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Acta Parasitologica

, Volume 64, Issue 1, pp 129–137 | Cite as

Molecular Genetic Studies on Myxobolus cylindricus and Henneguya mystasi (Myxosporea: Myxobolidae) Infecting Two Indian Fish Species, Channa gachua and Mystus vittatus, Respectively

  • A. Chaudhary
  • A. Gupta
  • U. Goswami
  • G. Cech
  • K. Molnár
  • H. S. Singh
  • C. SzékelyEmail author
Original Paper
  • 22 Downloads

Abstract

Introduction

Myxozoan infections of Indian freshwater fishes are relatively well studied, but their validity is supported with molecular and phylogenetic data only for a few species.

Objectives

The primary objective was to present molecular data for two Myxozoan species, Myxobolus cylindricus and Henneguya gachua collected from Indian freshwater fishes, the dwarf snakehead Channa gachua and the striped dwarf catfish Mystus vittatus, respectively.

Methods

Various organs of 56 C. gachua and 48 M. vittatus were dissected. Myxozoan plasmodia with mature spores were collected from the gills under a dissecting microscope. Spores obtained from plasmodia from infected hosts were fixed in 80% ethanol in vials and sent for further morphological and molecular examinations to Hungary. The 18S rDNA gene of Myxobolus and Henneguya spp. was amplified using nested polymerase chain reaction (PCR). Phylogenetic analysis was performed using maximum likelihood (ML) and Bayesian inference (BI) methods.

Results

Morphological characteristics of M. cylindricus and H. mystasi spores corresponded to the original descriptions made by Sarkar, Mazumdar and Pramanik, 1985 and Haldar, Samal, and Mukhopadhyay, 1997, respectively. Phylogenetic analysis of the 18S rDNA gene revealed that the sequences of M. cylindricus differed from those of most Indian Myxobolus sp., known mostly from cyprinid fishes and formed a subgroup with Myxobolus neurophilus, a parasite of a perciform host, and with Henneguya chaudhuryi, a species belonging to a different genus but described from a closely related channid host. It was also closely related to another Henneguya species, H. lesteri, described from Sillago analis, a coastal fish. Henneguya mystasi had the closest similarity to Henneguya bicaudi, a species described from an Indian cyprinid fish and to Henneguya pellucida reported from a characid fish known from South America.

Conclusion

Molecular data received by us gives a solid basis for further identification of these myxozoans, the pathogenicity of which probably plays an economic role at culturing the hosts.

