Advertisement

Acta Parasitologica

, Volume 64, Issue 2, pp 228–231 | Cite as

Molecular Characterization of Entamoeba spp. in Wild Taihangshan Macaques (Macaca mulatta tcheliensis) in China

  • Qingxun Zhang
  • Kongshang Liu
  • Chengmin Wang
  • Jing Luo
  • Jiqi LuEmail author
  • Hongxuan HeEmail author
Short Communication
  • 58 Downloads

Abstract

Background

Entamoeba spp. is one of the most common enteric parasites of humans and animals.

Purpose

However, little information is available regarding prevalence and genotypes of Entamoeba spp. in wild Taihangshan macaques (Macaca mulatta tcheliensis).

Methods

In the present study, a total of 458 fecal samples from wild rhesus macaque in Taihangshan mountains area between September 2015 and November 2016, were collected and examined for the presence of six Entamoeba species by PCR amplification of the SSU rRNA gene.

Results

The overall prevalence of Entamoeba spp. infection was 89.96% (412/458). Four species of Entamoeba detected in our survey were E. chattoni (88.43%), E. hartmanni (79.91%), E. coil (69.87%) and E. dispar (58.30%), and among these, 398 (84.93%) were mixed infections. Phylogenetic analysis revealed that isolates were clustered into four known genotypes.

Conclusion

The present study firstly provides important information about the prevalence and diversity of Entamoeba species infecting wild Taihangshan macaques in China. Enough attention should be paid to monitor the potential interspecies transmission in the region.

