Acta Parasitologica

, Volume 64, Issue 2, pp 251–256 | Cite as

The Construction of Full-Length cDNA Library for Otodectes cynotis

  • Li Hu
  • Ya-e ZhaoEmail author
  • Dong-ling Niu
  • Rui Yang
  • Ji-hui Zeng
Original Paper



Otodectes cynotis (Hering, 1838) is the pathogen of otodectic mange distributed worldwide. The mite mainly infests carnivores and, sometimes, humans. However, due to the lack of cDNA library, research on its pathogenesis has been challenging.


To solve this problem, the present study first sampled O. cynotis mites from an infested cat from Xi’an, China, for RNA extraction. Then, the full-length cDNA library was constructed using the SMART technique. Finally, positive clones > 500 bp and Hsc70-5 gene fragment specifically amplified from the cDNA library were sequenced and analyzed to verify the library’s reliability.


Results showed that RNA extracted from 300 mites had good quality with a concentration of 149 ng/μl and OD260/OD280 of 1.99. The library satisfied the quality standard of a good library with a titer of 5.02 × 105 PFU/ml and a combination rate of 97.61%. In addition, clone 4 and Hsc70-5 showed 98.38% and 99.72% identity with Ef1-α and Hsc70-5 gene sequences of O. cynotis in GenBank, respectively.


The cDNA library of O. cynotis constructed here was successful and reliable, creating the basis for research on RNA sequencing and functional genes of O. cynotis.


Otodectic mange cDNA library Quality evaluation Specific primer detection 



This work was supported by the National Natural Science Foundation of China (Nos. 81471972; 81271856).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11686_2019_34_MOESM1_ESM.docx (21 kb)
Supplementary material 1 (DOCX 21 kb)
11686_2019_34_MOESM2_ESM.jpg (393 kb)
Supplementary Fig. 1. Sequence analysis of Ef1-α gene. A. Sequence alignments of Otodectes, B. ML tree across Psoroptidia. (JPEG 392 kb)
11686_2019_34_MOESM3_ESM.jpg (294 kb)
Supplementary Fig. 2. Sequence analysis of Hsc70-5 gene. A. Sequence alignments of Otodectes, B. ML tree across Psoroptidia. (JPEG 294 kb)