Keywords

Myxobolus Henneguya Morphology Molecular study 18S rDNA Meerut India 

Notes

Acknowledgements

The authors would like to thank the Head of the Department of Zoology, Chaudhary Charan Singh University, Meerut, Uttar Pradesh, India, for providing laboratory facilities. The work was supported by a grant from the Uttar Pradesh Government, Centre of Excellence, India, to HSS (Project number no. 1486/70-4-2011-46(43)/2010) and by the European Regional and Development Fund and the Government of Hungary within the Project GINOP-2.3.2-15-2016-00025.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. 1.
    Abraham, T.J., Banerjee, S., Patra, A., Sarkar, A., Adikesavalu, H., Dash, G. 2015. Molecular phylogeny of Myxobolus orissae (Myxosporea: Myxobolidae) infecting the gill lamellae of mrigal carp Cirrhinus mrigala (Actinopterygii: Cyprinidae). Molecular Biology Research Communications, 4, 15–24.Google Scholar
  2. 2.
    Andree, K.B., Székely, C., Molnár, K., Gresoviac, S.J., Hedrick, R.P. 1999. Relationships among members of the genus Myxobolus (Myxozoa : Bilvalvidae) based on small subunit ribosomal DNA sequences. Journal of Parasitology, 85, 68–74.Google Scholar
  3. 3.
    Barta, J.R., Martin, D.S., Libetator, P.A., Dashkevicz, M., Anderson, J.W., Feighner, S.D., et al., 1997. Phylogenetic relationships among eight Eimeria species infecting domestic fowl inferred using complete small subunit ribosomal DNA sequences. Journal of Parasitology, 83, 262–271.Google Scholar
  4. 4.
    Basu, S., Modak, B.K., Haldar, D.P. 2009. Two new species of Myxobolus Bütschli, 1882 (Myxozoa: Mysporea: Bivalvulida) from food fishes of West Bengal, India-a light and scanning electron microscopy study. Acta Protozoologica, 48, 89.Google Scholar
  5. 5.
    Bhatt, V.S., Siddiqui, W.A. 1964. Four new species of myxosporidia from the Indian freshwater fish, Ophiocephalus punctatus Bloch. Journal of Protozoology, 11, 314–316.  https://doi.org/10.1111/j.1550-7408.1964.tb01761.x.
  6. 6.
    Cech, G., Molnár, K., Székely, C. 2012. Molecular genetic studies on morphologically indistinguishable Myxobolus spp. infecting cyprinid fishes, with the description of three new species, M. alvarezae sp. nov., M. sitjae sp. nov. and M. eirasianus sp. nov. Acta Parasitologica, 57, 354–366. DOI: 0.2478/s11686-012-0045-2.Google Scholar
  7. 7.
    Chaudhary, A., Molnár, K., Gupta, A., Cech, G., Singh, H.S., Székely, C. 2017. Redescription of Henneguya chaudhuryi (Bajpai & Haldar, 1982) (Myxosporea: Myxobolidae), infecting the gills of the freshwater fish Channa punctata (Bloch) (Perciformes: Channidae) in India. Systematic Parasitology, 94, 403–411.  https://doi.org/10.1007/s11230-017-9705-9.
  8. 8.
    Chaudhary, A., Goswami, U., Gupta, A., Cech, G., Singh, H.S., Molnár, K., Székely, C., Sharma, B. 2018. Morphological, histological, and molecular description of Myxobolus ompok n. sp. (Myxosporea: Myxobolidae), a kidney myxozoan from Pabdah catfish Ompok pabda (Hamilton, 1822) (Siluriformes: Siluridae) in India. Parasitology Research, 117, 1899–1905.  https://doi.org/10.1007/s00436-018-5882-y.
  9. 9.
    Dar, S.A., Kaur, H., Chishti, M.Z. 2017. Myxobolus chushi n. sp (Myxozoa: Myxosporea) parasitizing Schizothorax niger (Heckel), a native cyprinid fish from Wullar Lake in Kashmir Himalayas. Parasitology International, 66, 272–278.  https://doi.org/10.1016/j.parint.2017.02.004.
  10. 10.
    Dyková, I., Lom J. 2007. Histopathology of protistan and myxozoan infections in fishes. An Atlas. Academia, Praha, pp. 219.Google Scholar
  11. 11.
    Eiras, J.C. 2002. Synopsis of the species of the genus Henneguya Thélohan, 1892 (Myxozoa: Myxosporea: Myxobolidae). Systematic Parasitology, 52, 43–54.  https://doi.org/10.1023/a:1015016312195.
  12. 12.
    Eiras, J.C., Adriano, E.A. 2012. A checklist of new species of Henneguya Thélohan, 1892 (Myxozoa: Myxosporea, Myxobolidae) described between 2002 and 2012. Systematic Parasitology, 83, 95–104.  https://doi.org/10.1007/s11230-012-9374-7.
  13. 13.
    Eiras, J.C., Molnár, K., Lu, Y.S. 2005. Synopsis of the species of Myxobolus Bütschli, 1882 (Myxozoa: Myxosporea: Myxobolidae). Systematic Parasitology, 61, 1–46.  https://doi.org/10.1007/s11230-004-6343-9.
  14. 14.
    Eiras, J.C., Zhang, J.Y., Molnár, K. 2014. Synopsis of the species Myxobolus Bütschli, 1882 (Myxozoa: Myxosporea) described between 2005 and 2013. Systematic Parasitology, 88, 11–36.  https://doi.org/10.1007/s11230-014-9484-5.
  15. 15.
