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Acta Parasitologica

, Volume 64, Issue 3, pp 442–448 | Cite as

Morphological and Genetic Characterization of the Poorly Known Species Subulura chinensis Schwartz, 1926 (Nematoda: Ascaridida) from Athene noctua (Scopoli) (Strigiformes: Strigidae)

  • Ning Guo
  • Lu-Ping Zhang
  • Lin-Wei Li
  • Liang LiEmail author
Original Paper

Abstract

Introduction

Subulura chinensis Schwartz, 1926 is a hitherto poorly known nematode species. The morphology of S. chinensis has not been sufficiently well described. In addition, the molecular data from species of the Subuluroidea are extremely limited.

Methods

The detailed morphology of S. chinensis was studied using light microscopy and, for the first time, scanning electron microscopy, based on newly collected specimens from the little owl Athene noctua (Scopoli) (Strigiformes: Strigidae) in China. The internal transcribed spacer (ITS) of ribosomal DNA and mitochondrial cytochrome c oxidase subunit 1 (cox1) target regions of S. chinensis were first amplified by polymerase chain reaction (PCR), then sequenced and analysed for the molecular identification of this species.

Results

Our SEM observations showed for the fist time the detailed morphology of the cephalic extremity, precloacal pseudo-sucker, caudal papillae, gubernaculum, phasmids and vulva of S. chinensis, and also determined the presence of a small, single medio-ventral precloacal papilla in the male. Moreover, we detected the presence of 0.08–0.40% and 0–1.30% nucleotide divergence among different individuals of S. chinensis in the ITS and cox1 regions, respectively. The supplementary morphological characters and genetic data will be very useful for the diagnosis of this poorly known species.

Keywords

Parasite Nematoda Bird Subuluroidea Strigiformes Redescription SEM Molecular data 

Notes

Acknowledgements

This study was supported by the National Natural Science Foundation of China (NSFC-31572231) and the Youth Top Talent Support Program of Hebei Province for Liang Li.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2019

Authors and Affiliations

  1. 1.Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life SciencesHebei Normal UniversityShijiazhuangPeople’s Republic of China

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