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Susceptibility to Various Coccidiostats in the Murine Coccidian Parasite Eimeria krijgsmanni

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Abstract

Introduction

Murine Eimeria spp. have been used as effective experimental models of disease instead of large mammalian hosts such as cattle. We here examine drug susceptibility of the uncharacterized murine intestinal protozoan parasite, Eimeria krijgsmanni.

Materials and methods

The effectiveness of different treatments against infection of E. krijgsmanni was examined for suppression of oocyst shedding: ST mixture ST mixture, pyrimethamine, Ektecin and toltrazuril.

Results

ST mixture and pyrimethamine did not suppress oocyst shedding effectively. Although therapeutic efficacy of Ektecin was demonstrated, the dose required was larger than that for cattle and chickens. Oocyst shedding was only completely suppressed completely by continuous administration of toltrazuril. Furthermore, it was confirmed through morphological examination that early developmental stage zoites appeared in host epithelial cells during and following treatment by toltrazuril, and toltrazuril could not eliminate residual zoites in epithelial cells.

Conclusion

E. krijgsmanni may be relatively resistant to these anti-coccidian agents and might therefore have different characteristics that differ from other coccidia with regard to drug susceptibility.

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Acknowledgements

This work was partly supported by Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (Nos. 16K08025 and 16H05803).

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Authors and Affiliations

  1. Laboratory of Parasitology, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, 890-0065, Japan

    Kota Inoue, Ryota Inoue, Keisuke Abe & Tomohide Matsuo

  2. Laboratory of Anatomy, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, 890-0065, Japan

    Masashi Tsujio

  3. Laboratory of International Prevention of Epidemics, Department of Veterinary Science, Graduate School of Life and Environmental Science, Osaka Prefecture University, Izumisano, Osaka, 598-8531, Japan

    Makoto Matsubayashi

  4. Laboratory of Veterinary Histopathology, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, 890-0065, Japan

    Hitoshi Hatai

  5. Laboratory of Public Health, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, 890-0065, Japan

    Masako Andoh

  6. Seisen University, Higashi Gotanda, Tokyo, 141-8642, Japan

    Toshihiro Matsui

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  1. Kota Inoue
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Correspondence to Tomohide Matsuo.

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Inoue, K., Tsujio, M., Matsubayashi, M. et al. Susceptibility to Various Coccidiostats in the Murine Coccidian Parasite Eimeria krijgsmanni. Acta Parasit. 64, 418–422 (2019). https://doi.org/10.2478/s11686-019-00052-w

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