    Eszterbauer, E. 2004. Genetic relationship among gill-infecting Myxobolus species (Myxosporea) of cyprinids: molecular evidence of importance of tissue-specificity. Diseases of Aquatic Organisms, 58, 35–40.  https://doi.org/10.3354/dao058035.
  16. 16.
    Eszterbauer, E., Székely, C. 2004. Molecular phylogeny of the kidney parasitic Sphaerospora renicola from common carp (Cyprinus carpio) and Sphaerospora sp. from goldfish (Carassius auratus auratus). Acta Veterinaria Hungarica, 52, 469–478.  https://doi.org/10.1556/avet.52.2004.4.9.
  17. 17.
    Feist, S.W., Longshaw, M. 2006. Phylum Myxozoa. In: (Ed P.T.K. Woo) Fish diseases and disorders: Protozoan and metazoan infections, 2nd ed. CABI Publishing, UK, pp. 230–296.Google Scholar
  18. 18.
    Ghosh, S., Bandyopadhyay, P.K. 2017. Morphotaxonomical description of three new species of Myxobolus Bütschli, 1882 (Myxozoa: Myxosporea: Bivalvulida) from edible freshwater fishes of West Bengal, India. Journal of Parasitic Diseases, 41, 97–105.  https://doi.org/10.1007/s12639-016-0757-6.
  19. 19.
    Gupta, A., Kaur, H. 2017a. Morphological and molecular characterization of Myxobolus puntiusii n. sp (Cnidaria: Myxosporea) infecting Puntius sophore Hamilton, 1822 from Ranjit Sagar Wetland, Punjab (India). Turkish Journal of Zoology, 41, 791–799.  https://doi.org/10.3906/zoo-1606-47.
  20. 20.
    Gupta, A., Kaur, H. 2017b. A new pathogen, Myxobolus holzerae (Myxosporea: Myxozoa) causing severe gill disease in an Indian major carp Labeo rohita in a cold water wetland, Punjab (India). Microbial Pathogenesis, 111, 244–251.  https://doi.org/10.1016/j.micpath.2017.08.044.
  21. 21.
    Gupta, A., Kaur, H. 2018. Myxobolus okamurae sp. Nov. (Myxosporea: Myxozoa) causing severe gill myxoboliosis in the cyprinid Labeo bata in a cold water wetland, Punjab (India). Microbial Pathogenesis, 115, 86–92.  https://doi.org/10.1016/j.micpath.2017.12.041.
  22. 22.
    Gupta, S., Khera, S. 1988. On one new and one already known species of the genus Myxobolus from fresh water fishes of India. Research Bulletin (Science), Punjab University, 39 (III-IV), 173–179.Google Scholar
  23. 23.
    Haldar, D.P., Mukherjee, M. 1985. Studies on Bivalvulida (Myxozoa: Myxosporea): Observations on two new species of Henneguya from food fishes of West Bengal, India. Archiv für Protistenkunde, 130, 419–425.  https://doi.org/10.1016/s0003-9365(85)80053-1.
  24. 24.
    Haldar, D.P., Samal, K.K., Mukhopadhyay, D. 1997. Studies in the protozoan parasites of fishes in Orissa: five new species of the genera Henneguya, Thelohanellus and Unicauda (Myxozoa: Bivalvulida). Journal of the Bengal Natural History Society, 16, 50–63.Google Scholar
  25. 25.
    Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Research, 41, 95–98.Google Scholar
  26. 26.
    Hallett, S.L., Diamant, A. 2001. Ultrastructure and small subunit ribosomal DNA sequence of Henneguya lesteri n. sp. (Myxosporea), a parasite of sand whiting Sillago analis (Sillaginidae) from the coast of Queensland, Australia. Diseases of Aquatic Organisms, 46, 197–212.  https://doi.org/10.3354/dao046197.
  27. 27.
    Kalavati, C., Nandi, N.C. 2007. Handbook on myxosporean parasites of Indian fishes. New Delhi: Zoological Survey of India, Kolkata, India, pp. 294.Google Scholar
  28. 28.
    Kalavati, C., Sandeep, B.V., Narasimhamurti, C.C. 1981. Two new species of myxosporidians, Myxosoma channai n. sp., and Myxobolus tripathii n. sp., from freshwater fishes of Andhra Pradesh. Proceedings of the Indian Academy of Sciences, 90, 61–78.Google Scholar
  29. 29.
    Kaur, H., Attri, R. 2015. Morphological and molecular characterization of Henneguya bicaudi n. sp. (Myxosporea: Myxobolidae) infecting gills of Cirrhinus mrigala (Ham.) in Harike Wetland, Punjab (India). Parasitology Research, 114, 4161–4167.  https://doi.org/10.1007/s00436-015-4647-0.
  30. 30.
    Kaur, H., Singh, R. 2008. Observations on one new species, of genus Myxobolus - M. naini and redescription of M. magauddi recorded from freshwater fishes of Kanjali Wetland of Punjab, India. Proceedings of the 20 th National Congress of Parasitology, pp. 75–79.Google Scholar
  31. 31.
    Kaur, H., Singh, R. 2012. Two new species of Myxobolus (Myxozoa: Myxosporea: Bivalvulida) from freshwater fishes of Punjab wetlands (India). Journal of Parasitic Diseases, 35, 33–41.  https://doi.org/10.1007/s12639-011-0024-9.
  32. 32.
    Lalitha Kumari, P.S. 1969. Studies on parasitic protozoa (Myxosporidia) of fresh water fishes of Andhra Pradesh, India. Rivista di Parassitologia, 30, 153–226.Google Scholar
  33. 33.
