Chemical Papers

, Volume 64, Issue 6, pp 729–733

Determination of catechin and epicatechin in the peel of apple varieties resistant and non-resistant to apple scab

  • Marek Gołębiowski
  • Edmund Maliński
  • Małgorzata Szankin
  • Mariusz Marszeniuk
  • Monika Paszkiewicz
  • Piotr Stepnowski
Original Paper

Abstract

Catechin and epicatechin were analysed in the peel of six apple cultivars (three resistant and three non-resistant to apple scab). Two methods of analytical sample preparation following extraction were tested: solid phase extraction and column separation with Sephadex LH-20 coupled to a differential refractometric detector. Prior to GC and GC-MS analyses, these compounds were silylized. This permitted co-injection with standards and the comparison of retention values and mass spectra with those recorded for standards. The content of catechin and epicatechin in apple peel is discussed in relation to the resistance of apple trees to scab.

Keywords

catechins apple scab refractometric detector GC GC-MS 

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Copyright information

© Institute of Chemistry, Slovak Academy of Sciences 2010

Authors and Affiliations

  • Marek Gołębiowski
    • 1
  • Edmund Maliński
    • 1
  • Małgorzata Szankin
    • 1
  • Mariusz Marszeniuk
    • 1
  • Monika Paszkiewicz
    • 1
  • Piotr Stepnowski
    • 1
  1. 1.Faculty of ChemistryUniversity of GdańskGdańskPoland

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