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Study of deoxynivalenol effect on metallothionein and glutathione levels, antioxidant capacity, and glutathione-S-transferase and liver enzymes activity in rats

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Abstract

Deoxynivalenol (DON, vomitoxin) is one of several human mycotoxins acquired through residues in foods of plant origin (cereals) and through foods of animal origin (kidneys, liver, milk, eggs). The aim of this study was to investigate the impact of deoxynivalenol-contaminated mouldy grain on the health status of rats and their ability to resist oxidative stress. The rats were fed a diet supplemented with DON along with organic and inorganic Zn(II), and vitamins for 28 days. The study focuses particularly on protective mechanisms and levels of reduced glutathione and metallothioneins (MT) against the adverse effects of xenobiotics. The highest concentrations of MT were detected in the tissues of kidneys ((6.70 ± 0.05) μg g−1) and liver ((6.00 ± 0.05) μg g−1), both of which are involved in detoxifying cationic xenobiotics. Lower concentrations were also detected in heart, brain, testes, and muscle tissues (approximately 3.0 μg g−1). Antioxidant capacity, glutathione-S-transferase, and liver enzymes activity were also studied. In conclusion, MT may play an important role in the detoxification of mycotoxins. Even though its role is not fully understood, MT are crucial for the redistribution of ions important for transcription factors and interaction with reactive oxygen species that form mycotoxins.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00, Brno, Czech Republic

    Pavlina Sobrova, Rene Kizek & Vojtech Adam

  2. Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00, Brno, Czech Republic

    Anna Vasatkova, Jiri Skladanka & Ladislav Zeman

  3. Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42, Brno, Czech Republic

    Miroslava Beklova

  4. Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00, Brno, Czech Republic

    Rene Kizek

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  1. Pavlina Sobrova
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  2. Anna Vasatkova
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  3. Jiri Skladanka
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  4. Miroslava Beklova
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  5. Ladislav Zeman
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  6. Rene Kizek
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  7. Vojtech Adam
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Correspondence to Vojtech Adam.

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Authors contributed equally to this work and may be considered co-first authors and are listed in alphabetical order.

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Sobrova, P., Vasatkova, A., Skladanka, J. et al. Study of deoxynivalenol effect on metallothionein and glutathione levels, antioxidant capacity, and glutathione-S-transferase and liver enzymes activity in rats. Chem. Pap. 66, 1092–1102 (2012). https://doi.org/10.2478/s11696-012-0229-0

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