Diaphorocleidus machacae n. sp. (Monogenea) Infecting the Gill Lamellae of Brycon guatemalensis (Characiformes: Bryconidae) from the Rio Lacantún Basin in Chiapas, Mexico



There are currently nine known gill monogenean species of Diaphorocleidus Jogunoori et al. (Systematic Parasitology 58:115–124, 2004) (Dactylogyridae) which are dispersed within and/or among five fish families in the Neotropical Characiformes: Bryconidae, Acestrorhynchidae, Characidae, Iguanodectidae, and Hemiodontidae. During a fish parasitological study carried out in the Rio Lacantún from Chiapas, Mexico, a new species of Diaphorocleidus on the gill lamellae of Brycon guatemalensis Regan, 1908, was discovered.


Our objective was to morphologically describe the new parasite species by comparing it with previously described species of Diaphorocleidus and other known dactylogyrids on Characiformes.


Worms were fixed with 4–5% formalin solution, observed and measured as temporary or permanent mounts stained with Gomori’s trichrome, and mounted in Canada balsam. In addition, some specimens were mounted on slides using a mixture of lactic acid (LA) and glycerin-ammonium picrate (GAP) and then remounted in Canada balsam to obtain measurements of the haptoral structures and copulatory complex. Drawings were made with the aid of a drawing tube using a Leica microscope DM2500 with Nomarski interference contrast.


The new species differs from previously described congeneric species in having comparatively biggest body size [i.e., 800–1200 µm vs. 222–340 µm in Diaphorocleidus armillatus Jogunoori et al. (Systematic Parasitology 58:115–124, 2004) (type species of the genus)]; two prostatic reservoirs; rod-shaped accessory piece; a sucker-like structure associated with the genital pore; vaginal duct looping left intestinal cecum and opening on the dextro-dorsal margin of body; anchors with conspicuous basal folds on the deep roots weakly developed.


Consistently with the previous phylogenetic hypotheses about the origin of bryconids (20 mya late Oligocene–early Miocene) in Central America, that morphological differentiation in D. machacae n. sp. in Mexico may somewhat reflect the insights of an early dispersal of a common ancestor of Diaphorocleidus to the area in comparison with that of a late invasion of their congeners [i.e., according to the origin of B. petrosus (10 mya) 10 mya] in Central America. The present finding brings to ten, the number of known species of Diaphorocleidus, and represents the second described species of this genus occurring in a freshwater native host in Mexico.

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  1. 1.

    Abe K T, Mariguela T C, Avelino G S, Foresti F, Oliveira C 2014 Systematic and historical biogeography of the Bryconidae (Ostariophysi: Characiformes) suggesting a new rearrangement of its genera and an old origin of Mesoamerican ichthyofauna Evolutioanry Biology, 14, 152

    Google Scholar 

  2. 2.

    Agarwal N, Kritsky D C 1998 Neotropical Monogenoidea 33 Three new species of Ancistrohaptor n. g. (Dactylogyridae, Ancyrocephalinae) on Triportheus spp. (Teleostei, Characidae) from Brazil, with checklist of ancyrocephalines recorded from neotropical characiform fishes Systematic Parasitology, 39, 59–69

    Article  Google Scholar 

  3. 3.

    Braga M P, Razzolini E, Boeger W A 2015 Drivers of parasite sharing among Neotropical freshwater fishes Journal of Animal Ecology, 84, 487–497

    Article  Google Scholar 

  4. 4.

    Braga M P, Sabrina B L, Boeger W A 2014 Patterns of interaction between Neotropical freshwater fishes and their gill Monogenoidea (Platyhelminthes) Parasitology Research, 113, 481–490 https://doi.org/10.1007/s00436-013-3677-8

    Article  PubMed  Google Scholar 

  5. 5.

    Cuglianna A M, da Silva Cordeiro N, Luque J L 2003 Annulotrematoides bryconi sp. n. (Monogenea: Dactylogyridae) parasitic on Brycon cephalus (Osteichthyes: Characidae) from Brazil Folia Parasitologica, 50, 272–274

    Article  Google Scholar 

  6. 6.

