Susceptibility to Various Coccidiostats in the Murine Coccidian Parasite Eimeria krijgsmanni
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Murine Eimeria spp. have been used as effective experimental models of disease instead of large mammalian hosts such as cattle. We here examine drug susceptibility of the uncharacterized murine intestinal protozoan parasite, Eimeria krijgsmanni.
Materials and methods
The effectiveness of different treatments against infection of E. krijgsmanni was examined for suppression of oocyst shedding: ST mixture ST mixture, pyrimethamine, Ektecin and toltrazuril.
ST mixture and pyrimethamine did not suppress oocyst shedding effectively. Although therapeutic efficacy of Ektecin was demonstrated, the dose required was larger than that for cattle and chickens. Oocyst shedding was only completely suppressed completely by continuous administration of toltrazuril. Furthermore, it was confirmed through morphological examination that early developmental stage zoites appeared in host epithelial cells during and following treatment by toltrazuril, and toltrazuril could not eliminate residual zoites in epithelial cells.
E. krijgsmanni may be relatively resistant to these anti-coccidian agents and might therefore have different characteristics that differ from other coccidia with regard to drug susceptibility.
KeywordsCoccidium Mouse Sulfa drug Toltrazuril Pyrimethamine Eimeria
This work was partly supported by Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (Nos. 16K08025 and 16H05803).
- 1.Aponte JJ, Schellenberg D, Egan A, Breckenridge A, Carneiro I, Critchley J, Danquah I, Dodoo A, Kobbe R, Lell B, May J, Premji Z, Sanz S, Sevene E, Soulaymani-Becheikh R, Winstanley P, Adjei S, Anemana S, Chandramohan D, Issifou S, Mockenhaupt F, Owusu-Agyei S, Greenwood B, Grobusch MP, Kremsner PG, Macete E, Mshinda H, Newman RD, Slutsker L, Tanner M, Alonso P, Menendez C (2009) Efficacy and safety of intermittent preventive treatment with sulfadoxine-pyrimethamine for malaria in African infants: a pooled analysis of six randomised, placebo-controlled trials. Lancet 374:1533–1542. https://doi.org/10.1016/S0140-6736(09)61258-7 CrossRefPubMedGoogle Scholar
- 12.Inagaki-Ohara K, Dewi FN, Hisaeda H, Smith AL, Jimi F, Miyahira M, Abdel-Aleem AS, Horii Y, Nawa Y (2006) Intestinal intraepithelial lymphocytes sustain the epithelial barrier function against Eimeria vermiformis infection. Infect Immun 74:5292–5301. https://doi.org/10.1128/IAI.02024-05 CrossRefPubMedPubMedCentralGoogle Scholar
- 13.Jonsson NN, Piper EK, Gray CP, Deniz A, Constantinoiu CC (2011) Efficacy of toltrazuril 5% suspension against Eimeria bovis and Eimeria zuernii in calves and observations on the associated immunopathology. Parasitol Res 109:S113–S128. https://doi.org/10.1007/s00436-010-2129-y CrossRefPubMedGoogle Scholar
- 15.Levine MD (1982) Taxonomy and life cycles of coccidia. In: Long PL (ed) The biology of the coccidia. University Park Press, Baltimore, pp 1–33Google Scholar
- 18.Mehlhorn H (ed) (2008) Encyclopedic reference of parasitology, vol 1, 3rd edn. Springer, BerlinGoogle Scholar
- 22.Philippe P, Alzieu JP, Taylor MA, Dorchies P (2014) Comparative efficacy of diclazuril (Vecoxan®) and toltrazuril (Baycox bovis®) against natural infections of Eimeria bovis and Eimeria zuernii in French calves. Vet Parasitol 206:129–137. https://doi.org/10.1016/j.vetpar.2014.10.003 CrossRefPubMedGoogle Scholar
- 23.Schmid M, Heitlinger E, Spork S, Mollenkopf HJ, Lucius R, Gupta N (2014) Eimeria falciformis infection of the mouse caecum identifies opposing roles of IFNγ-regulated host pathways for the parasite development. Mucosal Immunol 7:969–982. https://doi.org/10.1038/mi.2013.115 CrossRefPubMedGoogle Scholar
- 24.Takeo T, Tanaka T, Matsubayashi M, Maeda H, Kusakisako K, Matsui T, Mochizuki M, Matsuo T (2014) Molecular and phylogenetic characterizations of an Eimeria krijgsmanni Yakimoff & Gouseff, 1938 (Apicomplexa: Eimeriidae) mouse intestinal protozoan parasite by partial 18S ribosomal RNA gene sequence analysis. Parasitol Int 63:627–630. https://doi.org/10.1016/j.parint.2014.04.004 CrossRefPubMedGoogle Scholar
- 25.Takeo T, Tanaka T, Matsubayashi M, Tsujio M, Umemiya-Shirafuji R, Tsuji N, Fujisaki K, Matsui T, Matsuo T (2015) Evaluation of Eimeria krijgsmanni as a murine model for testing the efficacy of anti-parasitic agents. Acta Parasitol 60:190–195. https://doi.org/10.1515/ap-2015-0027 CrossRefPubMedGoogle Scholar
- 26.Verdier RI, Fitzgerald DW, Johnson WD Jr, Pape JW (2000) Trimethoprim-sulfamethoxazole compared with ciprofloxacin for treatment and prophylaxis of Isospora belli and Cyclospora cayetanensis infection in HIV-infected patients. A randomized, controlled trial. Ann Intern Med 132:885–888CrossRefPubMedGoogle Scholar