The Molecular Phylogeny of Pararhadinorhynchus magnus Ha, Amin, Ngo, Heckmann, 2018 (Acanthocephala: Rhadinorhynchidae) from Scatophagus argus (Linn.) (Scatophagidae) in Vietnam



The molecular profile of Pararhadinorhynchus magnus Ha, Amin, Ngo, Heckmann, 2018 described from Scatophagus argus (Linn.) off Haiphong in the Gulf of Tonkin, Pacific Ocean, Vietnam is provided for the first time. It was morphologically distinguished from the South Australian species, Pararhadinorhynchus mugilis Johnston and Edmonds, 1947 and Pararhadinorhynchus coorongensis Edmonds, 1973 from mullets. Two other species of Pararhadinorhynchus are also recognized: Pararhadinorhynchus upenei Wang, Wang, Wu, 1993 from China and Pararhadinorhynchus sodwanensis Lisitsyna, Kudlai, Cribb and Smit, 2019 from South Africa. The assignment of Diplosentis manteri Gupta and Fatma, 1980 to Pararhadinorhynchus is not recognized.


Sequences of the 18S, small internal transcribed spacers (ITS1-5.8S-ITS2) and 28S from nuclear DNA were generated to molecularly characterize P. magnus. The phylogenetic analyses were achieved by comparison of the 18S and ITS1-5.8S-ITS2 region only as the 28S amplified a short region (425–428 bp) that was not sufficient for the present study.


Phylogenetic analyses showed that P. magnus and the other species of Pararhadinorhynchus sequenced were nested within separate clades in the case of 18S gene and suggesting that these species do not share a common ancestor. In contrast, the ITS1-5.8S-ITS2 region shows a close arrangement of species of Pararhadinorhynchus with molecular affinities to the family Diplosentidae, suggesting that final placement of these species in Transvenidae needs further study and revision.


The molecular data from the present study will provide further comparative insights into species of Pararhadinorhynchus and its close affiliation to other acanthocephalan species and genera from different geographical areas.

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  1. 1.

    Amin OM (2013) Classification of the Acanthocephala. Folia Parasitol 60:273–305

    Article  Google Scholar 

  2. 2.

    Amin OM, Chaudhary A, Heckmann R, Ha NV, Singh HS (2019) Redescription and molecular analysis of Neoechinorhynchus (Neoechinorhynchus) johnii Yamaguti, 1939 (Acanthocephala, Neoechinorhynchidae) from the Pacific Ocean off Vietnam. Parasite 26:43.

    Article  PubMed  PubMed Central  Google Scholar 

  3. 3.

    Amin OM, Heckman RA, Ali AH (2018) The finding of Pacific transvenid acanthocephalans in the Arabian Gulf with the description of Paratrajectura longcementglandatus n. gen., n. sp. from perciform fishes and emendation of Transvenidae. J Parasitol 104:39–50.

    Article  PubMed  Google Scholar 

  4. 4.

    Amin OM, Heckman RA, Dallarés S, Constenla M, Ha NV (2019) Morphological and molecular description of Rhadinorhynchus laterospinosus Amin, Heckmann & Ha, 2011 (Acanthocephala, Rhadinorhynchidae) from marine fish off the Pacific coast of Vietnam. Parasite 26:14.

    Article  PubMed  PubMed Central  Google Scholar 

  5. 5.

    Amin OM, Heckman RA, Dallarés S, Constenla M, Ha NV (2020) Morphological and molecular description of Rhadinorhynchus hiansi Soota and Bhattacharya, 1981 (Acanthocephala: Rhadinorhynchidae) from marine fish off the Pacific coast of Vietnam. J Parasitol 106:56–70

    CAS  Article  Google Scholar 

  6. 6.

    Arizono N, Kuramochi T, Kagei N (2012) Molecular and histological identification of the acanthocephalan Bolbosoma cf. capitatum from the human small intestine. Parasitol Int 61:715–718.

    CAS  Article  PubMed  Google Scholar 

  7. 7.

    Braicovich PE, Lanfranchi AL, Farber MD, Marvaldi AE, Luque JL, Timi JT (2014) Genetic and morphological evidence reveals the existence of a new family, genus and species of Echinorhynchida (Acanthocephala). Folia Parasitol 61:377–384

    Article  Google Scholar 

  8. 8.

    Edmonds SJ (1973) Australian Acanthocephala No. 14. On two species of Pararhadinorhynchus, one new. Trans R Soc Aust 97:19–21

    Google Scholar 

  9. 9.

