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Sequence Analysis of the cAMP-Dependent Protein Kinase Regulatory Subunit-Like Protein From Trypanosoma brucei

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Abstract

Purpose

Study the N-terminal, C-terminal, and linker regions of the TbPKAr using homology modeling.

Methods

The amino acid sequences of the N-terminal, C-terminal, and linker regions of the TbPKAr were individually examined by means of BLAST analysis and in silico secondary structure predictions with several programs.

Results

The TbPKAr C-terminal region, showed a well-folded α/β structure, which consists of two concurrent flattened β-barrel-shaped domains that are separated by an elongated central α-helix similar to its mammalian counterpart, the TbPKAr linker region contains a PKA phosphorylation site and was predicted to be rather disordered. Our analysis also indicated that the TbPKAr N-terminal region lacks a docking/dimerization domain but is enriched in motifs known as leucine-rich repeats (LRR).

Conclusion

The replacement of the docking/dimerization domain by different structural motifs suggests the inability of TbPKAr to form homodimers; however, the function of the TbPKAr N-terminal LRR-containing domain in Kinetoplastidae parasites is still unknown.

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Acknowledgements

The authors thank Juan Ricardo Rodrigues for his very useful comments and for reviewing an initial draft of the manuscript. This research was supported by Grant numbers S1-IN-CB-002-17 from Decanato de Investigación y Desarrollo, Universidad Simón Bolívar, Caracas, Venezuela, and 2013001659 from FONACIT, Caracas, Venezuela.

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Authors and Affiliations

  1. Laboratorio de Caracterización de Proteínas, Departamento de Biología Celular, Universidad Simón Bolívar, Apartado 89.000, Caracas, 1081-A, Venezuela

    Nelson A. Araujo

  2. Laboratorio de Química de Proteínas, Departamento de Biología Celular, Universidad Simón Bolívar, Apartado 89.000, Caracas, 1081-A, Venezuela

    José Bubis

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  1. Nelson A. Araujo
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  2. José Bubis
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Correspondence to Nelson A. Araujo.

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Araujo, N.A., Bubis, J. Sequence Analysis of the cAMP-Dependent Protein Kinase Regulatory Subunit-Like Protein From Trypanosoma brucei. Acta Parasit. 64, 262–267 (2019). https://doi.org/10.2478/s11686-019-00037-9

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