Acta Parasitologica

, Volume 64, Issue 1, pp 148–151 | Cite as

Detection of Antibodies Against Toxoplasma gondii in Indian Cattle by Recombinant SAG2 Enzyme-Linked Immunosorbent Assay

  • Vikrant SudanEmail author
  • A. K. Tewari
  • Harkirat Singh



A large number of recombinant proteins are tested over a period of time for diagnosis of toxoplasmosis throughout the world. However, such literature is very much limited from an Indian perspective.


The aim of the study was to assess the sero prevalence of Toxoplasma gondii in adult cattle from Kerala, India, using recombinant surface antigen 2 (recSAG2) protein.


An antibody detection recombinant ELISA specific for T gondii was laboratory-standardized using recSAG2 protein. The optimum antigen concentration, serum concentration, and conjugate dilutions were determined by the initial checkerboard titrations. Subsequently, the diagnostic potential of the recombinant protein was assessed with 258 field sera samples cattle and compared with indirect fluorescent antibody test (IFAT).


Among the cattle sera tested, 61.5% showed sero positivity of T. gondii-speciic IgG. When compared to IFAT, the sensitivity of the recSAG2 ELISA was found to be 80.00% with 88.57% specificity at 95% confidence interval with substantial agreement between the tests.


The results of present study have revealed the presence of high seroprevalence of the parasite and, hence, immense public health significance.


Cattle IFAT India rec-SAG2-ELISA Toxoplasma gondii 



The authors acknowledge Dr J P Dubey, USDA, Beltsville, USA, for providing the reference sera. The authors are also thankful to the Director, Indian Veterinary Research Institute for providing the facilities as well as to the ICAR for the fellowship awarded to the first author during the perusal of his master’s programme.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


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Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2019

Authors and Affiliations

  • Vikrant Sudan
    • 1
    • 2
    Email author
  • A. K. Tewari
    • 1
    • 3
  • Harkirat Singh
    • 1
    • 4
  1. 1.Indian Veterinary Research Institute (IVRI)BareillyIndia
  2. 2.Department of Parasitology, College of Veterinary Sciences and Animal HusbandryUP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU)MathuraIndia
  3. 3.Division of ParasitologyIndian Veterinary Research Institute (IVRI)BareillyIndia
  4. 4.Department of Veterinary Parasitology, College of Veterinary ScienceGADVASULudhianaIndia

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