Dactylogyrus spp. (Monogenea, Dactylogyridae) from the Gills of Enteromius spp. (Cypriniformes, Cyprinidae) from the Limpopo Province, South Africa with Descriptions of Three New Species



Monogenean parasites of the genus Dactylogyrus Diesing, 1850 parasitize mostly gills of cyprinoids hosts. Of 100 species currently known from African continent, approximately 35 have been described from Enteromius spp. Results of recent studies indicate that there are still many undescribed species of the genus Dactylogyrus in South Africa and systematic surveys can bring many new findings.


During the period April 2015–May 2016, three species of the genus Enteromius were sampled from eight localities across Limpopo Province. Monogenean parasites were collected from the gills of the hosts using stereomicroscopes. Morphometric analysis of the hard parts of the attachment organ and male copulatory organs were performed to confirm species identity.


Presence of three new and three previously described Dactylogyrus species is reported. Newly described species include: Dactylogyrus afrohamiltoni sp. nov. from Enteromius afrohamiltoni; Dactylogyrus limpopoensis sp. nov. and Dactylogyrus letabaensis sp. nov. from Enteromius unitaeniatus. In addition, Dactylogyrus afrolongicornis, Dactylogyrus allolongionchus and Dactylogyrus myersi were identified from Enteromius trimaculatus. Newly identified species possess morphometric characters based on which they can be clearly identified from currently known species.


Present results show that small barbs, especially those not previously studied for monogenean parasites, are potentially very interesting target to study to recover new species of the genus Dactylogyrus and to bring new contribution to the knowledge of the diversity of African parasites.

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  1. 1.

    Christison K (2002) Branchial monogenean parasites (Monogenea: Dactylogyridae) of fishes from the Okavango River and Delta, Botswana. PhD Thesis, University of the Free State, South Africa, pp 155

  2. 2.

    Crafford D, Luus-Powell W, Avenant-Oldewage A (2012) Monogenean parasite species descriptions from Labeo spp. hosts in the Vaal Dam, South Africa. Afr Zool 47:216–228. https://doi.org/10.3377/004.047.0206

    Article  Google Scholar 

  3. 3.

    Crafford D, Luus-Powell W, Avenant-Oldewage A (2014) Monogenean parasites from fishes of the Vaal Dam, Gauteng Province, South Africa. I. winter survey versus summer survey comparison from Labeo capensis (Smith, 1841) and Labeo umbratus (Smith, 1841) hosts. Acta Parasitol 59:17–24. https://doi.org/10.2478/s11686-014-0205-7

    Article  PubMed  Google Scholar 

  4. 4.

    Ergens R (1969) The suitability of ammonium picrate-glycerine in preparing slides of lower Monogenoidea. Folia Parasitol 16:320

    Google Scholar 

  5. 5.

    Gibson DI, Timofeeva TA, Gerasev PI (1996) A catalogue of the nominal species of the monogenean genus Dactylogyrus Diesing, 1850 and their host genera. Syst Parasitol 35:3–48. https://doi.org/10.1007/BF00012180

    Article  Google Scholar 

  6. 6.

    Guégan J-F, Lambert A (1991) Dactylogyrids (Platyhelminthes: Monogenea) of Labeo (Teleostei: Cyprinidae) from West African coastal rivers. J Helminthol Soc Wash 58:85–99

    Google Scholar 

  7. 7.

    Gussev AV (1962) Order dactylogyridea. In: Bychovskaya-Pavlovskaya E et al (eds) Key to parasites of freshwater fish of the USSR. House of Academy of Sciences of the USSR, Moscow, p 919 (in Russian; English translation IPST, Ser. No. 1136, Jerusalem, 1964)

    Google Scholar 

  8. 8.

    Jarkovský J, Morand S, Šimková A, Gelnar M (2004) Reproductive barriers between congeneric monogenean parasites (Dactylogyrus: Monogenea): attachment apparatus morphology or copulatory organ incompatibility? Parasitol Res 92:95–105. https://doi.org/10.1007/s00436-003-0993-4

    Article  PubMed  Google Scholar 

  9. 9.

    Malmberg G (1957) On the occurrence of Gyrodactylus on Swedish fishes. Skrifterutgivna av Sodra Sveriges Fiskeriforening, (1956): 19–76. (In Swedish, with description of species and a summary in English)

  10. 10.

    Mashego S (1983) South African monogenetic parasites of the genus Dactylogyrus: new species and records (Dactylogyridae: Monogenea). Ann Transvaal Mus 33:337–346

    Google Scholar 

  11. 11.

    Mashego SN, Matlou KS (2018) A new Dactylogyrus species (Dactylogyridae: Monogenea) from Enteromius mattozi, Cyprinidae, at Piet Gouws Dam, South Africa. Afr Zool 53:107–111. https://doi.org/10.1080/15627020.2018.1521301

    Article  Google Scholar 

  12. 12.

    Matla MM (2012) Helminth ichthyo-parasitic fauna of a South African sub-tropical lake. PhD Thesis. University of Limpopo, South Africa, p 281

  13. 13.

    Mbokane EM (2011) Metazoan parasites and health of selected cyprinids at Nwanedi-Luphephe dams. MSc Dissertation, University of Limpopo, South Africa, p 160

  14. 14.

    Mbokane EM, Matla MM, Theron J, Luus-Powell WJ (2015) Seasonal dynamics and occurrences of three Dactylogyrus species on the gills of three cyprinids at Nwanedi-Luphephe dams in Limpopo province, South Africa. Afr Zool 50:119–125. https://doi.org/10.1080/15627020.2015.1021175

    Article  Google Scholar 

  15. 15.

