Skip to main content
Log in

Molecular confirmation of Xiphinema italiae Meyl, 1953 (Nematoda: Longidoridae) from the Slovak Republic

  • Scientific Article
  • Published:
Helminthologia

Summary

Identification of the nematode Xiphinema italiae relies mainly on time-consuming morphological and morphometrical studies. A polymerase chain reaction protocol has been used for the reliable and specific identification of X. italiae. Moreover, four independently evolving molecular markers (cox1- cytochrome c oxidase subunit 1; ITS2-second internal transcribed spacer; 18S gene and D2/D3 expansion segments of 28S gene) were amplified and sequenced in both directions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Reference

  • Barsi, L., Lamberti, F. (2003): Morphometrics of adults and juvenile stages of three longidorid nematodes (Nematoda: Dorylaimida) from Vojvodina Province, Northern Serbia. Nematol. Medit., 31: 65–85

    Google Scholar 

  • Brown, D. J. F., Boag, B. (1988): An examination of methods used to extract virus-vector nematodes (Nematoda: Longidoridae and Trichodoridae) from soil samples. Nematol. Medit., 16: 93–99

    Google Scholar 

  • Cohn, E., Tanne, E., Nitzany, F. E. (1970): Xiphinema italiae, a new vector of grapevine fanleaf virus. Phytopathology, 60: 181–182

    Article  Google Scholar 

  • de Ley, P., Félix, M. A., Frisse, L. M., Nadler, S. A., Sternberg, P. W., Thomas, W. K. (1999): Molecular and morphological characterisation of two reproductively isolated species with mirror-image anatomy (Nematoda: Cephalobidae). Nematology, 1: 591–612

    Article  Google Scholar 

  • Derycke, S., Remerie, T., Vierstraete, A., Backeljau, T., Vanfleteren, J., Vincx, M., Moens, T. (2005): Mitochondrial DNA variation and cryptic speciation within the free-living marine nematode Pellioditis marina. Mar. Ecol. Prog. Ser., 300: 91–103

    Article  CAS  Google Scholar 

  • Dias-Silveira, M.F., Herrera, J.O. (1995): Principales problemas nematologicos en Cuba. In: Congresso International de Nematologia Tropical, Rio Quente, Brazil. Proceedings, 161–171

  • He, Y., Jones, J., Armstrong, M., Lamberti, F., Moens, M. (2005a): The mitochondrial genome of Xiphinema americanum sensu stricto (Nematoda: Enoplea): Considerable economization in the length and structural features of encoded genes. J. Mol. Evol., 61: 819–833

    Article  PubMed  CAS  Google Scholar 

  • He, Y., Subbotin, S. A., Rubtsova, T. V., Lamberti, F., Brown, D. J. F., Moens, M. (2005b): A molecular phylogenetic approach to Longidoridae (Nematoda: Dorylaimida). Nematology, 7: 111–124

    Article  CAS  Google Scholar 

  • Holterman, M., Wurff, A. V. D., Elsen, S. V. D., Megen, H. V., Bongers, T., Holovachov, O., Bakker, J., Helder, J. (2006): Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades. Mol. Biol. Evol., 23: 1792–1800

    Article  PubMed  CAS  Google Scholar 

  • Khan, F. A., Erinle, I. D., Chindo, P. S. (1993): Survey of plant parasitic nematodes associated with grapevine in 4 northern states of Nigeria and observations on grapevine fanleaf virus. J. Afr. Zool., 107: 505–510

    Google Scholar 

  • Knoetze, R., Burger, J. T, Meyer, A. J. (2000): Discrimination of some Xiphinema species from South Africa by rDNA-RFLP analysis. Afr Plant Protect., 6: 25–30

    Google Scholar 

  • Kumari, S., Decraemer, W., Traversa, D., Lišková, M. (2009): Molecular and morphological delineation of Longidorus poessneckensis Altherr, 1974 (Nematoda: Dorylaimida). Eur. J. Pl. Path., 123: 125–137

    Article  CAS  Google Scholar 

  • Lamberti, F., Agostinelli, A., Radicci, V. (1996): Longidorid nematodes from Northern Egypt. Nematol. Medit., 24: 307–339

    Google Scholar 

  • Lazarova, S. S., Malloch, G., Oliveira, C. M. G., Hübschen, J., Neilson, R. (2006): Ribosomal and mitochondrial DNA analyses of Xiphinema americanum-group populations. J. Nematol., 38: 404–410

