Acta Parasitologica

, Volume 64, Issue 2, pp 411–417 | Cite as

Severe Verminous Pneumonia Caused by Natural Mixed Infection with Aelurostrongylus abstrusus and Angiostrongylus chabaudi in a European Wildcat from Western Balkan Area

  • Oliver StevanovićEmail author
  • Anastasia Diakou
  • Simone Morelli
  • Smiljana Paraš
  • Igor Trbojević
  • Drago Nedić
  • Željko Sladojević
  • Dragan Kasagić
  • Angela Di Cesare
Case Report



Aelurostrongylus abstrusus (Railliet, 1898) and Angiostrongylus chabaudi (Biocca, 1957) are important cardiopulmonary metastrongyloids in felids. This case report describes, for the first time, a natural and patent mixed infection caused by A. abstrusus and A. chabaudi in a European wildcat (Felis silvestris silvestris) from Bosnia and Herzegovina. Most important, this is a rare report of fatal parasitism in wildlife, involving a severe verminous bronchopneumonia and gastrointestinal nematode and cestode infection with Toxocara cati, Taenia taeniaeformis, Aonchotheca putorii, and Ancylostoma spp.


Emphasis is set to detailed description of granulomatous, interstitial verminous bronchopneumonia, morphological description of A. abstrusus and A. chabaudi, and molecular confirmation of diagnosis by triplex PCR.


The data provided in this study contribute to the knowledge on the epizootiology and pathological effect of these neglected metastrongyloids in European wildcat.


Angiostrongylus chabaudi Aelurostrongylus abstrusus Wildcat Bosnia and Herzegovina 



We are grateful to Željko Sekulić for the technical support.

Author Contributions

OS performed necropsy, histopathology, and basic parasitological examination; OS, ŽS, DN, IT, and SP drafted manuscript; AD performed detailed morphological and morphometrical examination of nematode samples and revised the manuscript; SM and ADC performed molecular study of samples and revised the manuscript.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no competing interests.