    Landsberg, J.H., Lom, J. 1991. Taxonomy of the genera of the Myxobolus/Myxosomagroup (Myxobolidae: Myxosporea), current listing of species and revision of synonyms. Systematic Parasitology, 18, 165–186.Google Scholar
  34. 34.
    Lom, J., Arthur, J.R. 1989. A guideline for preparation of species description in Myxosporea. Journal of Fish Diseases, 12, 151–156.  https://doi.org/10.1111/j.1365-2761.1989.tb00287.x.
  35. 35.
    Lom, J., Dyková, I. 1992. Fine-structure of triactinomyxon early stages and sporogony-myxosporean and actinosporean features compared. Journal of Protozoology,. 39, 16–27.  https://doi.org/10.1111/j.1550-7408.1992.tb01279.x.
  36. 36.
    Lom, J., Dyková, I. 2006. Myxozoan genera: definition and notes on taxonomy, life-cycle terminology and pathogenic species. Folia Parasitologica, 53, 1–36.Google Scholar
  37. 37.
    Milne, I., Lindner, D., Bayer, M., Husmeier, D., McGuire, G., Marshall, D.F., Wright, F. 2008. TOPALi v2: as rich graphical interface for evolutionary analyses of multiple alignments on HPC clusters and multicore desktops. Bioinformatics, 25, 126–127.  https://doi.org/10.1093/bioinformatics/btn575.
  38. 38.
    Molnár, K. 2002. Site preference of myxosporeans in the gill. Diseases of Aquatic Organisms, 48, 197–207.  https://doi.org/10.3354/dao048197.
  39. 39.
    Molnár, K. 2007. Site preference of myxozoans in the kidneys of Hungarian fishes. Diseases of Aquatic Organisms, 78, 45–53.  https://doi.org/10.3354/dao01827.
  40. 40.
    Molnár, K., Cech, G., Székely, C. 2011. Histological and molecular studies of species of Myxobolus Bütschli, 1882 (Myxozoa: Myxosporea) in the gills of Abramis, Blicca and Vimba spp. (Cyprinidae), with the redescription of M. macrocapsularis Reuss, 1906 and M. bliccae Donec & Tozyyakova, 1984. Systematic Parasitology, 79, 109–121.  https://doi.org/10.1007/s11230-011-9292-0.
  41. 41.
    Molnár, K., Eszterbauer, E. 2015. Specificity of Infection Sites in Vertebrate Hosts. In: (Eds B. Okamura, A. Gruhl, J.L. Bartholomew) Myxozoan Evolution, Ecology and Development. Springer International Publishing, Cham, Switzerland, pp. 295–313.Google Scholar
  42. 42.
    Molnár, K., Eszterbauer, E., Székely, C., Dán, Á, Harrach, B. 2002. Morphological and molecular biological studies on intramuscular Myxobolus spp. of cyprinid fish. Journal of Fish Diseases, 25, 643–652. DOI:/ https://doi.org/10.1046/j.1365-2761.2002.00409.x.
  43. 43.
    Rajesh, S.C., Banerjee, S., Patra, A., Dash, G., Abraham, T.H. 2014. Molecular characterization if Myxobolus cuttacki (Myxozoa: Myxosporea, Bivalvulida) infecting gill lamellae of minor carp Labeo bata (Ham.). Molecular Biology Research Communications, 3, 231–234.Google Scholar
  44. 44.
    Sarkar, N.K. 1982. On three new myxosporidian parasites (Myxozoa) of the Ophiocephalid fishes of West Bengal, India. Acta Protozoologica, 21, 239–244.Google Scholar
  45. 45.
    Sarkar, N.K. 1985. Myxosporidian Henneguya mystusii sp. n. (Myxozoa: Myxosporea) from the gill of a fresh water teleost fish Mystus sp. Acta Protozoologica, 24, 56–58.Google Scholar
  46. 46.
    Sarkar, N.K., Mazumdar, S.K., Pramanik, A. 1985. Observations on four new species of Myxosporidia (Myxozoa) from channid (Ophiocephalid) fishes of West Bengal, India. Archiv für Protistenkunde,130, 289–296.Google Scholar
  47. 47.
    Székely, C., Cech, G., Chaudhary, A., Borzák, R., Singh, H.S., Molnár, K. 2015. Myxozoan infections of the three Indian major carps in fish ponds around Meerut, UP, India, with descriptions of three new species, Myxobolusbasuhaldari sp. n., M. kalavatiae sp. n. and M. meerutensis sp. n., and the redescription of M. catlaeand M. bhadrensis. Parasitology Research, 114, 1301–1311..  https://doi.org/10.1007/s00436-014-4307-9.
  48. 48.
    Tamura, K., Stecher, G., Peterson, D., Filipski, A., Kumar, S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution, 30, 2725–2729.  https://doi.org/10.1093/molbev/mst197.
  49. 49.
    Thompson, J.D., Higgins, D.G., Gibson, T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673–4680.Google Scholar

Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2019

Authors and Affiliations

  • A. Chaudhary
    • 1
  • A. Gupta
    • 2
  • U. Goswami
    • 1
    • 3
  • G. Cech
    • 3
  • K. Molnár
    • 3
  • H. S. Singh
    • 1
  • C. Székely
    • 3
    Email author
  1. 1.Department of ZoologyChaudhary Charan Singh UniversityMeerutIndia
  2. 2.Department of ZoologyD.N.P.G. CollegeMeerutIndia
  3. 3.Institute for Veterinary Medical Research, Centre for Agricultural ResearchHungarian Academy of SciencesBudapestHungary

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