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Total lipid and fatty acid compositions of Lertha sheppardi (Neuroptera: Nemopteridae) during its main life stages

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Abstract

Total lipid and the fatty acid compositions of phospholipid and triacylglycerol fractions, prepared from eggs, 3rd instars of larvae, pupae, male and female adults of Lertha sheppardi, were analyzed by gas chromatography and gas chromatography-mass spectrometry. The effect of diet (adults’ nutrition) on fatty acid composition of L. sheppardi adults was also investigated. Total lipid of L. sheppardi considerably increased in adults compared with immature stages. There was a significant decrease in total lipid level in larval stage in contrast with egg stage. Qualitative analysis revealed the presence of 14 fatty acids during all stages. The major components were C16 and C18 saturated and unsaturated components which are ubiquitous to most animal species. In addition to these components, one odd-chain (C17:0) and prostaglandin precursor fatty acids were found. The fatty acid profiles of phospholipids and triacylglycerols were substantially different. In phospholipid fraction, monounsaturated fatty acids were the major proportion of fatty acids in both sex of adults and pupae, whereas polyunsaturated fatty acids were the most dominant fatty acids in eggs and 3rd instars. Results of triacylglycerol fraction revealed that fatty acid composition of eggs had higher level of C16:1, C18:0 and C18:3n-3 content than that of 3rd instars and pupae, which suggests accumulation of energetic and structural reserve materials during embryonic development. At more advanced developmental stages, mainly in adult females, the amount of C16:1 increased once again, which may be related to the need for accumulation of sufficient energy and of carbon reservoir in the developing new vitellum. Percentages of C18:1 were significantly high in adult stages compared to other stages. These findings indicate that the accumulation and consumption of fatty acids fluctuate through different development stages. Diet did not effect the fatty acid composition of L. sheppardi adults.

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Abbreviations

FA:

fatty acid

FAME:

fatty acid methyl ester

MUFA:

monounsaturated fatty acid

PL:

phospholipid

PUFA:

polyunsaturated fatty acid

SFA:

saturated fatty acid

TG:

triacylglycerol

TLC:

thin-layer chromatography

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Authors and Affiliations

  1. Department of Biology, Education Faculty, Dicle University, 21280, Diyarbakir, Turkey

    Ozlem Cakmak

  2. Department of Biology, Science and Art Faculty, Dicle University, 21280, Diyarbakir, Turkey

    Mehmet Bashan & Ali Satar

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  1. Ozlem Cakmak
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  2. Mehmet Bashan
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  3. Ali Satar
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Correspondence to Ozlem Cakmak.

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Cakmak, O., Bashan, M. & Satar, A. Total lipid and fatty acid compositions of Lertha sheppardi (Neuroptera: Nemopteridae) during its main life stages. Biologia 62, 774–780 (2007). https://doi.org/10.2478/s11756-007-0147-8

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