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Effects of Enterococcus faecium and Bacillus cereus var. toyoi on the morphology of the intestinal mucous membrane in piglets

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Abstract

Eighty piglets aged 14, 28, 35 and 56 days — weaned at day 28 — were subjected to this investigation. Each age-group consisted of five animals which were fed an Enterococcus faecium NCIMB 10415 (Cylactin®) and Bacillus cereus var. toyoi (Toyocerin®) based diet. Five animals served as controls. Tissue samples were collected immediately after sacrifice at 8.30 h a.m. from duodenum, jejunum, ileum, cecum and colon to examine intestinal morphology and histochemistry. The results showed that with respect to villus height and crypt depth supplementation of probiotics in piglets feed seemed to influence the morphology and enlargement factor not at all or only to a certain extent. With respect to the number of goblet cells, the difference between probiotic fed animals and control animals was generally extremely low. The shape of the villi of the small intestinal segments greatly varied in all age groups of control and probiotic fed animals. However, this morphological variety does not depend on the mode of feeding.

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References

  • Baum, B., Liebler-Tenorio, E.M., Enss, M.L., Pohlenz, J.F. & Breves, G. 2002. Saccharomyces boulardii and Bacillus cereus var. toyoi influence the morphology and the mucins of the intestine of pigs. Z. Gastroenterol. 40: 277–284.

    Article  PubMed  CAS  Google Scholar 

  • Bengmark, S. 2000. Colonic food: pre-and probiotics. Am. J. Gastroenterol. 95(Suppl): 5–7.

    Article  Google Scholar 

  • Bocourt, R., Savon, L., Diaz, J., Brizuela, M.A, Serrano, P., Prats, A. & Elias, A. 2004. Effect of the probiotic activity of Lactobacillus rhamnosus on productive and health indicators of piglets. Cuban J. Agric. Sci. 38: 75–79.

    Google Scholar 

  • Cera, K.R., Mahan, D.C., Cross, R.F., Reinhart, G.A. & Whitemoyer, R.E. 1988. Effect of age, weaning and post-weaning diet on small intestinal growth and jejunal morphology in young swine. J. Anim. Sci. 66: 574–584.

    PubMed  CAS  Google Scholar 

  • Di Giancamillo, A., Bontempo, V., Savoini, G., Paratte, R., Cheveau, E., Dell’orto, V. & Domeneghini C. 2003. In: 9th International Symposium on Digestive Physiology in Pigs, Banff, AB, Canada. 2: 262–264.

    Google Scholar 

  • Fernandez-Estivariz, C., Gu, L.H., Gu, L., Jonas, C.R., Wallace, T.M., Pascal, R.R., Devaney, K.L., Farrell, C.L., Jones, D.P., Podolsky, D.K. & Ziegler T.R. 2003. Trefoil peptide expression and goblet cell number in rat intestine: effects of kgf and fasting-refeeding. Am. J. Physiol. Regulator. Integr. Comp. Physiol. 284: R564–R573.

    CAS  Google Scholar 

  • Fuller, R. 1989. A review: probiotics in man and in animals. J. Appl. Bacteriol. 66: 365–378.

    PubMed  CAS  Google Scholar 

  • Gorbach, S. 2000. Probiotics and gastrointestinal health. Am. J. Gastroenterol. 95: 2–4.

    Article  Google Scholar 

  • Ghadben, G.S. 2002. Probiotics in broiler production — a review. Arch. Geflugelkunde 66: 49–58.

    Google Scholar 

  • Gibson, G.R. 2004. From probiotics to prebiotics and a healthy digestive system. J. Food Sci. 69: M141–M143.

    Article  CAS  Google Scholar 

  • Görke, B. 2000. Untersuchungen der Schleimhautmorphologie im Dünn-und Dickdarm nach oraler Applikation von Saccaromyces boulardii und Bacillus cereus var. toyoi beim Schwein. Vetmed dissertation Hannover, Germany.

    Google Scholar 

  • Gu, X. & Li, D. 2004. Effect of dietary crude protein level on villous morphology, immune status and histochemistry parameters of digestive tract in weaning piglets. Anim. Feed Sci. Technol. 114: 113–126.

