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Acta Parasitologica

, Volume 64, Issue 3, pp 582–586 | Cite as

Occurrence of Anisakis pegreffii (Nematoda: Anisakidae) Larvae in Imported John Dory (Zeus faber) from Senegalese Coast Sold in Turkish Supermarkets

  • Gokmen Zafer PekmezciEmail author
Original Paper
  • 40 Downloads

Abstract

Background

West African goatfish Pseudupeneus prayensis, bluespotted seabream Pagrus caeruleostictus and John Dory Zeus faber are commercially marketed as fresh and frequently imported from Senegalese coast (FAO area 34.3.12) in Turkish supermarkets.

Purpose

The aim of the current study was to collect data of occurrence and molecular identification of Anisakis species in imported P. prayensis, P. caeruleostictus and Z. faber caught in the Senegalese coast and to support epidemiological report for a risk evaluation of Anisakis species in Turkish supermarkets.

Methods

Forty imported fish from each species at a total of 120 samples were investigated for the presence of Anisakis larvae. Based on ITS region of RFLP analysis Anisakis larvae were identified and randomly selected five larvae were also sequenced for further confirmation for cox2 gene.

Results

No Anisakis larvae were isolated from P. prayensis, P. caeruleostictus whereas Anisakis larvae were only found in Z. faber. A total of 156 Anisakis larvae were collected from Z. faber. All larvae were molecularly identified as Anisakis pegreffii. The prevalence (%), intensity and abundance of Anisakis infection in Z. faber were detected to be 82.5%, 8.3 and 6.8, respectively.

Conclusion

This is the first assessment of the occurrence of A. pegreffii in imported Z. faber from the Senegalese coast in Turkish supermarkets. Moreover, consuming imported P. prayensis and P. caeruleostictus present low to non-existent risk for anisakiasis in Turkish consumers. Furthermore, the presence of A. pegreffii larvae in imported Z. faber from the Senegal waters could have public health implications in Turkish consumers.

Keywords

Anisakis pegreffii Pseudupeneus prayensis Pagrus caeruleostictus Zeus faber Senegal Turkish supermarkets 

Notes

Acknowledgements

The author would like to thank DVM, PhD, Associate Professor Didem Pekmezci for English editing.

