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Acta Parasitologica

, Volume 64, Issue 4, pp 797–806 | Cite as

Redescription and First Nucleotide Sequences of Rhinobatonchocotyle pacifica Oliva & Luque, 1995 (Monogenea: Hexabothriidae), a Parasite of Pseudobatos planiceps (Garman, 1880) (Rhinopristiformes: Rhinobatidae) from Peru

  • Jhon D. Chero
  • Celso L. Cruces
  • Gloria Sáez
  • Ana C. A. Camargo
  • Cláudia Portes Santos
  • José L. LuqueEmail author
Original Paper
  • 28 Downloads

Abstract

Introduction

Specimens of Rhinobatonchocotyle pacifica Oliva & Luque, 1995 (Hexabothriidae) were collected from the gill filaments of Pacific guitarfish, Pseudobatos planiceps (Garman, 1880) (Rhinopristiformes: Rhinobatidae), from off the coast of Chorrillos, Lima, Peru. Since the original description of R. pacifica was incomplete, we herein redescribe R. pacifica based on re-examination of the holotype, paratypes, and newly collected voucher specimens. Nucleotide sequences (18S, 28S) for R. pacifica are presented for the first time, and a phylogenetic analysis conducted.

Materials and Methods

Monogeneans were examined with light microscopy and partial sequences of 18S and 28S rDNA genes were obtained.

Results

The following taxonomically important morphological features are described: presence of seminal receptacle, spiniform papillae within oral cavity, haptoral sucker sclerites and anchors, hook surface striations, muscular pad associated with each haptoral sucker sclerite (except sclerite 1), muscular bulbs in appendix suckers, and oötype lacking longitudinal rows of large cells (oötype not côtelé). The genital complex is detailed. The diagnosis of Rhinobatonchocotyle is emended to include species with or without seminal receptacle. Representative nucleotide sequences of partial 18S and 28S rDNA genes of R. pacifica were obtained for the first time and used for reconstructing the phylogeny of Hexabothriidae.

Conclusion

The phylogenetic reconstruction confirmed the species within the Hexabothriidae.

Keywords

Chondrichthyans Ectoparasites Monogeneans Pacific Ocean Phylogeny Taxonomy 

Notes

Acknowledgements

To Anna Phillips (USNM) for allowing us access to specimens under her care. We also thank Griselda Pulido-Flores for providing us with valuable literature on hexabothriids. The authors are grateful to the following people who helped to the collection of fishes in Peru: Ivette Cuellar, Eva G. Huancachoque and Cynthia E. Rodríguez, all from the National University Federico Villarreal (UNFV). José L. Luque and Cláudia P. Santos were supported by a Researcher fellowship from the Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil (CNPq). Jhon D. Chero and Celso L. Cruces were supported by a student fellowship from the Coordenação de Aperfeiçoamento de Pessoal do Ensino Superior, Brazil (CAPES)—Finance Code 001.