    PubMed  CAS  Google Scholar 

  • Lišková, M. (1996): The males of Longidorus leptocephalus and Xiphinema italiae (Nematoda: Longidoridae) from Slovakia. Helminthologia, 33: 87–92

    Google Scholar 

  • Liskova, M., Brown, D. J. F., Taylor, C. E. (1995): The occurrence and distribution of Longidoridae and Trichodoridae in the Slovak Republic. Rus. J. Nematol., 3: 49–60

    Google Scholar 

  • Liskova, M., Lamberti, F., Sabová, M., Valocká, B., Agostinelli, A. (1993): First record of some species of longidorid nematodes from Slovakia. Nematol. Medit., 21: 49–53

    Google Scholar 

  • Martelli, G. P., Cohn, E., Dalmasso, A. (1966): A redescription of Xiphinema italiae Meyl, 1953 and its relationship to Xiphinema arenarium Luc et Dalmasso, 1963 and Xiphinema conurum Siddiqi, 1964. Nematologica, 12: 183–194

    Article  Google Scholar 

  • Nagy, P. (1999): New faunistic record for Longidorus attenuatus Hooper, 1961 and Xiphinema italiae Meyl, 1953 (Nematoda: Longidoridae) in Hungary. Növényvédelem, 35: 15

    Google Scholar 

  • Oliveira, C. M. G., Fenton, B., Malloch, G., Brown, D. J. F., Neilson, R. (2005): Development of species-specific primers for the ectoparasitic nematode species Xiphinema brevicolle, X. diffusum, X. elongatum, X. ifacolum and X. longicaudatum (Nematoda: Longidoridae) based on ribosomal DNA sequences. Ann. Appl. Biol., 146: 281–288

    Article  CAS  Google Scholar 

  • Peneva, V., Choleva, B. (1992): Nematodes of the family Longidoridae from forest nurseries in Bulgaria. II. The genus Xiphinema Cobb, 1913. Helminthology, 32: 46–58

    Google Scholar 

  • Polinovskij, A. I. (1979): Species composition and geographic distribution of nematodes of the family Longidoridae (Nematoda: Dorylaimoidea) on grapevines of the Moldavian SSR, USSS. Izv. Akad. Nauk Mold. SSR. Ser. Biol. chim. Nauk, 11: 37–48

    Google Scholar 

  • Romanenko, N. D. (1985): Distribution of Longidoridae nematodes in fruit and berry plantations in the USSR. In: Progressive methods of soft fruit cultivation and breeding. Sbornik Nauch. Trud. Moscov, 112–120

  • Siddiqui, Z. A., Rashid, A., Farooqi, N., Bisheya, F. (1987): A survey of plant parasitic nematodes associated with citrus in Libya and trials on chemical control. Indian J. Nematol., 17: 76–80

    Google Scholar 

  • Stanton, J. M., Mcnicol, C. D., Steele, V. (1998): Non-manual lysis of second-stage Meloidogyne juveniles for identification of pure and mixed samples based on the polymerase chain reaction. Australas. Plant Path., 27: 112–115

    Article  Google Scholar 

  • Teliz, D., Landa, B. B., Rapoport, H. E., Camacho, F. P., Diaz, J. R. M., Castillo, P. (2007): Plant parasitic nematodes infecting grapevine in southern Spain and susceptible reaction to root-knot nematodes of rootstocks reported as moderately resistant. Plant Dis., 91: 1147–1157

    Article  CAS  Google Scholar 

  • Tzortzakakis, E. A., Pateras, D., Charoulis, A. (2006): Occurrence of Xiphinema species in grapevine areas of Tyranvos with comments on the distribution of X. italiae in Greece. Helminthologia, 43: 186–187

    Article  Google Scholar 

  • Wang, X., Bosselut, N., Castagnone, C., Voisin, R., Abad, P., Esmenjaud, D. (2003): Multiplex polymerase chain reaction identification of single individuals of the longidorid nematodes Xiphinema index, X. diversicaudatum, X. vuittenezi, and X. italiae using specific primers from ribosomal genes. Phytopathology, 93: 160–166

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to S. Kumari.

About this article

Cite this article

Kumari, S., Lišková, M. Molecular confirmation of Xiphinema italiae Meyl, 1953 (Nematoda: Longidoridae) from the Slovak Republic. Helminthologia 46, 131–134 (2009). https://doi.org/10.2478/s11687-009-0025-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2478/s11687-009-0025-8

Keywords

Navigation