First Molecular Characterization of Trichostrongylus colubriformis Infection in Rural Patients from Chile



The aim was to characterize the infection by Trichostrongylus spp. in patients from Chile using a combination of molecular detection techniques and phylogenetic analysis relating the findings to clinical and epidemiological reports of the patients


Strongylid eggs were detected in seven patients by coproparasitological techniques. From each sample a fragment of the ITS-2 ribosomal gene was amplified by PCR, sequenced and analyzed by the Neighbor-Joining method.


All the sequences and phylogenetic clusters corresponded to T. colubriformis. Two samples presented a single nucleotide polymorphism showing two possible haplotypes. Six patients presented gastrointestinal symptoms. All of them lived on farms and used sheep manure as fertilizer.


T. colubriformis was the strongylid involved in the infections of these Chilean patients associated with the presence of livestock and agricultural practices that favor infection by this type of nematode.

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The authors thank Dr. Raúl Sánchez Gutiérrez (from Center of Translational Medicine Scientific and Technological Nucleus (CEMT-BIOREN), Universidad de La Frontera. Temuco, Chile) for the support granted to carry out this research


This study was supported by DIUFRO (Grant number: DI 160045) from Universidad de La Frontera, Temuco, Chile.

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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by AH, PG, AM, CO, CH and FFS. The first draft of the manuscript was written by AH and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Flery Fonseca-Salamanca.

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The authors declare that they have no conflict of interest.

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Approval was obtained from the ethics committee of the Universidad de La Frontera. The procedures used in this study adhere to the tenets of the Declaration of Helsinki.

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Hidalgo, A., Gacitúa, P., Melo, A. et al. First Molecular Characterization of Trichostrongylus colubriformis Infection in Rural Patients from Chile. Acta Parasit. 65, 790–795 (2020).

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  • Trichostrongylus colubriformis
  • PCR
  • Strongylid
  • Livestock
  • Manure