Abstract
A simple and fast flow injection fluorescence quenching method for the determination of iron in water has been developed. Fluorimetric determination is based on the measurement of the quenching effect of iron on salicylic acid fluorescence. An emission peak of salicylic acid in aqueous solution occurs at 409 nm with excitation at 299 nm. The carrier solution used was 2 × 10−6 mol L−1 salicylic acid in 0.1 mol L−1 NH +4 /NH3 buffer solution at pH 8.5. Linear calibration was obtained for 5–100 μg L−1 iron(III) and the relative standard deviation was 1.25 % (n = 5) for a 20 μL injection volume iron(III). The limit of detection was 0.3 μg L−1 and the sampling rate was 60 h−1. The effect of interferences from various metals and anions commonly present in water was also studied. The method was successfully applied to the determination of low levels of iron in real samples (river, sea, and spring waters).
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Andac, M., Asan, A., & Isildak, I. (2009). A simple flow injection spectrophotometric determination method for iron(III) based on O-acetylsalicylhydroxamic acid complexation. Chemical Papers, 63, 268–273. DOI: 10.2478/s11696-009-0024-8.
Asan, A., Andac, M., & Isildak, I. (2003). Flow-injection spectrophotometric determination of nanogram levels of iron(III) with N,N-dimethylformamide. Analytical Sciences, 19, 1033–1036. DOI: 10.2116/analsci.19.1033-1036.
Asan, A., Andac, M., Isildak, I., & Tinkilic, N. (2008). Flow injection spectrophotometric determination of iron(III) using diphenylamine-4-sulfonic acid sodium salt. Chemical Papers, 62, 345–349. DOI: 10.2478/s11696-008-0048-5.
Bagheri, H., Gholami, A., & Najafi, A. (2000). Simultaneous preconcentration and speciation of iron(II) and iron(III) in water samples by 2-mercaptobenzimidazole-silica gel sorbent and flow injection analysis system. Analytica Chimica Acta, 424, 233–242. DOI: 10.1016/S0003-2670(00)01151-X.
Cha, K.-W., & Park, C.-I. (1996). Spectrofluorimetric determination of iron(III) with 2-pyridinecarbaldhyde-5-nitropyridylhydrazone in the presence of hexadecyltrimethylammonium bromide surfactant. Talanta, 43, 1335–1341. DOI: 10.1016/0039-9140(96)01889-9.
Cha, K.-W., & Park, K.-W. (1998). Determination of iron(III) with salicylic acid by the fluorescence quenching method. Talanta, 46, 1567–1571. DOI: 10.1016/S0039-9140(98)00032-0.
Currie, L. A. (1995). Nomenclature in evaluation of analytical methods including detection and quantification capabilities (IUPAC recommendations 1995). Pure and Applied Chemistry, 67, 1699–1723.
Ragos, G. C., Demertzis, M. A., & Issopoulos, P. B. (1998). A high-sensitive spectrofluorimetric method for the determination of micromolar concentrations of iron(III) in bovine liver with 4-hydroxyquinoline. II Farmaco, 53, 611–616. DOI: 10.1016/S0014-827X(98)00070-6.
Giokas, D. L., Paleologos, E. K., & Karayannis, M. I. (2002). Speciation of Fe(II) and Fe(III) by the modified ferrozine method, FIA-spectrophotometry, and flame AAS after cloudpoint extraction. Analytical and Bioanalytical Chemistry, 373, 237–243. DOI: 10.1007/s00216-002-1326-7.
Işıldak, I., Asan, A., & Andaç, M. (1999). Spectrophotometric determination of copper(II) at low μg l−1 levels using cationexchange microcolumn in flow-injection. Talanta, 48, 219–224. DOI: 10.1016/S0039-9140(98)00241-0
Kawakubo, S., Natio, A., Fujihara, A., & Iwatsuki, M. (2004). Field determination of trace iron in fresh water samples by visual and spectrophotometric methods. Analytical Sciences, 20, 1159–1163. DOI: 10.2116/analsci.20.1159.