    Article  CAS  Google Scholar 

  • Herich. R., Bomba, A., Nemcova, R. & Gancarcikova, S. 1999. The influence of short-term and continuous administration of Lactobacillus casei on basic haematological and immunological parameters in gnotobiotic piglets. Food Agric. Immunol. 11: 287–295.

    Article  Google Scholar 

  • Huang, C.H., Qiao, S.Y., Li, D.F., Piao, X.S. & Ren, J.P. 2004. Effects of lactobacilli on the performance, diarrhea incidence, VFA concentration and gastrointestinal microbial flora of weaning pigs. Asian-Austr. J. Anim. Sci. 17: 401–409.

    Google Scholar 

  • Jadamus, A., Vahjen, W., Schäfer, K. & Simon, O. 2002. Influence of the probiotic strain Bacillus cereus var. toyoi on the development of enterobacterial growth and on selected parameters of bacterial metabolism in digesta samples of piglets. J. Anim. Physiol. Anim. Nutr. 86: 42–54.

    Article  CAS  Google Scholar 

  • Jahn, H., Ullrich, R., Schneider, T., Liehr, R., Schieferdecker, L., Holst, H. & Zeitz, M. 1996. Immunological and trophical effects of Saccharomyces boulardii on the small intestine in healthy human volunteers. Digestion 57: 95–104.

    PubMed  CAS  Google Scholar 

  • Kleessen, B., Hartmann, L. & Blaut, M. 2003. Fructans in the diet cause alterations of intestinal mucosal architecture, released mucins and mucosa-associated bifidobacteria in gnotobiotic rats. Br. J. Nutr. 89: 597–606.

    Article  PubMed  CAS  Google Scholar 

  • Klein, U. & Schmidts, H.L. 1997. Zum Einfluss des Bioregulators Paciflor auf die Morphologie der Dünndarmmukosa beim Schwein. Proc. Soc. Nutr. Physiol. 6: 41.

    Google Scholar 

  • Lalles, J.P., Montagne, L., Plut, C. & Toullec, R. 2001. Influence of dietary protein concentration on the morphology and enzyme activities of the small intestine of the preruminant calf. Anim. Sci. 73: 571–578.

    Google Scholar 

  • Langhout, D.J., Schutte, J.B., Vanleeuwen, P., Wiebenga, J. & Tamminga, S. 1999. Effect of dietary high-and low-methylated citrus pectin on the activity of the ileal microflora and morphology of the small intestinal wall of broiler chicks. Br. Poultry Sci. 40: 340–347.

    Article  CAS  Google Scholar 

  • Marteau, P., Lepage, P., Mangin, I., Suau, A., Dore, J. Pochart, P. & Seksik, P. 2004. Gut flora and inflammatory bowel disease. Aliment. Pharmacol. Ther. 20: 18–23.

    Article  PubMed  Google Scholar 

  • Marteau, P. & Seksik, P. 2001. Probiotics and prebiotics in inflammatory bowel disease. Gastroenterologie Clinique Et Biologique 25: C94–C97.

    PubMed  CAS  Google Scholar 

  • Mekbungwan, A., Yamauchi K. & Thongwittaya, N. 2003. Histological alterations of intestinal villi in growing pigs fed soybean and pigeon pea seed meals. Can. J. Anim. Sci. 83: 755–760.

    Google Scholar 

  • Melin, L. & Wallgren, P. 2002. Aspects on feed related prophylactic measures aiming to prevent post weaning diarrhoea in pigs. Acta Vet. Scand. 43: 231–245.

    Article  PubMed  CAS  Google Scholar 

  • Miller, B.G., James, P.S., Smith, M.W. & Bourne, F.J. 1986. Effect of weaning on the capacity of pig intestinal villi to digest and adsorb nutrients. J. Agric. Sci. 107: 579–589.

    Article  Google Scholar 

  • Mitjans, M. & Ferrer, R. 2004. Morphometric study of the guinea pig small intestine during development. Microscopy Res. Techn. 63: 206–214.

    Article  Google Scholar 

  • O’sullivan, G., Kelly, C., O’Halloran, P., Collins, S., Collins, J. K., Dunne, C. & Shanahan, F. 2005. Probiotics: an emerging therapy. Curr. Pharm. Des. 11: 3–10.