    Fonseca MCGD, Knoff M, Felizardo NN, Torres EJL, Di Azevedo MIN, Gomes DC, Clemente SCS, Iñiguez AM (2019) Acanthocephalan parasites of the flounder species Paralichthys isosceles, Paralichthy spatagonicus and Xystreurys rasile from Brazil. Rev Bras Parasitol Vet 28:346–359.

    CAS  Article  PubMed  Google Scholar 

  10. 10.

    Galazzo DE, Dayanandan S, Marcogliese DJ, McLaughlin JD (2002) Molecular systematics of some North American species of Diplostomum (Digenea) based on rDNA-sequence data and comparisons with European congeners. Can J Zool 80:2207–2217.

    CAS  Article  Google Scholar 

  11. 11.

    García-Varela M, Aznar FJ, Nadler SA (2009) Systematic position of Pseudocorynosoma and Andracantha (Acanthocephala, Polymorphidae) based on nuclear and mitochondrial gene sequences. J Parasitol 95:178–185.

    Article  PubMed  Google Scholar 

  12. 12.

    García-Varela M, Pérez-Ponce de León G, Aznar FJ, Nadler SA (2011) Ibirhynchus dimorpha n. gen. (Acanthocephala: Polymorphidae), inferred through morphological, ecological and molecular data. J Parasitol 97:97–105.

    Article  PubMed  Google Scholar 

  13. 13.

    García-Varela M, Pérez-Ponce de Leon G, de la Torre P, Cummings MP, Sarma SS, Laclette JP (2000) Phylogenetic relationships of Acanthocephala based on analysis of 18S ribosomal RNA gene sequences. J Mol Evol 50:532–540.

    Article  PubMed  Google Scholar 

  14. 14.

    García-Varela M, Mendoza-Garfias B, Choudhury A, Pérez-Ponce de León G (2017) Morphological and molecular data for a new species of Pomphorhynchus Monticelli, 1905 (Acanthocephala: Pomphorhynchidae) in the Mexican redhorse Moxostoma austrinum Bean (Cypriniformes: Catostomidae) in central Mexico. Syst Parasitol 94:989–1006.

    Article  PubMed  Google Scholar 

  15. 15.

    García-Varela M, Nadler SA (2005) Phylogenetic relationships of Palaeacantocephala (Acanthocephala) inferred from SSU and LSU rDNA gene sequences. J Parasitol 91:1401–1409.

    Article  PubMed  Google Scholar 

  16. 16.

    García-Varela M, Pérez-Ponce de León G, Aznar FJ, Nadler SA (2013) Phylogenetic relationship among genera of Polymorphidae (Acanthocephala), inferred from nuclear and mitochondrial gene sequences. Mol Phylogenet Evol 68:176–184.

    Article  PubMed  Google Scholar 

  17. 17.

    Giribet G, Sorensen MV, Funch P, Kristensen RM, Sterrer W (2004) Investigations into the phylogenetic position of Micrognathozoa using four molecular loci. Cladistics 20:1–13.

    Article  Google Scholar 

  18. 18.

    Golvan YJ (1969) Systématique des Acanthocéphales (Acanthocephala Rudolphi, 1801). Première Partie. L’Ordre des Palaeacanthocephala Meyer, 1931. Premier fascicule. La Superfamille des Echinorhynchoidae (Cobbold, 1876) Golvan et Houin, 1963. Mém Muséum Natl Hist Nat 57:1–373

    Google Scholar 

  19. 19.

    Gregori M, Aznar FJ, Abollo E, Roura A, González AF, Pascual S (2013) Nyctiphanes couchii as intermediate host for Rhadinorhynchus sp. (Acanthocephala, Echinorhynchidae) from NW Iberian Peninsula waters. Dis Aquat Org 105:9–20.

    CAS  Article  PubMed  Google Scholar 

  20. 20.

    Ha NV, Amin OM, Ngo HD, Heckmann RA (2018) Descriptions of acanthocephalans, Cathayacanthus spinitruncatus (Rhadinorhynchidae) male and Pararhadinorhynchus magnus n. sp. (Diplosentidae), from marine fish of Vietnam, with notes on Heterosentis holospinus (Arhythmacanthidae). Parasite 25:35.

    Article  PubMed  PubMed Central  Google Scholar 

  21. 21.

    Herlyn H, Piskurek O, Schmitz J, Ehlers U, Zischlera H (2003) The syndermatan phylogeny and the evolution of acanthocephalan endoparasitism as inferred from 18S rDNA sequences. Mol Phylogenet Evol 26:155–164.

