Seroprevalence of Neospora caninum infection in dairy cows in Northern provinces, Thailand
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Neospora caninum, an obligate intracellular protozoan parasite, is the causative agent of neosporosis, recognized as a major cause of bovine abortion around the world. Thailand is a developing agricultural country located in Southeast Asia. Livestock developments particularly in dairy cows of this country have been hampered by low productivity including milk and slow growth rate due to the impact of many pathogens including N. caninum. Currently, there is no effective method for control of neosporosis since there is less information regarding current status of infections. The objective of this study was to investigate the seroprevalence of neosporosis in dairy cows of the northern part of Thailand. During 2006–2007, the sera of 642 cows from 42 small farm holders with the top three highest consensus of dairy farms in the northern provinces, such as Chiang Rai, Chiang Mai and Lumpang were collected and performed tests. Antibodies to N. caninum were assayed by enzyme-linked immunosorbent assay (ELISA) with recombinant N. caninum surface antigen 1 (NcSAG1) and indirect fluorescent antibody test (IFAT). The overall prevalence of N. caninum infection in this study was 46.9% (301/642) by ELISA and 34.3% (220/642) by IFAT.
KeywordsNeospora caninum dairy cows NcSAG1 IFAT Thailand
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- Arunvipas P., Inpankaew T., Jittapalapong S. 2012a. Seroprevalence and Risk Factors of Neospora caninum Infection among Dairy Cows in the Western Provinces of Nakhon Pathom, Ratchaburi and Kanchanaburi, Thailand. Kasetsart Journal (Natural Science.), 46, 64–70.Google Scholar
- Chahan B., Gaturanga I., Xiaohong H., Liao M., Fukumoto S., Hirata H., Nishikawa Y., Suzuki H., Sugimoto C., Nagasawa H., Fujusaki K., Igarashi I., Mikami T., Xuan X. 2003. Serodiagnosis of Neospora caninum infection in cattle by enzyme-linked immunosorbent assay with recombinant truncated NcSAG1. Veterinary Parasitology, 118, 177–185.PubMedCrossRefGoogle Scholar
- Dubey J.P., Carpenter J.L., Speer A., Topper M.J., Uggla A. 1988. Newly recognized fatal protozoan disease of dogs. Journal of the American Veterinary Medical Association, 198, 1269–1285.Google Scholar
- Jenkins M.C., Caver J.A., Björkman C., Anderson T.C., Romand S., Vinyard B., Uggla A., Thulliez P., Dubey J.P. 2000. Serological investigation of an outbreak of Neospora caninum-associated abortion in a dairy herd in southeastern United States. Veterinary Parasitology, 94, 17–26.PubMedCrossRefGoogle Scholar
- Jittapalapong S., Sangvaranond A., Inpankaew T., Phasuk C., Pinyopanuwat N., Chimnoi W., Kengradomkij C., Saengow S., Pumhom P., Arunwipat P., Anakewit T., Robertson I.D. 2008. Seroprevalence of Neospora caninum infections of dairy cows in the North-east of Thailand. Kasetsart Journal (Natural Science), 42, 61–66.Google Scholar
- Nishikawa Y., Kousaka Y., Tragoolpua K., Xuan X., Makala L., Fujisaki K., Mikami T., Nagasawa H. 2001. Characterization of Neospora caninum surface protein NcSRS2 based on Baculovirus expression system and its application for serodiagnosis of Neospora infection. Journal of Clinical Microbiology, 39, 3987–3991.PubMedCentralPubMedCrossRefGoogle Scholar