Abstract
The main biologically active constituents of Hypericum species are flavonoids (quercetin, isoquercitrin, hyperoside, rutin), biflavonoids and naphthodianthrones (hypericin, pseudohypericin). Lipoxygenase is the key enzyme in the biosynthesis of leukotriens, which have been postulated to play an important role in the pathophysiology of several inflammatory and allergic diseases. This work deals with the investigation of potential antilipoxygenase activity of different compounds and extracts isolated from Hypericum perforatum L. The highest inhibitory effect was exhibited by flavonoid derivative hyperoside (IC50 5.768 × 10−6 M). Acetone and ethanolic extracts caused also an inhibition of lipoxygenase. On the basis of inhibitory effect of compounds tested we assume that the most of them may be involved in the antiinflammatory principles of Hypericum perforatum L.
References
Barnes, J., Anderson, L.A. & Phillipson, J.D. 2001. St. John wort (Hypericum perforatum L.): a review of its chemistry, pharmacology and clinical properties. J. Pharm. Pharmacol. 53: 583–600.
Bezáková, L., Obložinský, M., Sýkorová, M., Paulíková, I. & Košťálová, D. 2005. Antilipoxygenázová aktivita a obsah stopových prvkov Aloe vera vo vzťahu k terapeutickému účinku. [Antilipoxygenase activity and the trace elements content of Aloe vera in relation to the therapeutical effect]. Čes. Slov. Farm. 54: 43–46.
Bezáková, L., Pšenák, M. & Kartnig, T. 1999. Effect of dianthrones and their precursors from Hypericum perforatum L. on lipoxygenase activity. Pharmazie 54: 711–712.
Cakir, A., Mavi, A., Yildirim, A., Duru, M.E., Harmandar, M. & Kazaz, C. 2003. Isolation and characterization of antioxidant phenolic compounds from aerial parts of Hypericum hyssopifolium L. by activity-guided fractionation. J. Ethnopharmacol. 87: 73–83.
Kartnig, T., Gobel, I. & Heydel, B. 1996. Production of hypericin, pseudohypericin and flavonoids in cell cultures of various Hypericum species and their chemotypes. Planta Med. 6: 51–53.
Kemal, C., Louis-Flamberg, P., Krupinsky-Olsen, R. & Shorter, A.L. 1987. Reductive inactivation of soybean lipoxygenase 1 by catechols: a possible mechanism for regulation of lipoxygenase activity. Biochemistry 26: 7064–7072.
Kulkarni, A.P., Cai, Y. & Richards, I.S. 1992. Rat pulmonary lipoxygenase: dioxygenase activity and role in xenobiotic metabolism. Int. J. Biochem. 24: 255–261.
Lin, J.K., Tsai, S.H. & Lin-Shiau, S.Y. 2001. Antiinflammatory and antitumor effects of flavonoids and flavanoids. Drugs Future 26: 145–157.
Roginsky, V.A., Barsukova, T.K. & Remorova, A.A. 1996. Moderate antioxidative efficiencies of flavonoids during peroxidation of methyl linoleate in homogeneous and micellar solutions. JAOCS 73: 777–786.
Sroka, Z. 2005. Antioxidative and antiradical properties of plant phenolics. Z. Naturforsch [C] 60: 833–843.
Young, R.N. 1999. Inhibition of 5-lipoxygenase: a therapeutic potential yet to be fully realized? Eur. J. Med. Chem. 34: 671–685.
Zhang, H. 1998. Selection of theoretical parameter characterizing scavengering activity of antioxidants in free radicals. JAOCS 75: 1705–1709.
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Obložinský, M., Bezáková, L., Holková, I. et al. Antilipoxygenase activity of compounds from Hypericum perforatum . Biologia 61, 331–332 (2006). https://doi.org/10.2478/s11756-006-0060-6
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DOI: https://doi.org/10.2478/s11756-006-0060-6