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First Report on the Genus Gyrinicola Yamaguti, 1938 from India with a Description of a New Species

  • Pallab Maity
  • Anjum N. RizviEmail author
  • Charles R. Bursey
  • Kailash Chandra
Original Paper

Abstract

Introduction

Gyrinicola dehradunensis sp. nov. from the intestine of tadpoles of the small paa frog, Nanorana minica, from Dehradun (Uttarakhand), India is described and illustrated. Gyrinicola dehradunensis is the first species of the genus recorded from India and the second species recorded from Asia.

Methods

Light microscopy was used for the identification of nematodes using BX53 DIC/BF Olympus research microscope with an attached DP27 digital camera. Drawings for the description of the new species were made from photomicrographs. For molecular studies, DNA was isolated using Qiagen, DNeasy® Blood and Tissue Kit and amplified using r-DNA ITS-18S, and ITS-1.58S primers.

Results

The new species is differentiated from the known species by the absence of larvated eggs and the presence of a tooth-like projection in the buccal region. N. minica is a new host record for the genus. Four r-DNA ITS1 sequences of the new species have been submitted to the NCBI Genbank.

Keywords

Gyrinicola dehradunensis sp. nov. India Tadpole Nanorana minica 

Notes

Acknowledgements

Authors are grateful to the Director, Zoological Survey of India (ZSI), Kolkata for facilities and encouragements. Authors are also thankful to the Officer in Charge, and Field staff of ZSI, Dehradun.

