First Molecular Diagnosis of Lophomoniasis: the End of a Controversial Story
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We report a case of lophomoniasis in an immunocompetent patient with acute paranasal sinusitis from the north of Iran whose disease was diagnosed by both microscopic and molecular methods.
The patient, a 40-year-old woman, suffered from upper respiratory infection, was referred to the Iranian National Registry Center for Lophomoniasis (INRCL) at the Mazandaran University of Medical Sciences, Sari, Iran, for diagnosis. A direct wet mount of nasal discharge revealed the flagellate protozoa morphologically identified Lophomonas blattarum. Moreover, through a specific polymerase chain reaction (PCR) of nasal discharge, a 214-bp band was observed, confirming the genus Lophomonas spp. The patient was treated successfully with metronidazole 500 mg t.i.d for 1 week.
To the best of our knowledge, this is the first molecular detection of lophomoniasis in the literature. According to our preliminary study, a reliable PCR test is available now for detecting the Lophomonas parasite.
KeywordsLophomonas Human Sinusitis Molecular diagnosis
The authors wish to thank Mrs Amiri and Mrs Vafaei-Nezhad for kind contribution in INRCL.
Financial support for this study was received from the Mazandaran University of Medical Sciences, Sari, Iran (No: 1291).
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