References

  1. 1.
    Alt KG, Kuhn T, Münster J, Klapper R, Kochmann J, Klimpel S (2018) Mesopredatory fishes from the subtropical upwelling region off NW-Africa characterised by their parasite fauna. PeerJ 6:e5339.  https://doi.org/10.7717/peerj.5339 CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410.  https://doi.org/10.1016/S0022-2836(05)80360-2 CrossRefPubMedGoogle Scholar
  3. 3.
    Arizono N, Yamada M, Tegoshi T, Yoshikawa M (2012) Anisakis simplex sensu stricto and Anisakis pegreffii: biological characteristics and pathogenetic potential in human anisakiasis. Foodborne Pathog Dis 9:517–521.  https://doi.org/10.1089/fpd.2011.1076 CrossRefPubMedGoogle Scholar
  4. 4.
    Audicana MT, Kennedy MW (2008) Anisakis simplex: from obscure infectious worm to inducer of immune hypersensitivity. Clin Microbiol Rev 21:360–379.  https://doi.org/10.1128/CMR.00012-07 CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Berland B (1961) Nematodes from some Norwegian marine fishes. Sarsia 2:1–50CrossRefGoogle Scholar
  6. 6.
    Bernardi C, Gustinelli A, Fioravanti ML, Caffara M, Mattiucci S (2011) Prevalence and mean intensity of Anisakis simplex (sensu stricto) in European sea bass (Dicentrarchus labrax) from Northeast Atlantic Ocean. Int J Food Microbiol 148:55–59.  https://doi.org/10.1016/j.ijfoodmicro.2011.04.027 CrossRefPubMedGoogle Scholar
  7. 7.
    Briguglio G, Di Caro G, Napoli E, Comignano F, Ferrantelli V, Gaglio G, Lanteri G (2017) Metazoan parasites of the John Dory Zeus faber Linnaeus, 1758 collected from the Mediterranean Sea. Cah Biol Mar 58:83–89.  https://doi.org/10.21411/cbm.a.4b08b2d9 CrossRefGoogle Scholar
  8. 8.
    Camara K, Carpenter KE, Djiman R, Nunoo F, Sagna A, Sidibé A, Sylla M, de Morais L, Quartey R, Lindeman K (2015) Pseudupeneus prayensis. The IUCN Red List of Threatened Species 2015:e.T18177478A42691864. http://dx.doi.org/10.2305/IUCN.UK.2015-4.RLTS.T18177478A42691864.en
  9. 9.
    D’ Amelio S, Mathiopoulos KD, Santos CP, Pugachev ON, Webb SC, Picanco M, Paggi L (2000) Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda: Ascaridoidea) defined by polymerase chain reaction-based restriction fragment length polymorphism. Int J Parasitol 30:223–226.  https://doi.org/10.1016/S0020-7519(99)00178-2 CrossRefGoogle Scholar
  10. 10.
    Food and Agriculture Organization of the United Nations (FAO) (2016) The State of World Fisheries and Aquaculture 2016. Contributing to Food Security and Nutrition for All, RomeCrossRefGoogle Scholar
  11. 11.
    Food and Agriculture Organization of the United Nations (FAO) (2018) http://www.fao.org/geonetwork/srv/eng/main.home?uuid=fao-fsa-map-34.3.12. Accessed 26 Oct 2018
  12. 12.
    Fumarola L, Monno R, Ierardi E, Rizzo G, Giannelli G, Lalle M, Pozio E (2009) Anisakis pegreffii etiological agent of gastric infections in two Italian women. Foodborne Pathog Dis 6:1157–1159.  https://doi.org/10.1089/fpd.2009.0325 CrossRefPubMedGoogle Scholar
  13. 13.
    Guardone L, Armani A, Nucera D, Costanzo F, Mattiucci S, Bruschi F (2018) Human anisakiasis in Italy: a retrospective epidemiological study over two decades. Parasite 25:41.  https://doi.org/10.1051/parasite/2018034 CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Ishikura H, Kikuchi K, Nagasawa K, Ooiwa T, Takamiya H, Sato N, Sugane K (1993) Anisakidae and anisakidosis. In: Sun T (ed) Progress in clinical parasitology, vol 3. Springer, New York, pp 43–102CrossRefGoogle Scholar
  15. 15.
    Iwamoto T (2015) Zeus faber. The IUCN Red List of Threatened Species 2015:e.T198769A42390771Google Scholar
  16. 16.
    Jovani R, Tella JL (2006) Parasite prevalence and sample size: misconceptions and solutions. Trends Parasitol 22(5):214–218.  https://doi.org/10.1016/j.pt.2006.02.011 CrossRefPubMedGoogle Scholar
  17. 17.
    Lim H, Jung BK, Cho J, Yooyen T, Shin EH, Chai JY (2015) Molecular diagnosis of cause of anisakiasis in humans, South Korea. Emerg Infect Dis 21:342–344.  https://doi.org/10.3201/eid2102.140798 CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Llarena-Reino M, Piñeiro C, Antonio J, Outeriño L, Vello C, González ÁF, Pascual S (2013) Optimization of the pepsin digestion method for anisakids inspection in the fishing industry. Vet Parasitol 191:276–283.  https://doi.org/10.1016/j.vetpar.2012.09.015 CrossRefPubMedGoogle Scholar
  19. 19.
    Marques JF, Cabral HN (2007) Effects of sample size on fish parasite prevalence, mean abundance and mean intensity estimates. J Appl Ichthyol 23:158–162.  https://doi.org/10.1111/j.1439-0426.2006.00823.x CrossRefGoogle Scholar
  20. 20.
    Mattiucci S, Cipriani P, Levsen A, Paoletti M, Nascetti G (2018) Molecular epidemiology of Anisakis and anisakiasis: an ecological and evolutionary road map. Adv Parasitol 99:93–263.  https://doi.org/10.1016/bs.apar.2017.12.001 CrossRefPubMedGoogle Scholar
  21. 21.
