Summary
A modified McMaster method has been used for the diagnosis and estimating helminth egg load in human faecal samples obtained from random consecutive patients in the areas non-endemic for helminth infections (Slovak Republic, North West Russia). Both positive and negative findings were in a 100 % concordance to those obtained with a reference method accepted in clinical diagnostic laboratories (microscopy of the native stool smear). The McMaster method was efficient in detecting nematode eggs in patients’ stool samples with egg load varying from very low (15–60 epg for T. trichiura) to moderate (1650–4500 epg for A. lumbricoides). Therefore, this method may be successfully (and with a better technical feasibility) used for the diagnosis of intestinal helminth infections in non-endemic areas, with further quantitative analysis of the sample when required.
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References
Bekele, T. (2002): Epidemiological studies on gastrointestinal helminths of dromedary (Camelus dromedarius) in semi-arid lands of eastern Ethiopia. Vet. Parasitol., 105(2): 139–152
Bell, W. J., Nassif, S. (1971): Comparison of pyrantel pamoate and piperazine phosphate in the treatment of ascariasis. Am. J. Trop. Med. Hyg., 20(4): 584–588
Cebra, C. K., Stang, B. V. (2008): Comparison of methods to detect gastrointestinal parasites in llamas and alpacas. J. Am. Vet. Med. Assoc., 232(5): 733–741
Coles, G. C., Bauer, C. Borgsteede, F. H. M., Geerts, S., Klei, T. R., Taylor, M. A., Waller, P. J. (1992): World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Vet. Parasitol., 44(1): 35–44
Cringoli, G., Rinaldi, L., V. Veneziano, V., Capelli, G., Scala, A. (2004): The influence of flotation solution, sample dilution and the choice of McMaster slide area (volume) on the reliability of the McMaster technique in estimating the faecal egg counts of gastrointestinal strongyles and Dicrocoelium dendriticum in sheep. Vet. Parasitol., 123(1): 121–131
Dunn, A., Keymer, A. (1986): Factors affecting the reliability of the McMaster technique. J. Helminthol., 60(4): 260–262
Flohr, C., Tuyen, L. N., Lewis, S., Tan Minh, T., Campbell, J., Britton, J., Williams, H., Tinh Hien, T., Farrar, J., Quinnel, R. J. (2007): Low efficacy of mebendazole against hookworm in Vietnam: two randomized controlled trials. Am. J. Trop. Med. Hyg., 76(4): 732–736
Giannetto, S., Brianti, E., Poglayen, G., Sorgi, C., Capelli, G., Pennisi, M. G., Coci, G. (2007): Efficacy of oxfendazole and fenbendazole against tortoise (Testudo hermanni) oxyurids. Parasitol. Res., 100(5): 1069–1073
Groth, T. (1990): Some methodological aspects on quality assurance in laboratory medicine. Upsala J. Med. Sci., 95(3): 265–273
Rinaldi, L., Russo, T., Schioppi, M., Pennacchio, S., Cringoli, G. (2007): Passalurus ambiguus: new insights into copromicroscopic diagnosis and circadian rhythm of egg excretion. Parasitol. Res., 101(2): 557–561
Marshall, W. J. (2000): Clinical Chemistry: Fourth edition. Mosby Publ., Edinburgh et al.
Meyer-Lucht, Y., Sommer, S. (2005): MHC diversity and the association to nematode parasitism in the yellow-necked mouse (Apodemus flavicollis). Mol. Ecol., 14(7): 2233–2243
Sargent, R. G., Dotterer, T. D., Savory, A. M., Lee, P. R. (1975): A clinical evaluation of the efficacy of mebendazole in the treatment of trichuriasis. South. Med. J., 68(1): 38–40
Schad, S. J., Ganzhorn, J. U., Sommer, S. (2004): Parasite burden and constitution of major histocompatibility complex in the Malagasy mouse lemur, Microcebus murinus. Evolution, 59(2): 439–450
Seivwright, L. J., Redpath, S. M., Mougeot, F., Watt, L., Hudson, P. J. (2004): Faecal egg counts provide a reliable measure of Trichostrongylus tenuis intensities in free-living red grouse Lagopus lagopus scoticus. J. Helminthol., 78(1): 69–76
Stephenson, L. S., Latham, M. C., Kurz, K. M., Kinoti, S. N., Brigham, H. (1989): Treatment with a single dose of albendazole improves growth of Kenyan schoolchildren with hookworm, Trichuris trichiura, and Ascaris lumbricoides infections. Am. J. Trop. Med. Hyg., 41(1): 78–87
Thienpont, D., Brugmans, J., Abadi, K., Tanamal, S. (1969): Tetramizole in the treatment of nematode inections in man. Am. J. Trop. Med. Hyg., 18(4): 520–525
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Bondarenko, I.G., Kinčeková, J., Várady, M. et al. Use of modified McMaster method for the diagnosis of intestinal helminth infections and estimating parasitic egg load in human faecal samples in non-endemic areas. Helminthologia 46, 62–64 (2009). https://doi.org/10.2478/s11687-009-0012-0
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DOI: https://doi.org/10.2478/s11687-009-0012-0