Abstract
The aim of the research was to study how the surface coating with quaternary ammonium silanes influences the attachment of industrial yeast cells. Three brewery and distillery strains belonging to Saccharomyces cerevisiae, and one strain of Debaryomyces occidentalis with high amylolytic activity were used in this study. Native chamotte carriers were modified using two organo-silanes with different functional groups containing ethanolamine ([3-N(N,N,N-dimethyl(2-hydroxyethyl)ammonio)propyl]trimethoxysilane) and octan-1-amine (octylamine) ([3-N(N,N,N-dimethyl-octylammonio)propyl]trimethoxysilane). The yeast cell surface charge was evaluated using an alcian blue retention assay. To determine the adhesion efficiency, light microscopy and methylene blue staining of cells were used. The viability of immobilized cells was confirmed by [2-chloro-4-(2,3 dihydro-3-methyl(benzo-1,3-thiozol-2-yl)-methylidene)-1-phenylquinolinium iodide] (FUN®1) staining. Modification of chamotte carriers increased the biomass load significantly; however, organo-silane with octylamine showed strong anti-yeast properties. This paper describes the use of inexpensive porous chamotte covered with active organo-silanes with quaternary ammonium groups as a way to improve yeast cell adhesion efficiency.
Similar content being viewed by others
References
Arakaki, A., Nakazawa, H., Nemoto, M., Mori, T., & Matsunaga, T. (2008). Formation of magnetite by bacteria and its application. Journal of the Royal Society Interface, 5, 977–999. DOI: 10.1098/rsif.2008.0170.
Arkles, B., Steinmetz, J. R., Zazyczny, J., & Metha, P. (1992). Factors contributing to the stability of alkoxysilanes in aqueous solution. In K. L. Mittal (Ed.), Silanes and other coupling agents (pp. 91–104). Utrecht, The Netherlands: VSP.
Awad, A. M., Ghazy, E. A., Abo El-Enin, S. A., & Mahmoud, M. G. (2012). Electropolishing of AISI-304 stainless steel for protection against SRB biofilm. Surface and Coatings Technology, 206, 3165–3172. DOI: 10.1016/j.surfcoat.2011.11.046.
Bakalova, S., Mincheva, V., Doycheva, A., Groudeva, V., & Dimkov, R. (2008). Microbial toxicity of ethanolamines. Biotechnology & Biotechnological Equipment, 22, 716–720.
Bearinger, J. P., Stone, G., Hiddessen, A. L., Dugan, L. C., Wu, L. G., Hailey, P., Conway, J. W., Kuenzler, T., Feller, L., Cerritelli, S., & Hubbell, J. A. (2009). Phototocatalytic lithography of poly(propylene sulfide) block copolymers: Toward high-throughput nanolithography for biomolecular arraying applications. Langmuir, 25, 1238–1244. DOI: 10.1021/la802727s.
Berlowska, J., Kregiel, D., & Ambroziak, W. (2013). Enhancing adhesion of yeast brewery strains to chamotte carriers through aminosilane surface modification. World Journal of Microbiology and Biotechnology, 29, 1307–1316. DOI: 10.1007/s11274-013-1294-4.
Bučko, M., Mislovičová, D., Nahálka, J., Vikartovská, A., Šefčovičová, J., Katrlík, J., Tkáč, J., Gemeiner, P., Lacík, I., Štefuca, V., Polakovič, M., Rosenberg, M., Rebroš, M., Šmogrovičová, D., & Švitel, J. (2012). Immobilization in biotechnology and biorecognition: from macro-to nanoscale systems. Chemical Papers, 66, 983–998. DOI: 10.2478/s11696-012-0226-3.
Carré, A., Birch, W., & Lacarrière, V. (2007). Glass substrates modified with organosilanes for DNA immobilization. In K. L. Mittal (Ed.), Silanes and other coupling agents (pp. 1–14). Utrecht, The Netherlands: VSP.
Chevalier, S., Cuestas-Ayllon, C., Grazu, V., Luna, M., Feracci, H., & de la Fuente, J. M. (2010). Creating biomimetic surfaces through covalent and oriented binding of proteins. Langmuir, 26, 14707–14715. DOI: 10.1021/la103086b.
Fortuniak, W., Mizerska, U., Chojnowski, J., Basinska, T., Slomkowski, S., Chehimi, M. M., Konopacka, A., Turecka, K., & Werel, W. (2011). Polysiloxanes with quaternary ammonium salt biocidal functions and their behavior when incorporated into a silicone elastomer network. Journal of Inorganic and Organometallic Polymers and Materials, 21, 576–589. DOI: 10.1007/s10904-011-9485-7.