References

  1. 1.
    Miloslavich P, Klein E, Díaz JM, Hernández CE, Bigatti G, Campos L, Artigas F, Castillo J, Penchaszadeh PE, Neill PE, Carranza A, Retana MV, de Astarloa JMD, Lewis M, Yorio P, Piriz ML, Rodríguez D, Yoneshigue-Valentin Y, Gamboa L, Martin A (2011) Marine biodiversity in the Atlantic and Pacific coasts of South America: knowledge and gaps. PLoS ONE 6:e14631.  https://doi.org/10.1371/journal.pone.0014631 CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Cornejo R, Vélez-Zuazo X, González-Pestana A, Kouri JC, Mucientes C (2015) An updated checklist of Chondrichthyes from the southeast Pacific off Peru. Check List 11:1–7.  https://doi.org/10.15560/11.6.1809 CrossRefGoogle Scholar
  3. 3.
    Eiras JC, Velloso AL, Pereira J Jr (2016) Parasitos de Peixes Marinhos da América do Sul. Editora FURG, Rio Grande, p 441Google Scholar
  4. 4.
    Luque JL, Cruces C, Chero J, Paschoal F, Alves PV, Da Silva AC, Sánchez L, Iannacone J (2016) Checklist of metazoan parasites of fishes from Perú. Neotrop Helminthol 10:301–375Google Scholar
  5. 5.
    Chero JD, Cruces CL, Sáez G, Camargo ACA, Santos CP, Luque JL (2018) Hypanocotyle bullardi n. gen. n. sp. (Monogenea: Hexabothriidae) from gill of the diamond stingray Hypanus dipterurus (Jordan et Gilbert) (Myliobatiformes: Dasyatidae) in the Southeastern Pacific Ocean off Peru. Parasitol Int 67:425–430.  https://doi.org/10.1016/j.parint.2018.03.010 CrossRefPubMedGoogle Scholar
  6. 6.
    Chero JD, Cruces CL, Sáez G, Luque JL (2018) A new genus and species of the Dasybatotreminae Bychowsky, 1957 (Monogenea: Monocotylidae), parasitic on Hypanus dipterurus (Jordan & Gilbert) (Myliobatiformes: Dasyatidae) in the Southeastern Pacific Ocean off Peru. Zootaxa 1:347–356.  https://doi.org/10.11646/zootaxa.4527.3.4 CrossRefGoogle Scholar
  7. 7.
    Martínez R, Tantaleán M, Mondragón-Martínez A, Pulido E, Delgado A (2016) Nuevos reportes de monogeneos en peces marinos de la costa peruana. Peruv J Parasitol 24:8–14Google Scholar
  8. 8.
    Eschmeyer WN, Fricke R, Van der Laan R (eds) (2018) Catalog of fishes: genera, species, references. Available at www.calacademy.org, version 03/2018. Accessed March 2018
  9. 9.
    Humason GL (1979) Animal tissue techniques, 4th edn. W. H. Freeman and Company, San Francisco, p 661Google Scholar
  10. 10.
    Billings AN, Teltow GJ, Weaver SC, Walker DH (1998) Molecular characterization of a novel Rickettsia species from Ixodes scapularis in Texas. Emerg Infect Dis J 4:305–309.  https://doi.org/10.3201/eid0402.980221 CrossRefGoogle Scholar
  11. 11.
    Hassouna N, Michot B, Bachellerie JP (1984) The complete nucleotide sequence of mouse 28 s rRNA gene. Implications for the eukaryotes. Nucleic Acids Res 12:3563–3583.  https://doi.org/10.1093/nar/12.8.3563 CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Sinnappah ND, Lim LHS, Rohde K, Tinsley R, Combes C, Verneau O (2001) A paedomorphic parasite associated with a neotenic amphibian host: phylogenetic evidence suggests a revised systematic position for Sphyranuridae within anuran and turtle polystomatoineans. Mol Phylogenet Evol 18:189–201.  https://doi.org/10.1006/mpev.2000.0877 CrossRefPubMedGoogle Scholar
  13. 13.
    Kumar S, Stecher G, Tamura K (2015) MEGA7: molecular evolutionary genetics analysis version 7.0. Molecular biology and evolution (submitted). (Publication PDF at http://www.kumarlab.net/publications)
  14. 14.
    Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410.  https://doi.org/10.1016/S0022-2836(05)80360-2 CrossRefPubMedGoogle Scholar
  15. 15.
    Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680.  https://doi.org/10.1093/nar/22.22.4673 CrossRefPubMedPubMedCentralGoogle Scholar
  16. 16.
    Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376.  https://doi.org/10.1007/BF01734359 CrossRefPubMedGoogle Scholar
  17. 17.
    Boeger WA, Kritsky DC (1989) Phylogeny, coevolution, and revision of the Hexabothriidae Price, 1942 (Monogenea). Int J Parasitol 19:425–440.  https://doi.org/10.1016/0020-7519(89)90099-4 CrossRefGoogle Scholar
  18. 18.
    Doran DJ (1953) New monogenetic trematodes from the shovelnose guitar fish, Rhinobatos productus (Ayres). J Parasitol 39:145–151.  https://doi.org/10.2307/3274109 CrossRefPubMedGoogle Scholar
  19. 19.
    Oliva ME, Luque JL (1995) Rhinobatonchocotyle pacifica n. sp. (Cercomeromorphae: Monogenea: Hexabothriidae) parasite of Rhinobatos planiceps (Rhinobatidae) from Northern Chile. Revista de Biología Marina 30:1–5Google Scholar
  20. 20.
    Pollerspöck J, Straube N (2015) Bibliography database of living/fossil sharks, rays and chimaeras (Chondrichthyes: Elasmobranchii, Holocephali): host-parasite list and parasite-host list. World Wide Web electronic publication. www.shark-references.com, version (04/2015).  https://doi.org/10.13140/RG.2.1.3636.6887
  21. 21.
    Bullard SA, Dippenaar SM (2003) Branchotenthes robinoverstreeti n. gen. and n. sp. (Monogenea: Hexabothiidae) from gill filaments of the bowmouth guitarfish, Rhina ancylostoma (Rhynchobatidae), in the Indian Ocean. J. Parasitol 89:595–601.  https://doi.org/10.1645/0022-3395(2003)089%5b0595:BRNGAN%5d2.0.CO;2 CrossRefPubMedGoogle Scholar
  22. 22.
    Patella R, Bullard SA (2013) Hexabothriids of devil rays (Mobulidae): new genus and species from gill of Mobula hypostoma in the Northern Gulf of Mexico and redescription of a congener from Mobula rochebrunei in the Eastern Atlantic Ocean. J Parasitol 99:856–867.  https://doi.org/10.1645/12-153.1 CrossRefPubMedGoogle Scholar
  23. 23.
    Watson DE, Thorson TB (1976) Helminths from elasmobranchs in central American fresh waters. In: Thorson TB (ed) Investigations of the Ichthyofauna of Nicaraguan Lakes. School of Life Sciences, University of Nebraska-Lincoln, Lincoln, pp 629–640Google Scholar
  24. 24.
    Ogawa K (1991) Ectoparasites of sawfish, Pristis microdon, caught in freshwaters of Australia and Papua New Guinea. In: Shimizu M, Taniuchi T (eds) Studies on Elasmobranchs collected from seven river systems in Northern Australia and Papua New Guinea, vol 3. Nature culture. University of Tokyo, Tokyo, pp 91–102Google Scholar
  25. 25.
    Quiterio-Rendon G, Monks S, Pulido-Flores G (2018) Neonchocotyle violantei n. sp. (Monogenea, Hexabothriidae) from Pseudobatos lentiginosus (Rhinopristiformes, Rhinobatidae) of Yucatán, Gulf of Mexico. Braz J Vet Parasitol 27:33–41.  https://doi.org/10.1590/S1984-29612017077 CrossRefGoogle Scholar
  26. 26.
    Tantaleán M, Morales E, Hermes E (1998) Una nueva especie y nuevo registro de monogeneos parásitos en peces marinos del Perú. Parasitología al día 22:41–44.  https://doi.org/10.4067/S0716-07201998000100008 CrossRefGoogle Scholar

Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2019

Authors and Affiliations

  1. 1.Programa de Pós-Graduação em Biologia Animal da Universidade Federal Rural do Rio de JaneiroSeropédicaBrazil
  2. 2.Programa de Pós-Graduação em Ciências Veterinárias da Universidade Federal Rural do Rio de JaneiroSeropédicaBrazil
  3. 3.Laboratorio de Parasitología General y Especializada, Facultad de Ciencias Naturales y MatemáticaUniversidad Nacional Federico Villarreal (UNFV)LimaPeru
  4. 4.Laboratório de Avaliação e Promoção da Saúde Ambiental, Instituto Oswaldo CruzFundação Oswaldo CruzRio de JaneiroBrazil
  5. 5.Departamento de Parasitologia AnimalUniversidade Federal Rural do Rio de JaneiroSeropédicaBrazil

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