Summary
Longidorus caespiticola was found for the first time from the rhizosphere of apple with two other Longidorus sp. at Žlunice, Czech Republic. Females and males were analyzed morphologically and morphometrically. Four regions (18S, ITS1, ITS2, D2-D3 expansion segments of 28S) of ribosomal DNA and one region (cox1) of mitochondrial DNA were sequenced and analyzed and compared with a population from the Slovak Republic. The partial mitochondrial cytochrome c oxidase subunit 1 (cox1) gene showed relatively high genetic variation compared with ribosomal DNA between the Czech and Slovakian populations.
Similar content being viewed by others
References
Andres, M. F., Arias, M., Bello, A. (1991): Distribution and ecology of Longidorus (Micoletzky) Filipjev in the central region of Spain. Nematropica, 21: 79–87
Chen, Q. W., Hooper, D. J., Loof, P. A. A., Xu, J. (1997): A revised polytomous key for the identification of species of the genus Longidorus Micoletzky, 1922 (Nematoda: Dorylaimoidea). Fundam. appl. Nematol., 20: 15–28
Cherry, T., Szalanski, A. L., Todd, T. C., Powers, T. O. (1997): The Internal Transcribed Spacer Region of Belonolaimus (Nemata: Belonolaimidae). J. Nematol., 29 (1): 23–29
Coomans, A., De Ley, I. T., Jimenez, L. A., De Ley, P. (2012): Morphological, molecular characterisation and phylogenetic position of Longidorus mindanaoensis n. sp. (Nematoda: Longidoridae) from a Philippine Avicennia mangrove habitat. Nematology, 14: 285–307. DOI: 10.1163/156854111X594974
De Ley, P., Félix, M. A., Frisse, L. M., Nadler, S. A., Sternberg, P. W., Thomas, W. K. (1999): Molecular and morphological characterisation of two reproductively isolated species with mirror-image anatomy (Nematoda: Cephalobidae). Nematology, 1: 591–612. DOI: 10.1163/156854199508559
De Waele, D., Coomans, A. (1990): Occurrence and ecology of longidorid nematodes in Belgium. Rev. Nématol., 13: 197–202
Erbenová, M. (1977): Nematodes in the root zone of plum orchards. Věd. práce ovoc., 6: 361–370
He, Y., Jones, J., Armstrong, M., Lamberti, F., Moens, M. (2005a): The mitochondrial genome of Xiphinema americanum sensu stricto (Nematoda: Enoplea): Considerable economization in the length and structural features of encoded genes. J. Mol. Evol., 61: 819–833. DOI: 10.1007/s00239-005-0102-7
He, Y., Subbotin, S. A., Rubtsova, T. V., Lamberti, F., Brown, D. J. F., Moens, M. (2005b): A molecular phylogenetic approach to Longidoridae (Nematoda: Dorylaimida). Nematology, 7: 111–124. DOI: 10.1163/1568541054192108
Holterman, M., Wurff, A. V. D., Elsen, S. V. D., Megen, H. V., Bongers, T., Holovachov, O. (2006): Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades. Mol. Biol. Evol., 23: 1792–1800. DOI: 10.1093/molbev/mls044
Hoof, H. A. V. (1966): Nematode populations active and inactive with regard to transmission of nepo viruses. Nematologica, 12: 615–618
Hooper, D. J. (1961): A redescription of Longidorus elongates (De Man, 1876) Thorne & Swanger, 1936, (Nematoda, Dorylaimidae) and descriptions of five new species of Longidorus from Great Britain. Nematologica, 6: 237–257
Kumari, S., Decraemer, W. (2007): The genus Longidorus (Nematoda: Longidoridae) from Bohemia and South Moravia in the rhizosphere of fruit orchards and vineyards. Helminthologia, 44: 193–203. DOI: 10.2478/s11687-007-0031-7
Kumari, S., Decraemer, W., Traversa, D., Lišková, M. (2009): Molecular and morphological delineation of Longidorus poessneckensis Altherr, 1974 (Nematoda: Dorylaimida). Eur. J. Pl. Path., 123: 125–137. DOI: 10.1007/s10658-008-9348-4
Kumari, S., Subbotin, S. A. (2012): Characterization of Longidorus helveticus (Nematoda: Longidoridae) from the Czech Republic. Eur. J. Pl. Path., 133: 923–933. DOI: 10.1007/s10658-012-9959-7
Lazarova, S. S., Malloch, G., Oliveira, C. M. G., Hübschen, J., Neilson, R. (2006): Ribosomal and mitochondrial DNA analyses of Xiphinema americanum-group populations. J. Nematol., 38: 404–410
Lišková, M., Brown, D. J. F. (1998): Longidoridae (Nematoda) associated with walnut trees (Juglans regia L.) in Slovak Republic. Helminthologia, 35: 93–99
Morgan, J. A. T., Blair, D. (1998). Relative merits of nuclear ribosomal internal transcribed spacers and mitochondrial CO1 and ND1 genes for distinguishing among Echinostoma species (Trematoda). Parasitology, 116: 289–297. DOI: 10.1017/S0031182097002217
Peneva, V. K., Urek, G., Lazarova, S., Širca, S., Knapič, M., Elshishka, M., Brown, D. J. F. (2012): Longidoridae and nepoviruses in Bulgaria and Slovenia. Helminthologia, 49: 49–56. DOI: 10.2478/s11687-012-0008-z
Rubtsova, T. V., Subbotin, S. A., Brown, D. J. F., Moens, M. (2001): Description of Longidorus sturhani sp. n. (Nematoda: Longidoridae) and molecular characterization of several longidorid species from Western Europe. Rus. J. Nematol., 9: 127–136
Širca, S, Urek, G. (2009): Morphological and molecular characterization of six Longidorus species (Nematoda: Longidoridae) from Slovenia. Rus. J. Nematol., 17: 95–105
Stanton, J. M., Mcnicol, C. D., Steele, V. (1998): Nonmanual lysis of second-stage Meloidogyne juveniles for identification of pure and mixed samples based on the polymerase chain reaction. Australas. Plant Pathol., 27: 112–115. DOI: 10.1071/AP98014.
Sturhan, D. (1963): A contribution to the systematics of Longidorus. Nematologica, 9: 131–142
Subbotin, S. A., Rogozhin, E. A., Chizhov, V. N. (2014): Molecular characterisation and diagnostics of some Longidorus species (Nematoda: Longidoridae) from Russia and other countries using rRNA genes. Eur. J. Pl. Path., 138: 377–390. DOI: 10.1007/s10658-013-0338-9
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S. (2011): MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol., 28: 2731–2739. DOI: 10.1093/molbev/msr121
Taylor, C. E., Brown, D. J. F (1976): The geographical distribution of Xiphinema and Longidorus nematodes in the British Isles and Ireland. Ann. appl. Biol., 84: 383–402
Taylor, C. E., Brown, D. J. F. (1997): Nematode vectors of plant viruses. CABI, UK
Vrain, T. C., Wakarchuk, D. A., Levesque, A. C., Hamilton, R. I. (1992): Intraspecific rDNA restriction fragment length polymorphism in the Xiphinema americanum group. Fundam. appl. Nematol., 15: 563–573
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Kumari, S. Characterization of Longidorus caespiticola (Nematoda: Longidoridae) from the Czech Republic. Helminthologia 51, 153–157 (2014). https://doi.org/10.2478/s11687-014-0222-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.2478/s11687-014-0222-y