    CAS  Article  PubMed  Google Scholar 

  22. 22.

    Johnston TH, Edmonds SJ (1947) Australian Acanthocephala No. 5. Trans R Soc Aust 71:13–19

    Google Scholar 

  23. 23.

    Kráľová-Hromadová I, Tietz DF, Shinn AP, Špakulová V (2003) ITS rDNA sequences of Pomphorhynchus laevis (Zoega in Müller, 1776) and P. lucyi William & Rogers, 1984 (Acanthocephala: Palaeacanthocephala). Syst Parasitol 56:141–145.

    Article  Google Scholar 

  24. 24.

    Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874.

    CAS  Article  Google Scholar 

  25. 25.

    Li L, Chen HX, Amin OM, Yang Y (2017) Morphological variability and molecular characterization of Pomphorhynchus zhoushanensis sp. nov. (Acanthocephala: Pomphorhynchidae), with comments on the systematic status of Pomphorhynchus Monticelli, 1905. Parasitol Int 66:693–698.

    CAS  Article  PubMed  Google Scholar 

  26. 26.

    Li L, Chen HX, Yang Y (2018) Morphological and molecular study of Neorhadinorhynchus nudus (Harada, 1938) (Acanthocephala: Cavisomidae) from Auxis thazard Lacepede (Perciformes: Scombridae) in the South China Sea. Acta Parasitol 63:479–485.

    CAS  Article  PubMed  Google Scholar 

  27. 27.

    Lisitsyna OI, Kudlai O, Spraker TR, Tkach VV, Smales LR, Kuzmina TA (2019) Morphological and molecular evidence for synonymy of Corynosoma obtuscens Lincicome, 1943 with Corynosoma austral Johnston, 1937 (Acanthocephala: Polymorphidae). Syst Parasitol 96:105–110.

    Article  Google Scholar 

  28. 28.

    Lisitsyna OI, Kudlai O, Cribb TH, Smit NJ (2019) Three new species of acanthocephalans (Palaeacanthocephala) from marine fishes collected off the East Coast of South Africa. Folia Parasitol 66:012.

    CAS  Article  Google Scholar 

  29. 29.

    Littlewood DTJ, Curini-Galletti M, Herniou EA (2000) The interrelationships of Proseriata (Platyhelminthes: seriata) flatworms tested with molecules and morphology. Mol Phylogenet Evol 16:449–466.

    CAS  Article  PubMed  Google Scholar 

  30. 30.

    Littlewood DTJ, Olson PD (2001) Small subunit rDNA and the phylum platyhelminthes: signal, noise, conflict and compromise. In: Littlewood DTJ, Bray RA (eds) Interrelationships of the platyhelminthes. Taylor and Francis, London, pp 262–278

    Google Scholar 

  31. 31.

    Martínez-Aquino A, Reyna-Fabián ME, Rosas-Valdez R, Razo-Mendivil U, de León GP, García-Varela M (2009) Detecting a complex of cryptic species within Neoechinorhynchus golvani (Acanthocephala: Neoechinorhynchidae) inferred from ITSs and LSU rDNA gene sequences. J Parasitol 95:1040–1047.

    CAS  Article  PubMed  Google Scholar 

  32. 32.

    Milne I, Lindner D, Bayer M, Husmeier D, McGuire G, Marshall DF, Wright F (2009) TOPALiv2: a rich graphical interface for evolutionary analyses of multiple alignments on HPC clusters and multi-core desktops. Bioinformatics 25:126–127.

    CAS  Article  PubMed  Google Scholar 

  33. 33.

    Monks S (2001) Phylogeny of the Acanthocephala based on morphological characters. Syst Parasitol 48:81–115.

    CAS  Article  PubMed  Google Scholar 

  34. 34.

    Nakao M (2016) Pseudoacanthocephalus toshimai sp. nov. (Palaeacanthocephala: Echinorhynchidae), a common acanthocephalan of anuran and urodelan amphibians in Hokkaido, Japan, with a finding of its intermediate host. Parasitol Int 65:323–332.

    Article  PubMed  Google Scholar 

  35. 35.

    Near JT, Garey JR, Nadler SA (1998) Phylogenetic relationships of the acanthocephala inferred from 18S ribosomal DNA sequences. Mol Phylogenet Evol 10:287–298.

    CAS  Article  PubMed  Google Scholar 

  36. 36.