    Jogunoori W, Kritsky D C, Venkatanarasaiah J 2004 Neotropical Monogenoidea. 46. Three new species from the gills of introduced aquarium fishes in India, the proposal of Heterotylus n. g. and Diaphorocleidus n g, and the reassignment of some previously described species of Urocleidoides Mizelle & Price, 1964 (Polyonchoinea: Dactylogyridae) Systematic Parasitology, 58, 115–124

    CAS  Article  Google Scholar 

  7. 7.

    Kritsky D C, Boeger W A, Jégu M 1996 Neotropical Monogenoidea. 28. Ancyrocephalinae (Dactylogyridae) of piranha and their relatives (Teleostei, Serrasalmidae) from Brazil and French Guiana: species of Notozothecium Boeger and Kritsky, 1988, and Mymarothecium gen n Journal Helminthological Society of Washington, 63, 153–175

    Google Scholar 

  8. 8.

    Kritsky D C, Boeger W A, Thatcher V E 1985 Neotropical Monogenea. 7. Parasites of the pirarucu Arapaima gigas (Cuvier), with descriptions of two new species and redescription of Dawestrema cycloancistrium Price and Nowlin, 1967 (Dactylogyridae: Ancyrocephalinae) Proceedings of the Biological Society of Washington, 98, 321–331

    Google Scholar 

  9. 9.

    Kritsky DC, Boeger WA, Van Every LR 1992 Neotropical Monogenoidea 17 Anacanthorus Mizelle and Price, 1965 (Dactylogyridae, Anacanthorinae) from characoid fishes of the Central Amazon Journal of the Helminthological Society of Washington, 59, 25–51

    Google Scholar 

  10. 10.

    Kritsky D C, Thatcher, V E, Kayton R J 1979 Neotropical Monogenoidea 2 The Anacanthorinae Price, 1967, with the proposal of four new species of Anacanthorus Mizelle & Price, 1965, from Amazonian fishes Acta Amazonica, 9, 355–361

    Article  Google Scholar 

  11. 11.

    Kritsky D C, Thatcher V E, Kayton R J 1980 Neotropical Monogenoidea 3 Five new species from South America with the proposal of Tereancistrium gen n and Trinibaculum gen n (Dactylogyridae: Ancyrocephalinae) Acta Amazonica 10, 2, 411–417

    Article  Google Scholar 

  12. 12.

    Leão M SL, São C S C , Cohen S C 2015 Anacanthorus toledoensis n. sp. and Mymarothecium ianwhittingtoni n. sp. (Dactylogyridae: Monogenoidea) parasitizing cage-reared Piaractus mesopotamicus (Characiformes, Characidae) in the State of Paraná, Brazil. Compar Parasitol, 82, 269–274

    Article  Google Scholar 

  13. 13.

    Leigh E G, O´Dea A, Vermeij G J 2014 Historical biogeography of the Isthmus of Panama Biological Reviews, 148–72 https://doi.org/10.1111/brv12048

    Article  PubMed  Google Scholar 

  14. 14.

    Mendoza-Franco E F, Aguirre-Macedo M L, Vidal-Martínez V M 2007 New and previously described species of Dactylogyridae (Monogenoidea) from the gills of Panamanian freshwater fishes (Teleostei) Journal of Parasitology, 93, 761–771

    Article  Google Scholar 

  15. 15.

    Mendoza-Franco E F, Caspeta-Mandujano J M, Salgado-Maldonado G 2013 New species of Cacatuocotyle (Monogenoidea, Dactylogyridae) parasitising the anus and the gill lamellae of Astyanax aeneus (Pisces, Ostariophysi: Characidae) from the Rio Lacantún basin in the Biosphere Reserve of Montes Azules, Chiapas, Mexico. Parasitol Res, 112, 199–205

    Article  Google Scholar 

  16. 16.

