Skip to main content
SpringerLink
Log in

Improved DPPH determination for antioxidant activity spectrophotometric assay

  • Short Communication
  • Published:
Chemical Papers Aims and scope Submit manuscript

Abstract

An improved procedure for determination of the residual DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical concentration was proposed taking into account the absorbance of both DPPH free radicals and DPPH nonradical (1,1-diphenyl-2-picrylhydrazine) stable form. The calculated residual DPPH free radical concentrations were compared with those obtained from a calibration curve and variation coefficients below 10 % were found.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Pourmorad, F., Hosseinimehr, S. J., and Shahabimajd, N., Afr. J. Biotechnol. 5, 1142 (2006).

    CAS  Google Scholar 

  2. Sun, Y. M., Wang, R. X., Yuan, S. L., Lin, X. J., and Liu, C. B., Chin. J. Chem. 22, 827 (2004).

    Article  CAS  Google Scholar 

  3. Cook, N. C. and Samman, S., J. Nutr. Biochem. 7, 66 (1996).

    Article  CAS  Google Scholar 

  4. Halliwell, B., Aeschbach, R., Loliger, J., and Aruoma, O. I., Food Chem. Toxicol. 33, 601 (1995).

    Article  PubMed  CAS  Google Scholar 

  5. Aruoma, O. I., Free Radical Biol. Med. 20, 675 (1996).

    Article  CAS  Google Scholar 

  6. Huang, D. J., Chen, H. J., Lin, C. D., and Lin, Y. H., Bot. Bull. Acad. Sin. 46, 99 (2005).

    Google Scholar 

  7. Halliwell, B., Biochem. Pharmacol. 49, 1341 (1995).

    Article  PubMed  CAS  Google Scholar 

  8. Vaya, J. and Aviram, M., Curr. Med. Chem.-Immunol. Endocr. Metab. Agents 1, 99 (2001).

    Article  CAS  Google Scholar 

  9. Amić, D., Davidović, D., Bešlo, D., and Trinajstić, N., Croat. Chem. Acta 76, 55 (2003).

    Google Scholar 

  10. Middleton, E., Kandaswami, C., and Theoharides, C., Pharmacol. Rev. 52, 673 (2000).

    PubMed  CAS  Google Scholar 

  11. Benzie, I. F. F. and Strain, J. J., Anal. Biochem. 239, 70 (1996).

    Article  PubMed  CAS  Google Scholar 

  12. Dapkevicius, A., Venskutonis, R., Beek, T. A., and Linssen, J. P. H., J. Sci. Food Agric. 77, 140 (1998).

    Article  CAS  Google Scholar 

  13. Dawidowicz, A. L., Wianowska, D., and Baraniak, B., LWT-Food Sci. Technol. 39, 308 (2006).

    Article  CAS  Google Scholar 

  14. Silva, F. A. M., Borges, M. F. M., and Ferreira, M. A., ECSOC-2, [dp259], 1998; http://www.mdpi.org/ecsoc/

  15. Proestos, C., Boziaris, I. S., Nychas, G. J. E., and Komaitis, M., Food Chem. 95, 664 (2006).

    Article  CAS  Google Scholar 

  16. Li, Y. F., Guo, C. J., Yang, J. J., Wei, J. Y., Xu, J., and Cheng, S., Food Chem. 96, 254 (2006).

    Article  CAS  Google Scholar 

  17. Tepe, B., Sokmen, M., Akpulat, H. A., and Sokmen, A., Food Chem. 95, 200 (2006).

    Article  CAS  Google Scholar 

  18. Katalinic, V., Milos, M., Kulisic, T., and Jukic, M., Food Chem. 94, 550 (2006).

    Article  CAS  Google Scholar 

  19. Gong, Y., Toivonen, P. M. A., Lau, O. L., and Wiersma, P. A., Bot. Bull. Acad. Sin. 42, 259 (2001).

    CAS  Google Scholar 

  20. Thippeswamy, N. B. and Naidu, K. A., Eur. Food Res. Technol. 220, 472 (2005).

    Article  CAS  Google Scholar 

  21. Molyneux, P., Songklanakarin J. Sci. Technol. 26, 211 (2004).

    CAS  Google Scholar 

  22. Reschiglian, P., Zattoni, A., Melucci, D., Locatelli, C., Torsi, G., and Pandalai, S. G., Recent Research Developments in Applied Spectroscopy, Vol. 3, p. 61. Research Signpost, Trivandrum, INDE, 2000.