Keywords

China Entamoeba spp. Genotypes Prevalence Taihangshan macaques 

Notes

References

  1. 1.
    Debenham J.J., Tysnes K., Khunger S., Robertson L.J. 2017. Occurrence of Giardia, Cryptosporidium, and Entamoeba in wild rhesus macaques (Macaca mulatta) living in urban and semi-rural North-West India. International Journal for Parasitology: Parasites and Wildlife, 6, 29–34PubMedGoogle Scholar
  2. 2.
    Dong H., Li J., Qi M., Wang R., Yu F., Jian F., et al. 2017. Prevalence, molecular epidemiology, and zoonotic potential of Entamoeba spp. in nonhuman primates in China. Infection, Genetics and Evolution, 54, 216–220CrossRefPubMedGoogle Scholar
  3. 3.
    Feng M., Cai J., Min X., Fu Y., Xu Q., Tachibana H., et al. 2013. Prevalence and genetic diversity of Entamoeba species infecting macaques in southwest China. Parasitology Research, 112, 1529–1536CrossRefPubMedGoogle Scholar
  4. 4.
    Feng M., Yang B., Yang L., Fu Y., Zhuang Y., Liang L., et al. 2011. High prevalence of Entamoeba infections in captive long-tailed macaques in China. Parasitology Research, 109, 1093–1097CrossRefPubMedGoogle Scholar
  5. 5.
    Gillespie T.R., Greiner E.C., Chapman C.A. 2005. Gastrointestinal parasites of the colobus monkeys of Uganda. Journal of Parasitology, 91, 569–573CrossRefPubMedGoogle Scholar
  6. 6.
    Gomes T.D.S., Garcia M.C., Souza C.F.D., Werneck D.M.H., Peralta J.M., Peralta R.H.S. 2014. Differential diagnosis of Entamoeba spp. in clinical stool samples using SYBR green real-time polymerase chain reaction. Scientific World Journal, 2014: 645084Google Scholar
  7. 7.
    Guan Y., Feng M., Cai J., Min X., Zhou X., Xu Q., et al. 2016. Comparative analysis of genotypic diversity in Entamoeba nuttalli isolates from Tibetan macaques and rhesus macaques in China. Infection, Genetics and Evolution, 38, 126–131CrossRefPubMedGoogle Scholar
  8. 8.
    Hamzah Z., Petmitr S., Mungthin M., Leelayoova S., Chavalitshewinkoon-Petmitr P. 2006. Differential detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii by a single-round PCR assay. Journal of Clinical Microbiology, 44, 3196–3200CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Levecke B., Dreesen L., Dorny P., Verweij J.J., Vercammen F., Casaert S., et al. 2010. Molecular identification of Entamoeba spp. in captive nonhuman primates. Journal of Clinical Microbiology, 48, 2988–2990CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Levecke B., Dorny P., Vercammen F., Visser L.G., Van Esbroeck M., Vercruysse J., et al. 2015. Transmission of Entamoeba nuttalli and Trichuris trichiura from Nonhuman Primates to Humans. Emerging Infectious Diseases, 21, 1871CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Li D.Y., Xu H.L., Smith D.G., Cheng A.C., Trask J.S., Zhu Q., et al. 2011. Phylogenetic analysis of Chinese rhesus macaques (Macaca mulatta) based on mitochondrial control region sequences. American Journal of Primatology, 73, 883–895CrossRefPubMedGoogle Scholar
  12. 12.
    Li J., Dong H., Wang R., Yu F., Wu Y., Chang Y., et al. 2017. An investigation of parasitic infections and review of molecular characterization of the intestinal protozoa in nonhuman primates in China from 2009 to 2015. International Journal for Parasitology: Parasites and Wildlife, 6, 8–15PubMedGoogle Scholar
  13. 13.
    Lu J.Q., Hou J.H., Wang H.F., Qu W.Y. 2007. Current status of Macaca mulatta in Taihangshan mountains area, Jiyuan, Henan, China. International Journal of Primatology, 28, 1085–1091CrossRefGoogle Scholar
  14. 14.
    Pourrut X., Diffo J.L.D., Somo R.M., Bilong C.B., Delaporte E., LeBreton M., et al. 2011. Prevalence of gastrointestinal parasites in primate bushmeat and pets in Cameroon. Veterinary Parasitology, 175, 187–191CrossRefPubMedGoogle Scholar
  15. 15.
    Ragazzo L.J., Zohdy S., Velonabison M., Herrera J., Wright P.C., Gillespie T.R. 2018. Entamoeba histolytica infection in wild lemurs associated with proximity to humans. Veterinary Parasitology, 249, 98–101CrossRefPubMedGoogle Scholar
  16. 16.
    Ravdin, J.I., 1989. Entamoeba histolytica: from adherence to enteropathy. Journal of Infectious Diseases, 159, 420–429CrossRefPubMedGoogle Scholar
  17. 17.
    Tachibana H., Cheng X.J., Kobayashi S., Matsubayashi N., Gotoh S., Matsubayashi K. 2001. High prevalence of infection with Entamoeba dispar, but not E. histolytica, in captive macaques. Parasitology Research, 87, 14–17CrossRefPubMedGoogle Scholar
  18. 18.
    Tachibana H., Yanagi T., Akatsuka A., Kobayashi S., Kanbara H., Tsutsumi V. 2009. Isolation and characterization of a potentially virulent species Entamoeba nuttalli from captive Japanese macaques. Parasitology, 136, 1169–1177CrossRefPubMedGoogle Scholar
  19. 19.
    Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729CrossRefPubMedPubMedCentralGoogle Scholar
  20. 20.
    Tanyuksel M., Petri W.A. 2003. Laboratory diagnosis of amebiasis. Clinical microbiology reviews, 16, 713–729CrossRefPubMedPubMedCentralGoogle Scholar
  21. 21.
    Verweij J.J., Polderman A.M., Clark C.G. 2001. Genetic variation among human isolates of uninucleated cyst-producing Entamoeba species. Journal of Clinical Microbiology, 39, 1644–1646.CrossRefPubMedPubMedCentralGoogle Scholar
  22. 22.
    Zhang Y. P., Shi L. M. 1993. Phylogeny of rhesus monkeys (Macaca mulatta) as revealed by mitochondrial DNA restriction enzyme analysis. International Journal of Primatology, 14, 587–605CrossRefGoogle Scholar

Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2019

Authors and Affiliations

  1. 1.National Research Center for Wildlife-Borne Diseases, Institute of ZoologyChinese Academy of SciencesBeijingChina
  2. 2.University of the Chinese Academy of SciencesBeijingChina
  3. 3.Institute of Biodiversity and EcologyZhengzhou UniversityZhengzhouChina

Personalised recommendations