  1. 1.
    Ahn A.J., Oh D.S., Ahn K.S., Shin S.S. 2013. First feline case of otodectosis in the Republic of Korea and successful treatment with imidacloprid/moxidectin topical solution. The Korean Journal of Parasitology, 51, 125–128. CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Arther R.G., Davis W.L., Jacobsen J.A., Lewis V.A., Settje T.L. 2015. Clinical evaluation of the safety and efficacy of 10% imidacloprid + 2.5% moxidectin topical solution for the treatment of ear mite (Otodectes cynotis) infestations in dogs. Veterinary Parasitology, 210, 64–68. CrossRefPubMedGoogle Scholar
  3. 3.
    Beugnet F., Bourdeau P., Chalvet-Monfray K., Cozma V., Farkas R., Guillot J., Halos L., Joachim A., Losson B., Miró G., Otranto D., Renaud M., Rinaldi L. 2014. Parasites of domestic owned cats in Europe: co-infestations and risk factors. Parasites & Vectors, 7, 291. CrossRefGoogle Scholar
  4. 4.
    Blot C., Kodjo A., Reynaud M.C., Bourdoiseau G. 2003. Efficacy of selamectin administered topically in the treatment of feline otoacariosis. Veterinary Parasitology, 112, 241–247. Scholar
  5. 5.
    Cheng J., Liu C.C., Zhao Y.E., Hu L., Yang Y.J., Yang F., Shi Z.Y. 2015. Population identification and divergence threshold in Psoroptidae based on ribosomal ITS2 and mitochondrial COI genes. Parasitology Research, 114, 3497–3507. CrossRefGoogle Scholar
  6. 6.
    Davis W.L., Arther R.G., Settje T.S. 2007. Clinical evaluation of the efficacy and safety of topically applied imidacloprid plus moxidectin against ear mites (Otodectes cynotis) in client-owned cats. Parasitology Research, 101, S19–S24. CrossRefGoogle Scholar
  7. 7.
    Fischer K., Holt D.C., Haruma P., Currie B.J., Walton S.F., Kem D.J. 2003. Generation and characterization of cDNA clones from Sarcoptes scabiei var. hominis for an expressed sequence tag library: identification of homologues of house dust mite allergens. The American Journal of Tropical Medicine and Hygiene, 68, 61–64.CrossRefPubMedGoogle Scholar
  8. 8.
    Hu L., Zhao Y.E., Cheng J., Yang Y.J., Li C., Lu Z.H. 2015. Constructing and detecting a cDNA library for mites. Parasitology Research, 114, 3893–3901. CrossRefPubMedGoogle Scholar
  9. 9.
    Kato T., Ito Y., Nakao H., Kadosaka T. 2011. Tinnitus caused by tiny mites-suspected cases of erratic migration of Otodectes cynotis and a case of Aleuroglyphus ovatus infestation. Medical Entomology and Zoology, 62, 199–204.CrossRefGoogle Scholar
  10. 10.
    Kenyon F., Welsh M., Parkinson J., Whitton C., Blaxter M.L., Knox D.P. 2003. Expressed sequence tag survey of gene expression in the scab mite Psoroptes ovis–allergens, proteases and free-radical scavengers. Parasitology, 126, 451–460. CrossRefPubMedGoogle Scholar
  11. 11.
    Lee A.J., Elwyn Isaac R., Coates D. 1999. The construction of a cDNA expression library for the sheep scab mite Psoroptes ovis. Veterinary Parasitology, 83, 241–252. CrossRefPubMedGoogle Scholar
  12. 12.
    Lefkaditis M.A., Koukeri S.E., Mihalca A.D. 2009. Prevalence and intensity of Otodectes cynotis in kittens from Thessaloniki area, Greece. Veterinary Parasitology, 163, 374–375. CrossRefPubMedGoogle Scholar
  13. 13.
    Li Z.H., Li Z.Q., Qi Y.Y., Wang J. 2015. Diagnosis and therapy of otodectic mange in dogs. Contemporary Animal Husbandry, (6), 18–19Google Scholar
  14. 14.
    Lin Z., Zhuang Y.F., Zhang W.C. 2015. An investigation of parasite infection in pet dogs. Heilongjiang Animal Science and Veterinary Medicine. CrossRefGoogle Scholar
  15. 15.
    Liu G.Q., Liu P.H., Shen L.P., Tang J.Y., Hao J.P. 2000. Diagnose and treatment of otodectic mange in dogs. Chinese Journal of Veterinary Science and Technology, 30, 20.Google Scholar
  16. 16.
    Ljunggren E., Nilsson D., Mattsson J.G. 2003. Expressed sequence tag analysis of Sarcoptes scabiei. Parasitology, 127, 139–145. CrossRefPubMedGoogle Scholar
  17. 17.
    Lohse J., Rinder H., Gothe R., Zahler M. 2002. Validity of species status of the parasitic mite Otodectes cynotis. Medical and Veterinary Entomology, 16, 133–138. CrossRefPubMedGoogle Scholar
  18. 18.
    Six R.H., Clemence R.G., Thomas C.A., Behan S., Boy M.G., Watson P., Benchaoui H.A., Clements P.J.M., Rowan T.G., Jernigan A.D. 2000. Efficacy and safety of selamectin against Sarcoptes scabiei on dogs and Otodectes cynotis on dogs and cats presented as veterinary patients. Veterinary Parasitology, 91, 291–309. CrossRefPubMedGoogle Scholar
  19. 19.
    Sotiraki S.T., Koutinas A.F., Leontides L.S., Adamama-Moraitou K.K., Himonas C.A. 2001. Factors affecting the frequency of ear canal and face infestation by Otodectes cynotis in the cat. Veterinary Parasitology, 96, 309–315. CrossRefPubMedGoogle Scholar
  20. 20.
    Tomisawa H. 1999. A case of mite parasitized in the human external auditory canal. Jibi Inkoka Tokeibu Geka, 71, 276–278Google Scholar

Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2019

Authors and Affiliations

  • Li Hu
    • 1
  • Ya-e Zhao
    • 1
    Email author
  • Dong-ling Niu
    • 1
  • Rui Yang
    • 1
  • Ji-hui Zeng
    • 2
  1. 1.Department of Pathogenic Biology and Immunology, School of Basic Medical SciencesXi’an Jiaotong UniversityXi’anChina
  2. 2.Xi’an PET Animal HospitalXi’anChina

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