Abstract
Apple stem pitting virus (ASPV) is a causal agent of stem pitting associated disease in pomes fruit trees. The present report focuses on a cytopathological effect of ASPV infection in a herbaceous host Nicotiana occidentalis ‘37B’. A leaf dip preparation shows predominantly basic virus particles and aggregated particles 800 nm and 3200 nm long respectively. The main cytopathological effect observed in ASPV infected N. occidentalis includes fibrous aggregates of virus particles (massive/or few), formation of membranous vesicles and proliferation of the endoplasmic reticulum.
Similar content being viewed by others
References
Adams, M.J., Antoniw, J.F., Bar-Joseph, M., Brunt, A. A., Candresse, T., Foster, G.D., Martelli, G.P., Milne, R.G. & Fauquet, C.M. 2004. The new plant virus family Flexiviridae and assessment of molecular criteria for species demarcation. Arch. Virol. 149: 1045–11060.
Francki, R.I.B., Milne, R.G. & Hatta, T. 1985. Atlas of plant viruses Vol. II, pp. 219–232. CRC Press, Boca Raton, Florida.
Jelkmann, W. 1994. Nucleotide sequences of apple stem pitting virus and of the coat protein gene of a similar virus from pear associated with vein yellows disease and their relationship with potex-and carlaviruses. J. Gen. Virol. 75: 1535–1542.
Giunchedi, L. & Poggi-Pollini, C. 1992. Cytopathological, negative staining and serological electron microscopy of clostero-like virus associated with pear vein yellows disease. J. Phytopathol. 134: 329–335.
Koganezawa, H. & Yanase, H. 1990. A new type of elongated virus isolated from apple trees containing the stem pitting agent. Plant Dis. 74: 610–614.
Kundu, J.K. & Yoshikawa, N. 2006. Apple stem pitting virus. In: Rao, G.P., Myrta, A. & Ling, K-S. (eds), Molecular characterization of plant viruses. Studium Press, Houstan, Taxas, USA (in press).
Kundu, J.K. 2003a. The occurrence of Apple stem pitting virus and Apple stem grooving virus within field-grown apple cultivars evaluated by RT-PCR. Plant Prot. Sci. 39(3): 88–92.
Kundu, J.K. 2003b. A rapid and effective RNA release procedure for virus detection in woody plants by reverse transcription-polymerase chain reaction. Acta Virol. 47: 147–151.
Lemoine, J. 1979. Manifestation de symptoms de stony pit sur les fruits d’especes differentes a partir de certaines sources de vein yellows du poirier. Ann. Phytopath. 11: 519–523.
Leone, G., Lindner, J.L., Jongedijk, G., van der Meer, F.A. & Schoen, C.D. 1998. Symptoms on apple and pear indicators after back-transmission from Nicotiana occidentalis confirm the identity of Apple stem pitting virus with Pear vein yellows virus. Acta Horticult. 472: 61–65.
Lesemann, D.E. 1998. Cytopatology, pp. 179–235. In: Milne, R.G. (ed.), The plant viruses, Vol. 4: The filamentous plant viruses. Plenum Press, New York.
Lesemann, D.E. 1991. Specific cytological alterations in virus infected cells, P. 147–159. In: Mendgen, K. & Lesemann, D.E. (eds), Electron microscopy of plant pathogens. Springer-Verlag, Berlin, Heidelberg, New York.
Martelli, G.P. & Jelkmann, W. 1998. Foveavirus, a new plant genus. Arch. Virol. 143: 1245–1249.
Martelli, P.G. & Russo, M. 1984. Use of thin sectioning for visualization and identification of plant viruses. Meth. Virol. VIII: 143–224.
Nemeth, M. 1986. Virus, Mycoplasma and Rickettsia diseases of fruit trees. Martinus Nijhoff Publishers, Dordecht, Boston, Lancaster, pp. 841.
Polák, J. & Jokeš, M. 1986. Localization of the Beet mild yellowing virus in Sinapis alba L. Biol. Plant. 28: 227–229.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kundu, J.K., Ducháčová, M. & Jokeš, M. Cytopathology of Apple stem pitting virus in Nicotiana occidentalis L.. Biologia 61, 469–471 (2006). https://doi.org/10.2478/s11756-006-0078-9
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.2478/s11756-006-0078-9