    Perrot-Minnot MJ (2004) Larval morphology, genetic divergence, and contrasting levels of host manipulation between forms of Pomphorhynchus laevis (Acanthocephala). Int J Parasitol 34:45–54.

    Article  PubMed  Google Scholar 

  37. 37.

    Pichelin S, Cribb TH (2001) The status of the Diplosentidae (Acanthocephala: Palaeacanthocephala) and a new family of acanthocephalans from Australian wrasses (Pisces: Labridae). Folia Parasitol 48:289–303.

    CAS  Article  PubMed  Google Scholar 

  38. 38.

    Posada D (2008) jModelTest: phylogenetic model averaging. Mol Biol Evol 25:1253–1256.

    CAS  Article  PubMed  Google Scholar 

  39. 39.

    Smales LR, Adlard RD, Elliot A, Kelly E, Lymbery AJ, Miller TL, Shamsi S (2018) A review of the Acanthocephala parasitising freshwater fishes in Australia. Parasitology 145:249–259.

    CAS  Article  PubMed  Google Scholar 

  40. 40.

    Sobecka E, Szostakowska B, MacKenzie K, Hemmingsen W, Prajsnar S, Eydal M (2012) Genetic and morphological variation in Echinorhynchus gadi Zoega in Müller, 1776 (Acanthocephala: Echinorhynchidae) from Atlantic cod Gadus morhua L. J Helminthol 86:16–25.

    CAS  Article  PubMed  Google Scholar 

  41. 41.

    Steinauer ML, Garcia-Vedrenne AE, Weinstein SB, Kuris AM (2019) Acanthocephalan parasites of the oarfish, Regale cusrusselii (Regalecidae), with a description of a new species of Gymnorhadinorhynchus (Acanthocephala: Gymnorhadinorhynchidae). J Parasitol 105:124–132.

    Article  PubMed  Google Scholar 

  42. 42.

    Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  43. 43.

    Tkach VV, Lisitsyna OI, Crossley JL, Binh TT, Bush SE (2013) Morphological and molecular differentiation of two new species of Pseudoacanthocephalus Petrochenko, 1958 (Acanthocephala: Echinorhynchidae) from amphibians and reptiles in the Philippines, with identification key for the genus. Syst Parasitol 85:11–26.

    Article  PubMed  Google Scholar 

  44. 44.

    Vardić Smrzlić I, Valić D, Kapetanović D, Filipović Marijić V, Gjurčević E, Teskeredžić E (2015) Pomphorhynchus laevis (Acanthocephala) from the Sava River basin: new insights into strain formation, mtDNA-like sequences and dynamics of infection. Parasitol Int 64:243–250.

    CAS  Article  PubMed  Google Scholar 

  45. 45.

    Verweyen L, Klimpel S, Palm HW (2011) Molecular phylogeny of the Acanthocephala (Class Palaeacanthocephala) with a paraphyletic assemblage of the orders Polymorphida, and Echinorhynchida. PLoS ONE 6:e28285.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  46. 46.

    Waindok P, Lehnert K, Siebert U, Pawliczka I, Strube C (2018) Prevalence and molecular characterisation of Acanthocephala in pinnipedia of the North and Baltic Seas. Int J Parasitol Parasites Wildl 7:34–43.

    Article  PubMed  PubMed Central  Google Scholar 

  47. 47.

    Wang Y, Wang P, Wu D (1993) On some Echinorhynchoidea parasites from marine fishes of Fujian province, China. Wuyi Sci J 10:29–39

    Google Scholar 

  48. 48.

    Weaver HJ, Smales LR (2014) Two species of Acanthocephala (Rhadinorhynchidae and Transvenidae) from elasmobranchs from Australia. Comp Parasitol 81:110–113.

    Article  Google Scholar 

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We would like to acknowledge the laboratory facilities provided by Department of Zoology, Chaudhary Charan Singh University, Meerut, India. This work was supported by institutional Grants from the Department of Biology, Brigham Young University, Provo, Utah and the Institute of Parasitic Diseases, Scottsdale, Arizona, USA.

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Correspondence to Omar M. Amin.

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Chaudhary, A., Amin, O.M., Heckmann, R. et al. The Molecular Phylogeny of Pararhadinorhynchus magnus Ha, Amin, Ngo, Heckmann, 2018 (Acanthocephala: Rhadinorhynchidae) from Scatophagus argus (Linn.) (Scatophagidae) in Vietnam. Acta Parasit. 65, 610–619 (2020).

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  • Acanthocephala
  • Molecular profile
  • Pararhadinorhynchus magnus
  • Vietnam