Acta Parasitologica

, Volume 64, Issue 1, pp 19–30 | Cite as

Simultaneous Occurrence of Borrelia miyamotoi, Borrelia burgdorferi Sensu Lato, Anaplasma phagocytophilum and Rickettsia helvetica in Ixodes ricinus Ticks in Urban Foci in Bratislava, Slovakia

  • Tatiana VaculováEmail author
  • Markéta Derdáková
  • Eva Špitalská
  • Radovan Václav
  • Michal Chvostáč
  • Veronika Rusňáková Tarageľová
Original Paper



Questing Ixodes ricinus ticks were collected in two urban parks (Železná studienka and Horský park) of the capital city of Slovakia, Bratislava, during two consecutive years in 2011 and 2012. A total of 932 ticks were analyzed for the presence of tick-borne agents: B. miyamotoi, B. burgdorferi s.l., A. phagocytophilum and R. helvetica.


PCR analysis confirmed the presence of all pathogens at both localities. The overall infection prevalence of B. miyamotoi, B. burgdorferi s.l., A. phagocytophilum and R. helvetica was 0.75, 13.2, 5.6 and 8.9%, respectively. B. burgdorferi s.l. positive samples were represented by six genospecies. The most frequent one was B. afzelii followed by B. garinii and B. valaisiana.


Our study confirms the presence of I. ricinus ticks and at least nine tick-borne bacterial agents in city forest parks, which are used for recreational purposes. Ordination analysis revealed significant differences in the composition of pathogens with respect to study site location, time of season and ambient temperature, despite the fact that both sites are located relatively close to one another within the city.


Ixodes ricinus Borrelia miyamotoi Borrelia burgdorferi sensu lato Anaplasma phagocytophilum Rickettsia helvetica Urban foci 



This study was financially supported from the Scientific Grant Agency of Ministry of Education and Slovak Academy of Sciences—mainly by the project VEGA Nos. 2/0119/17 (70%) and partially by VEGA 2/0068/17 and the Slovak Research and Development Agency under contract No. APVV-14-0274, No. APVV-16-0518 and No. APVV-14-0556.


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© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2019

Authors and Affiliations

  • Tatiana Vaculová
    • 1
    Email author
  • Markéta Derdáková
    • 1
  • Eva Špitalská
    • 2
  • Radovan Václav
    • 1
  • Michal Chvostáč
    • 1
  • Veronika Rusňáková Tarageľová
    • 1
  1. 1.Institute of Zoology, Slovak Academy of SciencesBratislavaSlovak Republic
  2. 2.Institute of Virology, Biomedical Research CenterSlovak Academy of SciencesBratislavaSlovak Republic

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