  1. 1.
    Alić A., Traversa D., Duscher G.G., Kadrić M., Di Cesare A., Hodžić A. 2015. Troglostrongylus brevior in an Eurasian lynx (Lynx lynx) from Bosnia and Herzegovina. Parasites & vectors, 8(1), p.653.
  2. 2.
    Biocca E. 1957. Angiostrongylus chabaudi n. sp. parassita del cuore e deivasi polmonari del gatto selvatico (Felis silvestris). Reale Accademia Nazionale dei Lincei, 22, 526–532.Google Scholar
  3. 3.
    Brianti E., Gaglio G., Giannetto S., Annoscia G., Latrofa M.S., Dantas-Torres F., Traversa D., Otranto D. 2012. Troglostrongylus brevior and Troglostrongylus subcrenatus (Strongylida: Crenosomatidae) as agents of broncho-pulmonary infestation in domestic cats. Parasites & vectors, 23, 5:178.
  4. 4.
    Brglez, J., Z. Železnik .1976. Ein Übersicht über die Parasiten der Wildkatze (Felis silvestris Schreber) in Slowenien. Zeitschrift für Jagdwissenschaft. 22, 109–112.Google Scholar
  5. 5.
    Di Cesare A., Di Francesco G., di Regalbono A.F., Eleni C., De Liberato C., Marruchella G., Iorio R., Malatesta D., Romanucci M.R., Bongiovanni L., Cassini R., 2015a. Retrospective study on the occurrence of the feline lungworms Aelurostrongylus abstrusus and Troglostrongylus spp. in endemic areas of Italy. The Veterinary Journal, 203, 233–238.
  6. 6.
    Di Cesare A., Veronesi F., di Regalbono A.F., Iorio R., Traversa D. 2015b. Novel molecular assay for simultaneous identification of neglected lungworms and heartworms affecting cats. Journal of Clinical Microbiology, 53, 3009–3013.
  7. 7.
    Di Cesare A., Laiacona F., Iorio R., Marangi M., Menegotto A. 2016. Aelurostrongylus abstrusus in wild felids of South Africa. Parasitology Research, 115, 3731–5.
  8. 8.
    Diakou A., Di Cesare A., Aeriniotaki T., Traversa D. 2014. First report of Troglostrongylus brevior in a kitten in Greece. Parasitology Research 113, 3895–3898.
  9. 9.
    Diakou A., Psalla D., Migli D., Di Cesare A., Youlatos D., Marcer F., Traversa D. 2016. First evidence of the European wildcat (Felis silvestris silvestris) as definitive host of Angiostrongylus chabaudi. Parasitology Research 115, 1235–1244.
  10. 10.
    Gherman C.M., Ionică A.M., D’Amico G., Otranto D., Mihalca A.D. 2016. Angiostrongylus chabaudi (Biocca, 1957) in wildcat (Felis silvestris silvestris, S) from Romania. Parasitology research, 115, 2511–2517.
  11. 11.
    Giannelli A., Kirkova Z., Abramo F., Latrofa M.S., Campbell B., Zizzo N., Cantacessi C., Dantas-Torres F., Otranto D. 2016. Angiostrongylus chabaudi in felids: new findings and a review of the literature. Veterinary parasitology, 228, 188–192.
  12. 12.
    Jefferies R., Vrhovec M.G., Wallner N., Catalan D.R. 2010. Aelurostrongylus abstrusus and Troglostrongylus sp. (Nematoda: Metastrongyloidea) infections in cats inhabiting Ibiza, Spain. Veterinary Parasitology 173, 344–348.
  13. 13.
    Krone O., Guminsky O., Meinig H., Herrmann M., Trinzen M., Wibbelt G. 2008. Endoparasite spectrum of wild cats (Felis silvestris Schreber, 1777) and domestic cats (Felis catus L.) from the Eifel, Pfalz region and Saarland, Germany. European Journal of Wildlife Research, 54, 95–100.
  14. 14.
    Krüger M., Hertwig S.T., Jetschke G., Fischer M.S. 2009. Evaluation of anatomical characters and the question of hybridization with domestic cats in the wildcat population of Thuringia, Germany. Journal of Zoological Systematics and Evolutionary Research, 47, 268–282.
  15. 15.
    Martinković, F., Sindičić, M., Lučinger, S., Štimac, I., Bujanić, M., Živičnjak, T., Stojčević Jan, D., Šprem, N., Popović, R. and Konjević, D. 2017. Endoparasites of wildcats in Croatia. Veterinarski arhiv, 87, 713–729.Google Scholar
  16. 16.
    Napoli E., Anile S., Arrabito C., Scornavacca D., Mazzamuto M.V., Gaglio G., Otranto D., Giannetto S., Brianti E. 2016. Survey on parasitic infections in wildcat (Felis silvestris silvestris Schreber, 1777) by scat collection. Parasitology Research,
  17. 17.
    Spratt D.M. 2015. Species of Angiostrongylus (Nematoda: Metastrongyloidea) in wildlife: a review. International Journal for Parasitology: Parasites and Wildlife, 4,178–189,
  18. 18.
    Steeb S., Hirzmann J., Eskens U., Volmer K., Bauer C. 2014. Lungenwurm Infektionen bei der Europäischen Wildkatze. Kompakt Vet, 3, 9. (in Deutsch).Google Scholar
  19. 19.
    Stevanović O., Trbojević I., Nikolić S., Santrač V. 2018. The first reported case of advanced aelurostrongylosis in Eurasian badger (Meles meles, L. 1758) in Bosnia and Herzegovina: histopathological and parasitological findings. Parasitology Research, 117, 3029–3032.
  20. 20.
    Traversa D., Lepri E., Veronesi F., Simonato G., Diaferia M., Di Cesare A. 2015. Metastrongyloid infection by Aelurostrongylus abstrusus, Troglostrongylus brevior and Angiostrongylus chabaudi in a domestic cat. International Journal for Parasitology, 45, 685–690.
  21. 21.
    Traversa D., Di Cesare A. 2016. Diagnosis and management of lungworm infections in cats: cornerstones, dilemmas and new avenues. Journal of feline medicine and surgery, 18, 7–20.
  22. 22.
    Varcasia A., Tamponi C., Brianti E., Cabras P.A., Boi R., Pipia A.P., Giannelli A., Otranto D., Scala A. 2014. Angiostrongylus chabaudi Biocca, 1957: a new parasite for domestic cats? Parasites & vectors, 17, 7–588.
  23. 23.
    Veronesi F., Traversa D., Lepri E., Morganti G., Vercillo F., Grelli D., Cassini R., Marangi M., Iorio R., Ragni B., Di Cesare A. 2016. Occurrence of lungworms in european wildcats (Felis silvestris silvestris) of central Italy. Journal of Wildlife Diseases, 52, 270–278.

Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2019

Authors and Affiliations

  • Oliver Stevanović
    • 1
    Email author
  • Anastasia Diakou
    • 2
  • Simone Morelli
    • 3
  • Smiljana Paraš
    • 4
  • Igor Trbojević
    • 4
  • Drago Nedić
    • 5
  • Željko Sladojević
    • 5
  • Dragan Kasagić
    • 5
  • Angela Di Cesare
    • 3
  1. 1.Veterinary Ambulance “BL vet” Banja LukaBanja LukaBosnia and Herzegovina
  2. 2.Laboratory of Parasitology and Parasitic Diseases, School of Veterinary Medicine, Faculty of Health SciencesAristotle University of ThessalonikiThessalonikiGreece
  3. 3.Faculty of Veterinary MedicineUniversity of TeramoTeramoItaly
  4. 4.Faculty of SciencesUniversity of Banja LukaBanja LukaBosnia and Herzegovina
  5. 5.Public Veterinary Institute of Republic of Srpska „Dr. Vaso Butozan“Banja LukaBosnia and Herzegovina

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