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Immobilization of urease in poly(1-vinyl imidazole)/poly(acrylic acid) network

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Abstract

In this study, urease was immobilized in a polymer network obtained by complexation of poly(1-vinyl imidazole) (PVI) with poly(acrylic acid) (PAA). Preparation of the polymer network was monitored by FT-IR spectroscopy. Scanning electron microscopy (SEM) revealed that enzyme immobilization had a strong effect on film morphology. Proton conductivity of the PVI/PAA network was measured via impedance spectroscopy under humidified conditions. Values of the Michaelis-Menten constant (K M) for immobilized urease were higher than for the free enzyme, indicating a decreased affinity of the enzyme to its substrate. The basic characteristics (pHopt, pHstability, T opt, T stability, reusability, and storage stability) of immobilized urease were determined. The results show that the PAA/PVI polymer network is suitable for enzyme immobilization.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Arts and Sciences, Fatih University, B. Cekmece, Istanbul, 34500, Turkey

    Mehmet Şenel & Ayhan Bozkurt

  2. Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, B. Cekmece, Istanbul, 34500, Turkey

    Agah Coşkun & M. Fatih Abasıyanık

Authors
  1. Mehmet Şenel
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  2. Agah Coşkun
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  3. M. Fatih Abasıyanık
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  4. Ayhan Bozkurt
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Correspondence to Mehmet Şenel.

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Şenel, M., Coşkun, A., Fatih Abasıyanık, M. et al. Immobilization of urease in poly(1-vinyl imidazole)/poly(acrylic acid) network. Chem. Pap. 64, 1–7 (2010). https://doi.org/10.2478/s11696-009-0103-x

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