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Acta Parasitologica

, Volume 64, Issue 3, pp 679–685 | Cite as

Isolated Human and Livestock Echinococcus granulosus Genotypes Using Real-Time PCR of cox1 Gene in Northeast Iran

  • Mohammad-Ali Mohaghegh
  • Hossein Yousofi-Darani
  • Amir Hossein Jafarian
  • Seyed-Reza Mirbadie
  • Majid Fasihi-Harandi
  • Reza Ghavimi
  • Zahra Jabalameli
  • Mehdi Azami
  • Mohsen Mohammadi
  • Seyed Hossein HejaziEmail author
Original Paper

Abstract

Purpose

Cystic echinococcosis (CE), caused by the metacestode of Echinococcus granulosus, is highly endemic over large parts of Iran. The purpose of this study was to investigate the prevalence rate of hydatidosis and mitochondrial cox1 real-time PCR with high-resolution melting curve (HRM) analysis of E. granulosus isolated from human and livestock.

Methods

In this cross-sectional study, 61 formalin-fixed paraffin-embedded tissue isolates were collected from human CE cases and 83 hydatid cysts from the liver and lung lesions of the livestock in Khorasan Razavi province, Northeast Iran. DNA was extracted from each isolate and amplified by real-time PCR and analyzed using the HRM method.

Results

The HRM analysis using the cox1 gene of 40 E. granulosus human isolates showed that 35 (87.5%), 4 (10%), and 1 (2.5%) of the isolates were categorized as G1, G3, and G6 genotypes, respectively. Out of the total 1342 livestock inspected, 39 (4%) goats and 44(12%) cattle were found harboring hydatid cysts all belonging to E. granulosus sensu stricto.

Conclusion

The results confirmed that the high prevalence of E. granulosus sensu stricto in intermediate hosts is remarkable in northeast of Iran coupled with the high prevalence of infection in livestock, which reinforced the need for hydatidosis control programs in this region.

Keywords

Echinococcus granulosus G1 genotype HRM Livestock Human 

Notes

Acknowledgements

The authors would like to greatly appreciate the personnel of the Parasites Research Laboratory, Isfahan University of Medical Sciences. This work represents the PhD thesis of first author.

Funding

This investigation was supported by a Grant from Isfahan University of Medical Sciences (Grant 394825).

Compliance with ethical standards

Conflict of interest

The author(s) declare that they have no competing interests.

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Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2019

Authors and Affiliations

  • Mohammad-Ali Mohaghegh
    • 1
  • Hossein Yousofi-Darani
    • 1
  • Amir Hossein Jafarian
    • 2
  • Seyed-Reza Mirbadie
    • 3
  • Majid Fasihi-Harandi
    • 4
  • Reza Ghavimi
    • 5
  • Zahra Jabalameli
    • 6
  • Mehdi Azami
    • 7
  • Mohsen Mohammadi
    • 8
  • Seyed Hossein Hejazi
    • 9
    Email author
  1. 1.Department of Parasitology and Mycology, School of MedicineIsfahan University of Medical SciencesIsfahanIran
  2. 2.Department of Pathology, Faculty of MedicineMashhad University of Medical SciencesMashhadIran
  3. 3.School of MedicineShahroud University of Medical SciencesShahroudIran
  4. 4.School of Medicine, Research Center for Hydatid Disease in IranKerman University of Medical SciencesKermanIran
  5. 5.Department of Pharmaceutical Biotechnology, School of PharmacyIsfahan University of Medical SciencesIsfahanIran
  6. 6.Department of Laboratory Sciences, School of Paramedical SciencesTorbat Heydariyeh University of Medical SciencesTorbat HeydariyehIran
  7. 7.Skin Diseases and Leishmaniasis Research CenterIsfahan University of Medical SciencesIsfahanIran
  8. 8.The Persian Gulf Marine Biotechnology Research Center, Persian Gulf Biomedical Sciences Research InstituteBushehr University of Medical SciencesBushehrIran
  9. 9.Department of Parasitology and Mycology, School of Medicine, Skin Diseases and Leishmaniasis Research CenterIsfahan University of Medical SciencesIsfahanIran

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