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Acta Parasitologica

, Volume 64, Issue 3, pp 566–574 | Cite as

The Anthelmintic Effects of Medicinal Plant Extracts Against Paramphistome Parasites, Carmyerius spatiosus

  • Sutthida Minsakorn
  • Khwannapa Nuplod
  • Panupong Puttarak
  • Runglawan Chawengkirttikul
  • Busaba Panyarachun
  • Arin Ngamniyom
  • Tanapat Charoenkul
  • Methasit Jaisa-aad
  • Panadda Panyarachun
  • Panat AnuracpreedaEmail author
Original Paper
  • 88 Downloads

Abstract

Introduction

Paramphistomosis is a disease caused by the rumen flukes which cause an acute gastroenteritis and anemia with high mortality particularly in young ruminants.

Materials and methods

In this study, we have investigated the anthelmintic effect of medicinal plant extracts from leaves and heartwoods of Cassia siamea L., roots of Plumbago zeylanica L. and Plumbago indica L., and leaves of Terminalia catappa L. against Carmyerius spatiosus.

Results

The highest anthelminthic effect on the flukes after 24 h of exposure was found in heartwood ethyl acetate extract of C. siamea (LC50 = 374.30; LC90 = 749.03 ppm), root n-butanol extract of P. zeylanica (LC50 = 1005.12; LC90 = 2411.55 ppm), root hexane, ethyl acetate, and n-butanol extract of P. indica (LC50 = 34.38, 211.34, 506.92; LC90 = 64.09, 496.05, 934.86 ppm), and leaf n-butanol and water extract of T. catappa (LC50 = 487.17, 470.28; LC90 = 913.27, 848.23 ppm). When observed by scanning electron microscopy, the tegument showed similar sequence of morphological changes after treatments with all plant extracts, comprising of swelling of ridges and folds, blebbing, rupturing of the blebs, erosion, lesion and disruption of the tegument.

Conclusion

This study is the first report on the anthelmintic activity of plant extracts to C. spatiosus; therefore, these plant extracts are highly effective in the elimination of adult rumen flukes.

Keywords

Medicinal plant extracts Carmyerius spatiosus Anthelmintic drug Tegument Scanning electron microscopy 

Notes

Acknowledgements

This work was financially supported by Research Grants from Agricultural Research Development Agency (ARDA), National Research Council of Thailand (NRCT) and TRF Research Career Development Grant co-funded by The Thailand Research Fund and Mahidol University (RSA6080071) to Panat Anuracpreeda.

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Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2019

Authors and Affiliations

  • Sutthida Minsakorn
    • 1
  • Khwannapa Nuplod
    • 2
  • Panupong Puttarak
    • 2
  • Runglawan Chawengkirttikul
    • 3
  • Busaba Panyarachun
    • 4
  • Arin Ngamniyom
    • 5
  • Tanapat Charoenkul
    • 4
  • Methasit Jaisa-aad
    • 4
  • Panadda Panyarachun
    • 6
  • Panat Anuracpreeda
    • 7
    Email author
  1. 1.Faculty of Veterinary TechnologyKasetsart UniversityBangkokThailand
  2. 2.Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical SciencesPrince of Songkla UniversitySongkhlaThailand
  3. 3.Department of Microbiology, Faculty of ScienceMahidol UniversityBangkokThailand
  4. 4.Department of Anatomy, Faculty of MedicineSrinakharinwirot UniversityBangkokThailand
  5. 5.Faculty of Environmental Culture and EcotourismSrinakharinwirot UniversityBangkokThailand
  6. 6.Department of Opthalmology, HRH Princess Maha Chakri Sirindhorn Medical Center, Faculty of MedicineSrinakharinwirot UniversityNakhon NayokThailand
  7. 7.Institute of Molecular BiosciencesMahidol UniversityNakhon PathomThailand

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