the oxidative stress markers in an eukaryotic model organism, fission yeast Schizosaccharomyces pombe, were evaluated in this study. Intracellular oxidation, protein carbonyls, lipid peroxidation and reduced glutathione (GSH) levels were investigated in H2O2-treated and non-treated control cells. It was observed that increased H2O2 concentration proportionally lowered the cell number and increased the intracellular oxidation, lipid peroxidation and protein carbonyl levels in S. pombe. A dose-dependent decrease in GSH level was also detected. The fission yeast S. pombe is best known for its contribution to understanding of eukaryotic cell cycle control. S. pombe displays a different physiology from Saccharomyces cerevisiae in several ways and is thus probably more closely related to higher eukaryotes. The purpose of this study was to provide some data about the effects of hydrogen peroxide on the proteins and lipids in the fission yeast. The data obtained here is expected to constitute a basis for the further studies on redox balance and related processes in yeast and mammalian cells.
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Abbreviations
- DCFH:
-
2,7-dichlorofluorescein
- DCFH-DA:
-
2,7-dichlorofluorescein diacetate
- GSH:
-
glutathione
- MDA:
-
malondialdehyde
- ROS:
-
reactive oxygen species
- TBARS:
-
thiobarbituric acid reactive substances
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Pekmez, M., Arda, N., Hamad, İ. et al. Hydrogen peroxide-induced oxidative damages in Schizosaccharomyces pombe . Biologia 63, 151–155 (2008). https://doi.org/10.2478/s11756-008-0040-0
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DOI: https://doi.org/10.2478/s11756-008-0040-0