Garsin, D. A. (2010). Ethanolamine utilization in bacterial pathogens: roles and regulation. Nature Reviews Microbiology, 8, 290–295. DOI: 10.1038/nrmicro2334.
Gong, S. Q., Niu, L. N., Kemp, L. K., Yiu, C. K. Y., Ryou, H. J., Qi, Y. P., Blizzard, J. D., Nikonov, S., Brackett, M. G., Messer, R. L. W., Wu, C. D., Mao, J., Brister, L. B., Rueggeberg, F. A., Arola, D. D., Pashley, D. H., & Tay, F. R. (2012). Quaternary ammonium silane-functionalized, methacrylate resin composition with antimicrobial activities and self-repair potential. Acta Biomaterialia, 8, 3270–3282. DOI: 10.1016/j.actbio.2012.05.031.
Jørgensen, K., Morant, A. V., Morant, M., Jensen, N. B., Olsen, C. E., Kannangara, R., Motawia, M. S., Møller, B. L., & Bak, S. (2011). Biosynthesis of the cyanogenic glucosides linamarin and lotaustralin in cassava: Isolation, biochemical characterization, and expression pattern of CYP71E7, the oxime-metabolizing cytochrome P450 enzyme. Plant Physiology, 155, 282–292. DOI: 10.1104/pp.110.164053.
Khramov, A. N., Balbyshev, V. N., Voevodin, N. N., & Donley, M. S. (2003). Nanostructured sol-gel derived conversion coatings based on epoxy- and amino-silanes. Progress in Organic Coatings, 47, 207–213. DOI: 10.1016/s0300-9440(03)00140-1.
Kregiel, D., Berlowska, J., & Ambroziak, W. (2012a). Adhesion of yeast cells to different porous supports, stability of cellcarrier systems and formation of volatile by-products. World Journal of Microbiology and Biotechnology, 28, 3399–3408. DOI: 10.1007/s11274-012-1151-x.
Kregiel, D., Berlowska, J., & Szubzda, B. (2012b). Novel permittivity test for determination of yeast surface charge and flocculation abilities. Journal of Industrial Microbiology and Biotechnology, 39, 1881–1886. DOI: 10.1007/s10295-012-1193-y.
Kregiel, D., Berlowska, J., Mizerska, U., Fortuniak, W., Chojnowski, J., & Ambroziak, W. (2013). Chemical modification of polyvinyl chloride and silicone elastomer in inhibiting adhesion of Aeromonas hydrophila. World Journal of Microbiology and Biotechnology, 29, 1197–1206. DOI: 10.1007/s11274-013-1282-8.
Metwalli, E., Haines, D., Becker, O., Conzone, S., & Pantano, C. G. (2006). Surface characterizations of mono-, di-, and tri-aminosilane treated glass substrates. Journal of Colloid and Interface Science, 298, 825–831. DOI: 10.1016/j.jcis.2006.03.045.
Oosterhof, J. J. H., Buijssen, K. J. D. A., Busscher, H. J., van der Laan, B. F. A. M., & van der Mei, H. C. (2006). Effects of quaternary ammonium silane coatings on mixed fungal and bacterial biofilms on tracheoesophageal shunt prostheses. Applied and Environmental Microbiology, 72, 3673–3677. DOI: 10.1128/aem.72.5.3673-3677.2006.
Palermo, E. F., Lee, D. K., Ramamoorthy, A., & Kuroda, K. (2011). Role of cationic group structure in membrane binding and disruption by amphiphilic copolymers. Journal of Physical Chemistry B, 115, 366–375. DOI: 10.1021/jp1083357.
Pina-Vaz, C., Costa-de-Oliveira, S., Rodrigues, A. G., & Espinel-Ingroff, A. (2005). Comparison of two probes for testing susceptibilities of pathogenic yeasts to voriconazole, itraconazole, and caspofungin by flow cytometry. Journal of Clinical Microbiology, 43, 4674–4679. DOI: 10.1128/jcm.43.9.4674-4679.2005.
Plueddemann, E. P. (1991). Silane coupling agents. New York, NY, USA: Plenum Press.
Powell, C. D., Quain, D. E., & Smart, K. A. (2003). The impact of brewing yeast cell age on fermentation performance, atten uation and flocculation. FEMS Yeast Research, 3, 149–157. DOI: 10.1016/s1567-1356(03)00002-3.