    Mattiucci S, Colantoni A, Crisafi B, Mori-Ubaldini F, Caponi L, Fazii P, Nascetti G, Bruschi F (2017) IgE sensitization to Anisakis pegreffii in Italy: comparison of two methods for the diagnosis of allergic anisakiasis. Parasite Immunol 39:e12440.  https://doi.org/10.1111/pim.12440 CrossRefGoogle Scholar
  22. 22.
    Mattiucci S, Fazii P, De Rosa A, Paoletti M, Salomone-Megna A, Glielmo A, De Angelis M, Costa A, Meucci C, Calvaruso V, Sorrentini I, Palma G, Bruschi F, Nascetti G (2013) Anisakiasis and gastroallergic reaction associated with Anisakis pegreffii infection, Italy. Emerg Infect Dis 19:496–499.  https://doi.org/10.3201/eid1903.121017 CrossRefPubMedPubMedCentralGoogle Scholar
  23. 23.
    Mattiucci S, Paoletti M, Borrini F, Palumbo M, Palmieri RM, Gomes V, Casati A, Nascetti G (2011) First molecular identification of the zoonotic parasite Anisakis pegreffii (Nematoda: Anisakidae) in a paraffin-embedded granuloma taken from a case of human intestinal anisakiasis in Italy. BMC Infect Dis 11:82–88.  https://doi.org/10.1186/1471-2334-11-82 CrossRefPubMedPubMedCentralGoogle Scholar
  24. 24.
    Mattiucci S, Paoletti M, De Angelis M, Sereno S, Cancrini G (2007) Human anisakidosis in Italy: molecular and histological identification of two new cases. Parassitologia 49:226Google Scholar
  25. 25.
    Mladineo I, Popović M, Drmić-Hofman I, Poljak V (2016) A case report of Anisakis pegreffii (Nematoda, Anisakidae) identified from archival paraffin sections of a Croatian patient. BMC Infect Dis 16:42–46.  https://doi.org/10.1186/s12879-016-1401-x CrossRefPubMedPubMedCentralGoogle Scholar
  26. 26.
    Nadler SA, Hudspeth DS (2000) Phylogeny of the Ascaridoidea (Nematoda: Ascaridida) based on three genes and morphology: hypotheses of structural and sequence evolution. J Parasitol 86:380–393.  https://doi.org/10.1645/0022-3395 CrossRefPubMedGoogle Scholar
  27. 27.
    Pekmezci GZ (2014) Occurrence of Anisakis simplex sensu stricto in imported Atlantic mackerel (Scomber scombrus) represents a risk for Turkish consumers. Int J Food Microbiol 185:64–68.  https://doi.org/10.1016/j.ijfoodmicro.2014.05.018 CrossRefPubMedGoogle Scholar
  28. 28.
    Pekmezci GZ, Onuk EE, Bolukbas CS, Yardimci B, Gurler AT, Acici M, Umur S (2014) Molecular identification of Anisakis species (Nematoda: Anisakidae) from marine fishes collected in Turkish waters. Vet Parasitol 201:82–94.  https://doi.org/10.1016/j.vetpar.2014.01.005 CrossRefPubMedGoogle Scholar
  29. 29.
    Pontes T, D’Amelio S, Costa G, Paggi L (2005) Molecular characterization of larval anisakid nematodes from marine fishes of Madeira by a PCR-based approach, with evidence for a new species. J Parasitol 91:1430–1434.  https://doi.org/10.1645/GE-565R1.1 CrossRefPubMedGoogle Scholar
  30. 30.
    Reiczigel J, Rózsa L (2005) Quantitative parasitology 3.0. Budapest. Distributed by the authorsGoogle Scholar
  31. 31.
    Rózsa L, Reiczigel J, Majoros G (2000) Quantifying parasites in samples of hosts. J Parasitol 86:228–232.  https://doi.org/10.1645/0022-3395 CrossRefPubMedGoogle Scholar
  32. 32.
    Russell B (2014) Pagrus caeruleostictus. The IUCN Red List of Threatened Species 2014:e.T170159A1284207. http://dx.doi.org/10.2305/IUCN.UK.2014-3.RLTS.T170159A1284207.en
  33. 33.
    Umehara A, Kawakami Y, Araki J, Uchida A (2007) Molecular identification of the etiological agent of the human anisakiasis in Japan. Parasitol Int 56:211–215.  https://doi.org/10.1016/j.parint.2007.02.005 CrossRefPubMedGoogle Scholar
  34. 34.
    Valentini A, Mattiucci S, Bondanelli P, Webb SC, Mignucci-Gianone A, Colom-Llavina M, Nascetti G (2006) Genetic relationships among Anisakis species (Nematoda: Anisakidae) inferred from mitochondrial cox-2 sequences, and comparisons with allozyme data. J Parasitol 92:156–166.  https://doi.org/10.1645/GE-3504.1 CrossRefPubMedGoogle Scholar
  35. 35.
    Valero A, Terrados S, Díaz V, Reguera V, Lozano J (2003) Determination of IgE in the serum of patients with allergic reactions to four species of fish-parasite anisakids. J Investig Allergol Clin Immunol 13:94–98PubMedGoogle Scholar
  36. 36.
    Vassiliades G (1985) Helminthes parasites des poissons de mer des côtes du Sénégal. Bull IFAN Ser B 44:78–99Google Scholar
  37. 37.
    Yera H, Fréalle E, Dupouy-Camet J (2016) Molecular confirmation of Anisakis pegreffii as a causative agent of anisakidosis in France. Dig Liver Dis 48:970–971.  https://doi.org/10.1016/j.dld.2016.04.003 CrossRefPubMedGoogle Scholar
  38. 38.
    Zhu X, Gasser RB, Podolska M, Chilton NB (1998) Characterization of anisakid nematodes with zoonotic potential by nuclear ribosomal DNA sequences. Int J Parasitol 28:1911–1921.  https://doi.org/10.1016/S0020-7519(98)00150-7 CrossRefPubMedGoogle Scholar

Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2019

Authors and Affiliations

  1. 1.Department of Aquatic Animal Diseases, Faculty of Veterinary MedicineOndokuz Mayis UniversitySamsunTurkey

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