Ohno, S., Tanaka, M., Teshima, N., & Sakai, T. (2004). Successive determination of copper and iron by a flow injection-catalytic photometric method using a serial flow cell. Analytical Sciences, 20, 171–175. DOI: 10.2116/analsci.20.171.
Pascual-Reguera, M. I., Ortega-Carmona, I., & Molina-Díaz, A. (1997). Spectrophotometric determination of iron with ferrozine by flow-injection analysis. Talanta, 44, 1793–1801. DOI: 10.1016/S0039-9140(97)00050-7.
Pojanagaroon, T., Watanesk, S., Rattanaphani, V., & Liawrungrath, S. (2002). Reverse flow injection spectrophotometric determination of iron(III) using norfloxacin. Talanta, 58, 1293–1300. DOI: 10.1016/S0039-9140(02)00420-4.
Tamm, L. K., & Kalb, E. (1993). Microspectrofluorometry on supported planar membranes. In S. G. Schulman (Ed.), Molecular luminescence spectroscopy. Methods and applications: Part 3 (pp. 303). New York, NY, USA: Wiley.
Tarafder, P. K., & Thakur, R. (2005). Surfactant-mediated extraction of iron and its spectrophotometric determination in rocks, minerals, soils, stream sediments and water samples. Microchemical Journal, 80, 39–43. DOI: 10.1016/j.microc.2004.09.004.
Tesfaldet, Z. O., van Staden, J. F., & Stefan, R. I. (2004). Sequential injection spectrophotometric determination of iron as Fe(II) in multi-vitamin preparations using 1,10-phenanthroline as complexing agent. Talanta, 64, 1189–1195. DOI: 10.1016/j.talanta.2004.02.044.
Teshima, N., Ayukawa, K., & Kawashima, T. (1996). Simultaneous flow injection determination of iron (III) and vanadium (V) and of iron (III) and chromium (VI) based on redox reactions. Talanta, 43, 1755–1760. DOI: 10.1016/0039-9140(96)01966-2.
Themelis, D. G., Tzanavaras, P. D., Kika, F. S., & Sofoniou, M. C. (2001). Flow-injection manifold for the simultaneous spectrophotometric determination of Fe(II) and Fe(III) using 2,2′-dipyridyl-2-pyridylhydrazone and a single-line double injection approach. Fresenius’ Journal of Analytical Chemistry, 371, 364–368. DOI: 10.1007/s002160100930
Udnan, Y., Jakmunee, J., Jayasavati, S., Christian, G. D., Synovec, R. E., & Grudpan, K. (2004). Cost-effective flow injection spectrophotometric assay of iron content in pharmaceutical preparations using salicylate reagent. Talanta, 64, 1237–1240. DOI: 10.1016/j.talanta.2004.03.067.
van Staden, J. F., & Kluever, L. G. (1998). Determination of total iron in ground waters and multivitamin tablets using a solid-phase reactor with tiron immobilised on amberlite ion-exchange resin in a flow injection system. Fresenius’ Journal of Analytical Chemistry, 362, 319–323. DOI: 10.1007/s002160051081.
Weeks, D. A., & Bruland, K. W. (2002). Improved method for shipboard determination of iron in seawater by flow injection analysis. Analytica Chimica Acta, 453, 21–32. DOI: 10.1016/S0003-2670(01)01480-5.
Yan, G.-F., Shi, G.-R., & Liu, Y.-M. (1992). Fluorimetric determination of iron with 5-(4-methylphenylazo)-8-aminoquinoline in the presence of surfactants. Analytica Chimica Acta, 264, 121–124. DOI: 10.1016/0003-2670(92)85305-P.
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Asan, A., Andac, M. & Isildak, I. Flow injection spectrofluorimetric determination of iron(III) in water using salicylic acid. Chem. Pap. 64, 424–428 (2010). https://doi.org/10.2478/s11696-010-0025-7
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DOI: https://doi.org/10.2478/s11696-010-0025-7