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Serodiagnosis of Fasciola gigantica Infection in Buffaloes with Native Cathepsin-L Proteases and Recombinant Cathepsin L1-D

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Abstract

Aim

Serodiagnosis of Fasciola gigantica natural infection in buffaloes with recombinant cathepsin L1-D and native cathepsin-L protease antigens.

Methods

The recombinant cat L1-D antigen was expressed in prokaryotic expression system and native cathepsin-L proteases were purified by alcoholic fractionation from adult F. gigantica flukes. Buffaloes (n  = 325) were screened for anti-Fasciola antibodies with the above antigens in immunoglobulin-G-enzyme linked immunosorbent assay (IgG-ELISA).

Results

The recombinant cat L1-D antigen showed positive reactivity with 101/122 necropsy positive animals but 21/122 necropsy confirmed positive animals were negative in this ELISA (sensitivity 82.8%). However, 30/203 (14.8%) necropsy negative animals for Fasciola were seropositive with specificity of 85.2%. With native cat-L protease, 104/122 necropsy confirmed positive animals were ELISA positive but 18/122 necropsy positive animals were seronegative, thereby depicting the sensitivity of 85.2%. But ELISA with this antigen showed 27/203 (13.3%) necropsy negative animals as positive (specificity 86.7%).

Conclusions

Comparative evaluation of both the antigens showed that they are suitable for serodiagnosis of F. gigantica infection in buffalo herds.

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Acknowledgements

The first and second authors are thankful to the Head, Department of Biotechnology, SHUATS, Prayagraj, U.P. for providing facilities for this research work. The authors are also thankful to the Director, CARI-Indian Veterinary Research Institute, Izatnagar for allowing the first author to do the part of this research work at his institute as a trainee. We are also thankful to M/S Mary Nisha for her assistance in the preparation of the manuscript.

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Authors and Affiliations

  1. Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, U.P., 211007, India

    Andleeb Aftab & Rohit Lall

  2. ICAR-Indian Veterinary Research Institute, Izatnagar, U.P., 243122, India

    Savita Bisen, Arun Anandanarayanan, Ajayta Rialch, Jayanta K. Chamuah, Shobha Yadav, M. Silamparasan & O. K. Raina

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  1. Andleeb Aftab
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Aftab, A., Lall, R., Bisen, S. et al. Serodiagnosis of Fasciola gigantica Infection in Buffaloes with Native Cathepsin-L Proteases and Recombinant Cathepsin L1-D. Acta Parasit. 65, 413–421 (2020). https://doi.org/10.2478/s11686-020-00177-3

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