Ceratomyxa Thélohan, 1892 is one of the largest genera under Myxosporea Butschli, 1881, and has a worldwide distribution, but little attention has been paid to myxosporean parasites from the Chinese seawaters, East China Sea.
Morphology and molecular biology methods were combined for species identification and phylogenetic analysis.
A new coelozoic myxosporean species, Ceratomyxa siganicola n. sp., was found to infect the gallbladder of Siganus fuscescens (Houttuyn, 1782) (Perciformes, Siganidae) from coastal waters of Xiamen, East China Sea, China. Mature myxospores of the novel species exhibited the morphologically typical features of the genus Ceratomyxa. They were slightly crescent shaped with rounded ends, measuring 5.6 ± 0.5 (4.8–6.5) μm in length and 19.1 ± 1.8 (16.0–22.1) μm in thickness. The posterior angle was slightly convex to straight and measured 177.1 ± 0.5 (175.2–178.4)°. Spore valves were slightly unequal and smoothly ovoid in the lateral view. Two polar capsules were spherical, equal in size and measured 2.7 ± 0.2 (2.1–3.0) μm in diameter. The 18S rDNA sequence of C. siganicola n. sp. was unique among all myxozoans, and the highest similarity was 97.4% with Ceratomyxa barnesi. Phylogenetic analysis showed that C. siganicola n. sp. was clustered within the clade of siganid ceratomyxids. The present results also indicated that the species radiation of Ceratomyxa occurred not only within host affinity but also within locality.
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Gunter NL, Adlard RD (2010) The demise of Leptotheca Thélohan, 1895 (Myxozoa: Myxosporea: Ceratomyxidae) and assignment of its species to Ceratomyxa Thelohan, 1892 (Myxosporea: Ceratomyxidae), Ellipsomyxa Køie, 2003 (Myxosporea: Ceratomyxidae), Myxobolus Butschli, 1882 and Sphaerospora Thélohan, 1892 (Myxosporea: Sphaerosporidae). Syst Parasitol 75:81–104. https://doi.org/10.1007/s11230-009-9227-1
Sanil NK, Chandran A, Shamal P, Binesh CP (2017) Molecular and morphological descriptions of Ceratomyxa collarae n. sp. and Ceratomyxa leucosternoni n. sp. from marine ornamental fishes of Indian waters. Parasitol Res 116:1–10. https://doi.org/10.1007/s00436-016-5317-6
Eiras JC (2006) Synopsis of the species of the genus Ceratomyxa Thélohan, 1892 (Myxozoa: Myxosporea: Ceratomyxidae). Syst Parasitol 65:49–71. https://doi.org/10.1007/s11230-006-9039-5
Zhao YJ, Al-Farraj SA, AL-Rasheid KAS, Song WB (2015) Data on ten new myxosporean parasites (Myxozoa, Myxosporea, Bivalvulida) from the Yellow Sea, China. Acta Protozoologica 54:305–323. https://doi.org/10.4467/16890027ap.15.026.3540
Lom J, Dyková I (1992) Protozoans parasites of fishes. Developments in Aquaculture and Fisheries Science 26:1–315
Bartošová P, Fiala I, Hypša V (2009) Concatenated SSU and LSU rDNA data confirm the main evolutionary trends within myxosporeans (Myxozoa: Myxosporea) and provide an effective tool for their molecular phylogenetics. Mol Phylogenet Evol 53:81–93. https://doi.org/10.1016/j.ympev.2009.05.018
Fiala I (2006) The phylogeny of Myxosporea (Myxozoa) based on small subunit ribosomal RNA gene analysis. Int J Parasitol 36:1521–1534. https://doi.org/10.1016/j.ijpara.2006.06.016
Holzer AS, Sommerville C, Wootten R (2004) Molecular relationships and phylogeny in a community of myxosporeans and actinosporeans based on their 18S rDNA sequences. Int J Parasitol 34:1099–1111. https://doi.org/10.1016/j.ijpara.2004.06.002
Kent ML, Andree KB, Bartholomew JL, El-Matbouli M, Desser SS, Devlin RH et al (2001) Recent advances in our knowledge of the Myxozoa. J Eukaryot Microbiol 48:395–413. https://doi.org/10.1111/j.1550-7408.2001.tb00173.x
Yang CZ, Zhou Y, Zhao YJ, Huang W, Huang C (2017) Erection of Unicapsulocaudum mugilum gen. et sp. nov. (Myxozoa: Ceratomyxidae) based on its morphological and molecular data. Journal of Natural History 51:457–467. https://doi.org/10.1080/00222933.2017.1303096
Zhao YJ, Zhou Y, Kent ML, Whipps CM (2008) Replacement of the preoccupied name Davisia Laird 1953 and description of a new myxozoan species (Myxosporea: Sinuolineidae) from Sebastiscus marmoratus (Cuvier, 1829) in the East China Sea. J Parasitol 94:269–279. https://doi.org/10.1645/ge-1232.1
Zhou Y, Zhao YJ (2008) Descriptions of three new species of Kudoa (Myxosporea, Multivalvulida, Kudoidae) parasitic in marine fishes from the East China Sea. Acta Hydrobiol Sin 32:51–56. https://doi.org/10.3724/sp.j.0000.2008.70051
Zhao YJ, Ma CL, Song WB (2001) Illustrated guide to the identification of pathogenetic protozoa in maricutureII Diagnostic methods for the myxosporean. J Ocean Univ Qingdao 31:681–688. https://doi.org/10.16441/j.cnki.hdxb.2001.05.009
Hillis DM, Dixon MT (1991) Ribosomal DNA: molecular evolution and phylogenetic inference. Quarterly Review of Biology 66:411–453. https://doi.org/10.1086/417338