    Mizelle JD (1936) New species of trematodes from gills of Illinois fishes. Am Midl Nat 17:785–806

    Article  Google Scholar 

  16. 16.

    Musilová N, Řehulková E, Gelnar M (2009) Dactylogyrids (Platyhelminthes: Monogenea) from the gills of the African carp, Labeo coubie Rüppell (Cyprinidae), from Senegal, with descriptions of three new species of Dactylogyrus and the redescription of Dactylogyrus cyclocirrus Paperna, 1973. Zootaxa 2241:47–68

    Article  Google Scholar 

  17. 17.

    Olivier P, Luus-Powell WJ, Saayman J (2009) Report on some monogenean and clinostomid infestations of freshwater fish and waterbird hosts in Middle Letaba Dam, Limpopo Province, South Africa. Onderstepoort J Vet Res 76:187–199

    CAS  Article  Google Scholar 

  18. 18.

    Paperna I (1973) New species of Monogenea (Vermes) from African freshwater fish: a preliminary report. Rev Zool Bot Afr 87:505–518

    Google Scholar 

  19. 19.

    Paperna I (1979) Monogenea of inland water fish in Africa. Ann Musee Roy Afr Centr Sci Zool 8(226):1–131

    Google Scholar 

  20. 20.

    Price CE, McClellan S, Druckenmiller A, Jacobs LG (1969) The monogenean parasites of African fishes. X. Two additional Dactylogyrus species from South African Barbus hosts. Proc Biol Soc Wash 82:461–468

    Google Scholar 

  21. 21.

    Řehulková E, Seifertová M, Přikrylová I, Francová K (2018) Monogenea. In: Scholz T, Vanhove MPM, Smit N, Jayasundera Z, Gelnar M (eds) A guide to the parasites of African freshwater fishes. RBINS´Scinetific Publication Unit, Abc Taxa, Brussels, pp 185–243

    Google Scholar 

  22. 22.

    Skelton PH (2001) A complete guide to the freshwater fishes of southern Africa. Struik Publishers, Cape Town, p 395

    Google Scholar 

  23. 23.

    Swanepoel PJ (2015) Parasites of Barbus species (Cyprinidae) of southern Africa. MSc Dissertation. University of the Free State, South Africa, p 190

  24. 24.

    Šimková A, Desdevises Y, Gelnar M, Morand S (2000) Co-existence of nine gill ectoparasites (Dactylogyrus: Monogenea) parasitising the roach (Rutilus L.): history and present ecology. Int J Parasitol 30:1077–1088

    Article  Google Scholar 

  25. 25.

    Šimková A, Ondračková M, Gelnar M, Morand S (2002) Morphology and coexistence of congeneric ectoparasite species: reinforcement of reproductive isolation? Biol J Linn Soc 76:125–135. https://doi.org/10.1046/j.1095-8312.2002.00056x

    Article  Google Scholar 

  26. 26.

    Šimková A, Plaisance L, Matějusová I, Morand S, Verneau O (2003) Phylogenetic relationships of the Dactylogyridae Bychowsky, 1933 (Monogenea: Dactylogyridea): the need for the systematic revision of the Ancyrocephalinae Bychowsky, 1937. Syst Parasitol 54:1–11. https://doi.org/10.1023/A:1022133608662

    Article  PubMed  Google Scholar 

  27. 27.

    Šimková A, Benovics M, Rahmouni I, Vukić J (2017) Host-specific Dactylogyrus parasites revealing new insights on the historical biogeography of Northwest African and Iberian cyprinid fish. Parasite Vectors 10:589. https://doi.org/10.1186/s13071-017-2521-x

    Article  Google Scholar 

  28. 28.

    Truter M, Přikrylová I, Malherbe W, Smit NJ (2016) First report of metazoan parasites from the cichlid Pseudocrenilabrus philander and the cyprinid Enteromius paludinosus in a South African Ramsar wetland. Afr J Aquat Sci 41:499–504. https://doi.org/10.2989/16085914.2016.1246357

    Article  Google Scholar 

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The authors sincerely thank Prof. A. Jooste, Prof. A. Addo-Bediako, Prof. W. J. Luus-Powell, Dr. K. Bal, Ms. B. M. Kekana, Mr. H. E. Hattingh, Dr. J. R. Sara, Mr. G. Geldenhys, Mr. A. Hlungwani and Mr. S. T. Matjee for assistance during field work excursions. The National Research Foundation (NRF) Innovation Scholarship and the Vlaamse Interuniversitaire Raad-University Development Corporation (VLIR-UOS) is acknowledged for financial support, and the Department of Biodiversity, University of Limpopo (UL) for availability of research facilities. This is contribution number XX from the NWU-Water Research Group. Any opinion, finding and conclusion or recommendation expressed in this material is that of the author(s) and the NRF does not accept any liability in this regard.

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Raphahlelo, M.E., Přikrylová, I. & Matla, M.M. Dactylogyrus spp. (Monogenea, Dactylogyridae) from the Gills of Enteromius spp. (Cypriniformes, Cyprinidae) from the Limpopo Province, South Africa with Descriptions of Three New Species. Acta Parasit. 65, 396–412 (2020). https://doi.org/10.2478/s11686-020-00175-5

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  • Monogeneans
  • Dactylogyrus
  • Cyprinidae
  • Ectoparasites