    Google Scholar 

  23. Scapim, C. A., Carvalho, C. G. P., and Cruz, C. D., Pesquisa Agr. Bras. 30, 683 (1995).

    Google Scholar 

  24. Blois, M. S., Nature 181, 1199 (1958).

    Article  ADS  CAS  Google Scholar 

  25. Gomez-Alonso, S., Fregapane, G., Salvador, M. D., and Gordon, M. H., J. Agric. Food Chem. 51, 667 (2003).

    Article  PubMed  CAS  Google Scholar 

  26. Lebeau, J., Furman, C., Bernier, J. L., Duriez, P., Teissier, E., and Cotelle, N., Free Radical Biol. Med. 29, 900 (2000).

    Article  CAS  Google Scholar 

  27. Yepez, B., Espinosa, M., Lopez, S., and Bolanos, G., Fluid Phase Equilib. 194, 879 (2002).

    Article  Google Scholar 

  28. Bondet, V., Brand-Williams, W., and Berset, C., Food Sci. Technol. 30, 609 (1997).

    CAS  Google Scholar 

  29. Schwarz, K., Bertelsen, G., Nissen, L. R., Gardner, P. T., Heinonen, M. I., Hopia, A., Huynh-Ba, T., Lambelet, P., McPhail, D., Skibsted, L. H., and Tijburg, L., Eur. Food Res. Technol. 212, 319 (2001).

    Article  CAS  Google Scholar 

  30. Mosquera, O. M. M., Nino, J. O., Correa, Y. M., and Buitrago-Bejarano, D. C., Sci. Tech. 27, 231 (2005).

    Google Scholar 

  31. Lee, K. W., Kim, Y. J., Lee, H. J., and Lee, C. Y., J. Agric. Food Chem. 51, 7292 (2003).

    Article  PubMed  CAS  Google Scholar 

  32. Leitao, G. G., Laitao, S. G., and Vilegas, W., Z. Naturforsch., C: Biosci. 57, 1051 (2002).

    CAS  Google Scholar 

  33. Kim, J.-K., Noh, J. H., Lee, S., Choi, J. S., Suh, H., Chung, H. Y., Song, Y.-O., and Choi, W. C., Bull. Korean Chem. Soc. 23, 661 (2002).

    Article  CAS  Google Scholar 

  34. Szabo, M. R., Chambre, D., and Idioiu, C., Scientific and Technical Bulletin of “Aurel Vlaicu” University, Series: Chemistry, Food Science, and Engineering 11, 122 (2006): www.uav.ro

Download references

Author information

Authors and Affiliations

  1. Chemical and Technological Research Centre, Food Engineering, Tourism, and Environmental Protection Faculty, “Aurel Vlaicu” University, Elena Dragoi Street 2, Arad, 310330, Romania

    M. R. Szabo, C. Idiţoiu & D. Chambre

  2. Chemistry and Organic Technologies Department, “Politehnica” University from Timişoara, FCIIM, Telbisz Street 6, Timişoara, 300001, Romania

    A. X. Lupea

Authors
  1. M. R. Szabo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. Idiţoiu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. Chambre
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. X. Lupea
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Szabo, M.R., Idiţoiu, C., Chambre, D. et al. Improved DPPH determination for antioxidant activity spectrophotometric assay. Chem. Pap. 61, 214–216 (2007). https://doi.org/10.2478/s11696-007-0022-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.2478/s11696-007-0022-7

Keywords

Search

Navigation