Acta Parasitologica

, Volume 64, Issue 3, pp 645–651 | Cite as

A Laboratory Strain of Leishmania major: Protective Effects on Experimental Leishmaniasis

  • Mehdi Namavari
  • Fatemeh NamaziEmail author
  • Reza Asadi-Manesh
  • Mohammad H. Hosseini
  • Saeed Nazifi
  • Mohammad Asadpour
Original Paper



Leishmaniasis, as one of the most important vector-borne and zoonotic diseases, can be seen in different forms and is more prevalent in developing countries worldwide. Due to the absence of effective strategies in its prevention, treatment, and control, investigation of effective control strategies against the disease is necessary. In this research, we evaluated the immunogenicity of a cold-adapted laboratory strain of Leishmania major (LMC) in the mouse model.


Twenty BALB/c mice were divided into two groups. LMC group received 4 × 106 of LMC strain in 0.5 ml DMEM, and VLM group, as the control group, received 0.5 ml Dulbecco’s modified Eagle’s medium. Both groups were challenged with virulent L. major 3 weeks after inoculation.


The data obtained from the analysis of immune responses and histopathological changes interestingly revealed protection against L. major in immunized mice. Compared with the VLM group, the mice immunized with LMC strain of L. major in the LMC group showed a significant increase in IFN-γ and IgG2a levels (P < 0.05) which are important indexes for Th1-related immune responses. Additionally, significant differences in concentration of IgG1 and IgG total before and after the challenge was observed in LMC group (P < 0.05). Furthermore, the immunized mice showed a significant reduction in mean sizes of skin lesion and liver damage compared to the VLM group.


Based on the present findings on immunogenicity of LMC strain, it seems this strain is able to induce both humoral and cellular immunity and a significant protection against L. major in the mouse model.


Leishmania major Cold-adapted laboratory strain Immunogenicity Histopathology BALB/c mice 



This work was supported by the Research Council of Shiraz University and School of Veterinary Medicine, Shiraz University (94GCU5M83832) and the Razi Vaccine and Serum Research Institute, Shiraz Branch, Agricultural Research, Education and Extension Organization (AREEO).

Compliance with Ethical Standards

Conflict of Interest

The authors have no competing interests.


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Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2019

Authors and Affiliations

  • Mehdi Namavari
    • 1
  • Fatemeh Namazi
    • 2
    Email author
  • Reza Asadi-Manesh
    • 3
  • Mohammad H. Hosseini
    • 1
  • Saeed Nazifi
    • 4
  • Mohammad Asadpour
    • 2
  1. 1.Razi Vaccine and Serum Research Institute, Shiraz BranchAgricultural Research, Education and Extension Organization (AREEO)ShirazIran
  2. 2.Department of Pathobiology, School of Veterinary MedicineShiraz UniversityShirazIran
  3. 3.School of Veterinary MedicineShiraz UniversityShirazIran
  4. 4.Department of Clinical Sciences, School of Veterinary MedicineShiraz UniversityShirazIran

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