Whipps CM, Adlard RD, Bryant MS, Lester RJG, Findlay V, Kent ML (2003) The first report of three Kudoa species from Eastern Australia: Kudoa thyrsites from Mahi mahi (Coryphaena hippurus), Kudoa amamiensis and Kudoa minithyrsites n. sp. from Sweeper (Pempheris ypsilychnus). J Eukaryot Microbiol 50:215–219. https://doi.org/10.1111/j.1550-7408.2003.tb00120.x
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DJ (1997) The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882. https://doi.org/10.1093/nar/25.24.4876
Castresana J (2000) Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol 17:540–552. https://doi.org/10.1093/oxfordjournals.molbev.a026334
Ronquist F, Huelsenbeck JP (2003) MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574. https://doi.org/10.1093/bioinformatics/btg180
Posada D, Crandall KA (1998) Modeltest: testing the model of DNA substitution. Bioinformatics 4:817–818. https://doi.org/10.1093/bioinformatics/14.9.817
Rambaut A, Drummond AJ (2005) Tracer V1.3: MCMC trace analysis tool. Institute of evolutionary biology [internet]. Edinburgh: University of Edinburgh. Available at: http://tree.bio.ed.ac.uk/software/tracer/
Stamatakis A, Hoover P, Rougemont J (2008) A rapid bootstrap algorithm for the RAxMLWeb servers. Syst Biol 75:758–771. https://doi.org/10.1080/10635150802429642
Fiala I, Hlavnickova M, Kodadkova A, Freeman MA, Bartošova-Sojkova P, Atkinson SD (2015) Evolutionary origin of Ceratonova shasta and phylogeny of the marine myxosporean lineage. Mol Biol Evol 86:75–89. https://doi.org/10.1016/j.ympev.2015.03.004
Heiniger H, Cribb TH, Adlard RD (2013) Intra-specific variation of Kudoa spp. (Myxosporea: Multivalvulida) from apogonid fishes (Perciformes), including the description of two new species, K. cheilodipteri n. sp. and K. cookii n. sp., from Australian waters. Syst Parasitol 84:193–215. https://doi.org/10.1007/s11230-012-9400-9
Heiniger H, Gunter NL, Adlard RD (2008) Relationships between four novel ceratomyxid parasites from the gall bladders of labrid fishes from Heron Island, Queensland, Australia. J Parasitol 57:158–165. https://doi.org/10.1016/j.parint.2007.11.006
Morsy K, Abdel-Ghaffar F, Bashtar AR, Mehlhorn H, Al Quraishy S, Abdel-Gaber R (2012) Morphology and small subunit ribosomal DNA sequence of Henneguya suprabranchiae (Myxozoa), a parasite of the catfish Clarias gariepinus (Clariidae) from the River Nile. Egypt. Parasitology Research 111:1423–1435. https://doi.org/10.1007/s00436-012-2976-9
Gunter NL, Adlard RD (2009) Seven new species of Ceratomyxa Thélohan, 1892 (Myxozoa) from the gall-bladders of serranid fishes from the Great Barrier Reef, Australia. Syst Parasitol 73:1–11. https://doi.org/10.1007/s11230-008-9162-6
Gunter NL, Whipps CM, Adlard RD (2009) Ceratomyxa (Myxozoa: Bivalvulida): robust taxon or genus of convenience? Int J Parasitol 39:1395–1405. https://doi.org/10.1016/j.ijpara.2009.04.008
Heiniger H, Adlard RD (2013) Molecular identification of cryptic species of Ceratomyxa Thélohan, 1892 (Myxosporea: Bivalvulida) including the description of eight novel species from apogonid fishes (Perciformes: Apogonidae) from Australian waters. Acta Parasitologica 58:342–360. https://doi.org/10.2478/s11686-013-0149-3
Alama-Bermejo G, Raga JA, Holzer AS (2011) Host-parasite relationship of Ceratomyxa puntazzi n. sp. (Myxozoa: Myxosporea) and sharpsnout seabream Diplodus puntazzo (Walbaum, 1792) from the Mediterranean with first data on ceratomyxid host specificity in sparids. Vet Parasitol 182:181–192. https://doi.org/10.1016/j.vetpar.2011.05.012
Lubat V, Radujkovic B, Marques A, Bouix G (1989) Parasites des poissons marins du Montenegro: Myxosporidies. Acta Adriat 30:31–50
Moser M, Kent ML, Dennis D (1989) Gall-bladder Myxosporea in Coral-Reef fishes from Heron Island, Australia. Australian Journal of Zoology 37:1–13. https://doi.org/10.1071/zo9890001
Gunter NL, Adlard RD (2008) Bivalvulidan (Myxozoa: Myxosporea) parasites of damselfishes with description of twelve novel species from Australia’s Great Barrier Reef. Parasitology 135:1165–1178. https://doi.org/10.1017/s0031182008004733
This work was supported by grants from the National Natural Science Foundation of China (nos. 31501845, 31672280 and 31471980), project of Chongqing Science and Technology Commission (nos. cstc2017jcyjAX0165, cstc2018jcyjAX0738) and Venture and Innovation Support Program for Chongqing Overseas Returnees (cx2018108).
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Zhang, D., Zhao, Y., Yang, S. et al. Morphological and Molecular Identification of a Novel Species, Ceratomyxa siganicola n. sp. (Myxozoa: Ceratomyxidae) from Siganus fuscescens, in East China Sea. Acta Parasit. 64, 596–602 (2019). https://doi.org/10.2478/s11686-019-00077-1
- Ceratomyxa siganicola n. sp.
- Siganus fuscescens
- Molecular phylogeny
- 18S rDNA