    Mendoza-Franco, Reina and Torchin, 2009 Dactylogyrids (Monogenoidea) parasitizing the gills of Astyanax spp. (Characidae) from Panama and southeast Mexico, a new species of Diaphorocleidus and proposal for Characithecium n gen Journal of Parasitology, 95, 46–55

    Article  Google Scholar 

  17. 17.

    Mendoza-Garfias B, García-Prieto L, Pérez-Ponce De León. g. 2017 Checklist of the Monogenea (Platyhelminthes) parasitic in Mexican aquatic vertebrates Zoosystema, 39, 501–598 https://doi.org/10.5252/z2017n4a5

    Article  Google Scholar 

  18. 18.

    Mizelle J D, Kritsky D C, Crane J W 1968 Studies on monogenetic Trematodes. XXXVIII. Ancyrocephalinae from South America with the proposal of Jainus gen. n. Am Midl Nat, 80, 186–198

    Article  Google Scholar 

  19. 19.

    Moreira1 J., Scholz T., Luque J.L. 2016. A new species of Diaphorocleidus (Monogenea:Ancyrocephalinae) from the gills of Argonectes robertsi (Characiformes) and new records of dactylogyrids parasitic on fishes from the Xingu River, Amazon Basin, Brazil. Zoologia, 33, 1–6 https://doi.org/10.1590/S1984-4689zool-20160022

    Article  Google Scholar 

  20. 20.

    Santos N J F, Rodrigues A R O, Domingues M V 2015 Proposal of Whittingtonocotyle n gen (Dactylogyroidea: Dactylogyridae), with the description of two new species from the gills of Hoplerythrinus unitaeniatus (Characiformes: Erythrinidae) in Brazil Zootaxa, 1, 191–200

    Google Scholar 

  21. 21.

    Santos N J F, Costa N. G. S, Soares J B, Domingues M V 2018 Monogenoidean parasites of Acestrorhynchus falcatus (Characiformes: Acestrorhynchidae) from Pará, Brazil: species of Diaphorocleidus and Rhinoxenoides n gen (Monogenoidea: Dactylogyridae) Journal of Helminthology, 7, 1–12

    Google Scholar 

  22. 22.

    Rossin M A., Timi J T 2014 Characithecium (Monogenoidea: Dactylogyridae) parasitic on the Neotropical fish Oligosarcus jenynsii (Teleostei: Characidae) from the Pampasic region, Argentina, with the emendation of the genus. Zootaxa, 3, 382–396

    Article  Google Scholar 

  23. 23.

    Vidal-Martínez V M, Aguirre-Macedo ML, Scholz T, González-Solís D, Mendoza- Franco E F 2001 Atlas of the Helminth Parasites of Cichlid Fish of Mexico. Academia, Prague, Czech Republic. 165 pp

    Google Scholar 

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This study was supported by the project ‘‘Conservación, manejo y restauración de los ecosistemas acuáticos del Río Lacantún’’, promoted and coordinated by the Centro Interdisciplinario de Biodiversidad y Ambiente (CeIBA), A. C. with financing from Natura y Ecosistemas Mexicanos A. C. Permit for fish collections were issue by the Secretaría del Medio Ambiente y Recursos Naturales (SEMARNAT), Mexico (permit number: SGPA/DGVS/03492). We thank Delane C. Kritsky for helpful comments on an earlier version of this manuscript.

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Correspondence to Edgar F. Mendoza-Franco.

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Mendoza-Franco, E.F., Caspeta-Mandujano, J.M. & Ramírez-Martínez, C. Diaphorocleidus machacae n. sp. (Monogenea) Infecting the Gill Lamellae of Brycon guatemalensis (Characiformes: Bryconidae) from the Rio Lacantún Basin in Chiapas, Mexico. Acta Parasit. 64, 51–56 (2019). https://doi.org/10.2478/s11686-018-00007-7

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  • Diaphorocleidus
  • Dactylogyridae
  • Freshwater fish
  • Southeast Mexico
  • Central America
  • Biogeography