Rapoport, A., Borovikova, D., Kokina, A., Patmalnieks, A., Polyak, N., Pavlovska, I., Mezinskis, G., & Dekhtyar, Y. (2011). Immobilisation of yeast cells on the surface of hydroxyapatite ceramics. Process Biochemistry, 46, 665–670. DOI: 10.1016/j.procbio.2010.11.009.
Saal, K., Tätte, T., Tulp, I., Kink, I., Kurg, A., Mäeorg, U., Rinken, A., & Löhmus, A. (2006). Sol-gel films for DNA microarray applications. Materials Letters, 60, 1833–1838 DOI: 10.1016/j.matlet.2005.12.035.
Safarik, I., & Safarikova, M. (2009). Magnetic nano- and microparticles in biotechnology. Chemical Papers, 63, 497–505. DOI: 10.2478/s11696-009-0054-2.
Schmidt, E. J., Boswell, J. S., Walsh, J. P., Schellenberg, M. M., Winter, T. W., Li, C. H., Allman, G. W., & Savage, P. B. (2001). Activities of cholic acid-derived antimicrobial agents against multidrug-resistant bacteria. Journal of Antimicrobial Chemotherapy, 47, 671–674. DOI: 10.1093/jac/47.5.671.
Seo, J. H., Shin, D. S., Mukundan, P., & Revzin, A. (2012). Attachment of hydrogel microstructures and proteins to glass via thiol-terminated silanes. Colloids and Surfaces B: Biointerfaces, 98, 1–6. DOI: 10.1016/j.colsurfb.2012.03.025.
Srikumar, S., & Fuchs, T. M. (2011). Ethanolamine utilization contributes to proliferation of Salmonella enterica serovar Typhimurium in food and in nematodes. Applied and Environmental Microbiology, 77, 281–290. DOI: 10.1128/aem.01403-10.
Storey, M. K., Wu, W. I., & Voelker, D. R. (2001). A genetic screen for ethanolamine auxotrophs in Saccharomyces cerevisiae identifies a novel mutation in Mcd4p, a protein implicated in glycosylphosphatidylinositol anchor synthesis. Biochimica et Biophysica Acta (BBA) — Molecular and Cell Biology of Lipids, 1532, 234–247. DOI: 10.1016/s1388-1981(01)00129-9.
Tang, H. Y., Cao, T., Wang, A. F., Liang, X. M., Salley, S. O., McAllister, J. P., & Ng, K. Y. S. (2007). Effect of surface modification of silicone on Staphylococcus epidermidis adhesion and colonization. Journal of Biomedical Materials Research, 80A, 885–894. DOI: 10.1002/jbm.a.30952.
Van Zandycke, S. M., Simal, O., & Smart, K. A. (2008). Vitality assessment using the fluorescent stain FUN1. In K. Smart (Ed.), Brewing yeast fermentation performance. Oxford, UK: Blackwell Science. DOI: 10.1002/9780470696040.ch15.
Verstrepen, K. J., & Klis, F. M. (2006). Flocculation, adhesion and biofilm formation in yeast. Molecular Microbiology, 60, 5–15. DOI: 10.1111/j.1365-2958.2006.05072.x.
Vu, D. L., Sys, M., & Červenka, L. (2011). The effect of various potentials on the attachment of Saccharomyces cerevisiae and Staphylococcus epidermidis to carbon paste electrodes. International Journal of Electrochemical Science, 6, 5265–5274.
White, J. S., & Walker, G. M. (2011). Influence of cell surface characteristics on adhesion of Saccharomyces cerevisiae to the biomaterial hydroxylapatite. Antonie Van Leeuwenhoek, 99, 201–209. DOI: 10.1007/s10482-010-9477-6.
Yamaguchi, M., Ikeda, K., Suzuki, M., Kiyohara, A., Kudoh, S. N., Shimizu, K., Taira, T., Ito, D., Uchida, T., & Gohara, K. (2011). Cell patterning using a template of microstructured organosilane layer fabricated by vacuum ultraviolet light lithography. Langmuir, 27, 12521–12532. DOI: 10.1021/la202904g.
Zeraik, A. E., & Nitschke, M. (2012). Influence of growth media and temperature on bacterial adhesion to polystyrene surfaces. Brazilian Archives of Biology and Technology, 55, 569–576. DOI: 10.1590/s1516-89132012000400012.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kregiel, D., Berlowska, J. Effect of quaternary ammonium silane coating on adhesive immobilization of industrial yeasts. Chem. Pap. 68, 308–315 (2014). https://doi.org/10.2478/s11696-013-0462-1
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.2478/s11696-013-0462-1