References

  1. 1.
    Araujo P, Artigas PT (1982) Gyrinicola chabaudi n. sp. (Nematoda, Pharyngodonidae) Oxyurideo encontrada em girinos. Memórias Inst Butantan (São Paulo) 44/45:383–390Google Scholar
  2. 2.
    Adamson ML (1981) Development and transmission of Gyrinicola batrachiensis (Walton, 1929) Adamson, 1981 (Pharyngodonidae: Oxyuroidea). Can J Zool 59:1351–1367CrossRefGoogle Scholar
  3. 3.
    Brigitte P, Odile B, Jean-Paul L, Pierre J (2008) Gyrinicola chabadamsoni n. sp. and G. tba (Dinnik 1933) (Nematoda, Oxyuroidea) from tadpoles of the hybridogenetic complex Rana lessonae-esculenta (Amphibia, Ranoidea). Zootaxa 1764:25–39.  https://doi.org/10.5281/zenodo.181991 CrossRefGoogle Scholar
  4. 4.
    Cherry T, Szalanski AL, Todd TC, Powers TO (1997) The internal transcribed spacer region of Belonolaimus (Nemata: Belonolaimidae). J Nematol 29:23–29PubMedPubMedCentralGoogle Scholar
  5. 5.
    Childress JN, Rogers SC, Bolek MG, Langford GJ (2017) Reproductive plasticity in the nematode Gyrinicola batrachiensis: does an intermediate reproductive strategy exist in sexually reproducing, didelphic pinworms? J Parasitol 103:663–668.  https://doi.org/10.1645/17-30 CrossRefPubMedGoogle Scholar
  6. 6.
    Dinnik JA (1930) Data on the fauna of the freshwater parasitic worms in the Caucasus. Raboty Severo-Kavkazskoi gidrobiologicheskoi stantsii pri Gorskom sel’sko-khoziaistvennom Institute 3:87–90 (in Russian)Google Scholar
  7. 7.
    Fabio SP, Pinheiro NL, Sales A (2000) Evidencia histological de hermafroditismo em Gyrinicola chabaudi Araujo and Artigas, 1982 (Nematoda, Pharyngodonidae). Bol Mus Nac Rio J Zool 417:1–6Google Scholar
  8. 8.
    Frost DR (2019) Amphibian species of the world: an online reference. Version 6.0 electronic database accessible at http://research.amnh.org/herpetology/amphibia/index.html. American Museum of Natural History, New York, USA. Accessed 6 June 2019
  9. 9.
    Goldberg SR, Bursey CR, Nieto N, Bettaso J (2004) Rana aurora (Northern red-legged frog). Endoparasites. Herpetol Rev 35:161–162Google Scholar
  10. 10.
    Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
  11. 11.
    Hossack BR, Lowe WH, Honeycutt RK, Parks SA, Corn RS (2013) Interactive effects of wildfire, forest management, and isolation on amphibian and parasite abundance. Ecol Appl 23:479–492CrossRefPubMedGoogle Scholar
  12. 12.
    Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549CrossRefPubMedPubMedCentralGoogle Scholar
  13. 13.
    Ohler A, Dutta S, Shrestha TK (2004) Nanorana minica. The IUCN Red List of Threatened Skpsecies IUCN. 2004: e.T58432A11780511. http://dx.doi.org/110.2305/IUCN.UK.2004.RLTS.T58432/a11780511. Accessed 5 June 2019
  14. 14.
    Pierce CC, Shannon RP, Bolek MG (2018) Distribution and reproductive plasticity of Gyrinicola batrachiensis (Oxyuroidea: Pharyngdonidae) in tadpoles of five anuran species. Parasitol Res 117:461–470.  https://doi.org/10.1007/s00436-017-57234 CrossRefPubMedGoogle Scholar
  15. 15.
    Pryor GS, Greiner EC (2004) Expanded geographical range, new host accounts, and observations of the nematode Gyrinicola batrachiensis (Oxyuroidea: Pharyngodonidae) in tadpoles. J Parasitol 90:189–191CrossRefPubMedGoogle Scholar
  16. 16.
    Pryor GS, Bjorndal KA (2005) Effects of the nematode Gyrinicola batrachiensis on development, gut morphology, and fermentation in bullfrog tadpoles (Rana catesbeiana): a novel mutualism. J Exp Zool 303A:704–712CrossRefGoogle Scholar
  17. 17.
    Rhoden HR, Bolek MG (2011) Distribution and reproductive strategies of Gyrinicola batrachiensis (Oxyuroidea: Pharyngodonidae) in larvae of eight species of amphibians from Nebraska. J Parasitol 97:629–635.  https://doi.org/10.1645/GE-2670.1 CrossRefPubMedGoogle Scholar
  18. 18.
    Rhoden HR, Bolek MG (2012) Helminth and leech community structures in tadpoles and caudatan larvae of two amphibian species from western Nebraska. J Parasitol 98:236–244.  https://doi.org/10.1645/GE-2771.1 CrossRefPubMedGoogle Scholar
  19. 19.
    Rhoden HR, Bolek MG (2015) Helminth community structure in tadpoles of northern leopard frogs (Rana pipiens) and Woodhouse’s toads (Bufo woodhousii) from Nebraska. Parasitol Res 114:4685–4692.  https://doi.org/10.1007/s00436-015-4716-4 CrossRefPubMedGoogle Scholar
  20. 20.
    Rizvi AN, Bursey CR, Bhutia PT (2012) Three new species of Digenea (Batrachotrematidae) in Nanorana minica (Anura, Dicroglossidae) from Uttarakhand, India. Acta Parasitol 57:154–159.  https://doi.org/10.2478/s11686-012-0023-8 CrossRefPubMedGoogle Scholar
  21. 21.
    Rizvi AN, Bursey CR, Bhutia PT (2013) Two new species of Rhabdias (Nematoda: Rhabditida: Rhabdiasidae) in anuran hosts from Dehradun (Uttarakhand), India. J Parasitol 99:303–306.  https://doi.org/10.1645/GE-3179.1 CrossRefPubMedGoogle Scholar
  22. 22.
    Souza FLD Jr, Souza CWOD, Martins ML (1991) Gyrinicola chabaudi Arajuo and Artigas 1982 (Nematoda, Pharyngodonidae) description of male specimens collected from tadpoles. Rev Bras Biol 51:585–588Google Scholar
  23. 23.
    Vojtkova L (1974) Erster fund der nematode Thelandros tba (Dinnik, 1930) Volgar, 1959 in der Tschechoslowakei. Scr Fac Sci Univ Purkyn Brun 4:91–96Google Scholar
  24. 24.
    Vojtkova L (1976) Nematoda (Hlistice) obojzivelniku CSSR I. Dospele hlistice. Folia Fac Sci Univ Purkyn Brun 17:5–80Google Scholar
  25. 25.
    Vrain TC, Wakarchuk DA, Levesque AC, Hamilton RI (1992) Intraspecific rDNA restriction fragment length polymorphism in the Xiphinema americanum group. Fundam Appl Nematol 15:563–573Google Scholar
  26. 26.
    Walton AC (1929) Studies on some nematodes on North American frogs. J Parasitol 15:227–240CrossRefGoogle Scholar
  27. 27.
    Yamaguti S (1938) Studies on the helminth fauna of Japan. Part 23. Two new species of amphibian nematodes. Jpn J Zool 7:603–607Google Scholar

Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2019

Authors and Affiliations

  1. 1.Zoological Survey of IndiaKolkataIndia
  2. 2.Department of BiologyPennsylvania State University, Shenango CampusSharonUSA

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