    Article  PubMed  CAS  Google Scholar 

  • Piel, C., Montagne, L., Seve, B. & Lalles, J.P. 2005. Increasing digesta viscosity using carboymethylcellulose in weaned piglets stimulates ileal goblet cell numbers and maturation. J. Nutr. 135: 86–91.

    PubMed  CAS  Google Scholar 

  • Pluske, J.R., Williams, I.H. & Aherne, F.X. 1996. Villous height and crypt depth in piglets in response to increases in intake of cows’ milk after weaning. Anim. Sci. 62: 145–158.

    Article  Google Scholar 

  • Reuter, G. 2001. Probiotics — possibilities and limitations of their application in food, animal feed, and in pharmaceutical preparations for man and animals. Berl. Münch. Tierärzt. Wochenschr. 114: 410–419.

    CAS  Google Scholar 

  • Romeis, B. 1989. Mikroskopische Technik, 17 Verbesserte Auflage, Urban und Schwarzenberg, München, 697 pp.

    Google Scholar 

  • Scharek, L., Guth, J., Reiter, K., Weyrauch, K.D., Taras, D., Schwerk, P., Schierack, P., Schmidt, M.F.G., Wieler, L.H. & Tedin, K. 2005. Influence of a probiotic Enterococcus faecium strain on development of the immune system of sows and piglets. Vet. Immunol. Immunopathol. 105: 151–161.

    Article  PubMed  CAS  Google Scholar 

  • Tuch, K. & Amtsberg, G. 1973. Zottenstrukturen im Dünndarm des Schweines. Stereolupenmikroskopische Befunde. Zentralblatt für Veterinärmedizin 20 A(Heft 6): 503–518.

    Google Scholar 

  • Van Leeuwen, P., Mouwen, J.M., Van Der Klis, J.D. & Verstegen, M.W.A. 2004. Morphology of the small intestinal mucosal surface of broilers in relation to age, diet formulation, small intestinal microflora and performance. Br. Poultry Sci. 45: 41–48.

    Article  Google Scholar 

  • Vente-Spreeuwenberg, M.A.M., Verdonk, J.M., Bakker, G.C.M., Beynen, A.C. & M. Verstegen, W.A. 2004. Effect of dietary protein source on feed intake and small intestinal morphology in newly weaned piglets. Livestock Prod. Sci. 86: 169–177.

    Article  Google Scholar 

  • Vahjen, W., Jadamus, A. & Simon, O. 2002. Influence of a probiotic Enterococcus faecium strain on selected bacterial groups in the small intestine of growing turkey poults. Arch. Anim. Nutr. 6: 419–129.

    Article  Google Scholar 

  • Verma, D., Malik, M.R, Shrivastava, A.M. & Parmar, M. 1999. Histogenesis of intestinal villi in fowl (Gallus domesticus). Ind. J. Anim. Sci. 69: 902–904.

    Google Scholar 

  • Wang, L., Li, J., Li, Q., Zhang, J. & Duan, X.L. 2003. Morphological changes of cell proliferation and apoptosis in rat jejunal mucosa at different ages. World J. Gastroenterol. 9: 2060–2064.

    PubMed  Google Scholar 

  • Wiese, F., Simon, O. & Weyrauch, K.D. 2003. Morphology of the small intestine of weaned piglets and a novel method for morphometric evaluation. Anat. Histol. Embryol. 32: 102–109.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Veterinary Anatomy, Freie Universität Berlin, Koserstrasse 20, D-14195, Berlin, Germany

    Katja Reiter, Susanne Eggebrecht, Barbara Drewes, Michael Riess & Karl Dietrich Weyrauch

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  1. Katja Reiter
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  2. Susanne Eggebrecht
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  3. Barbara Drewes
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  4. Michael Riess
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  5. Karl Dietrich Weyrauch
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Correspondence to Karl Dietrich Weyrauch.

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Reiter, K., Eggebrecht, S., Drewes, B. et al. Effects of Enterococcus faecium and Bacillus cereus var. toyoi on the morphology of the intestinal mucous membrane in piglets. Biologia 61, 803–809 (2006). https://doi.org/10.2478/s11756-006-0161-2

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  • DOI: https://doi.org/10.2478/s11756-006-0161-2

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