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Acta Parasitologica

, Volume 59, Issue 2, pp 301–304 | Cite as

Confirming Hypoderma tarandi (Diptera: Oestridae) human ophthalmomyiasis by larval DNA barcoding

  • Bjørn Arne Rukke
  • Symira Cholidis
  • Arild Johnsen
  • Preben Ottesen
Original Paper

Abstract

DNA barcoding is a practical tool for species identification, when morphological classification of an organism is difficult. Herein we describe the utilisation of this technique in a case of ophthalmomyiasis interna. A 12-year-old boy was infested during a summer holiday in northern Norway, while visiting an area populated with reindeer. Following medical examination, a Diptera larva was surgically removed from the boy’s eye and tentatively identified from its morphological traits as Hypoderma tarandi (L.) (Diptera: Oestridae). Ultimately, DNA barcoding confirmed this impression. The larval cytochrome c oxidase subunit 1 (COI) DNA sequence was matched with both profiles of five adult H. tarandi from the same region where the boy was infested, and other established profiles of H. tarandi in the Barcode of Life Data Systems (BOLD) identification engine.

Keywords

Ophthalmomyiasis species identification DNA barcoding Hypoderma tarandi 

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Copyright information

© Versita Warsaw and Springer-Verlag Wien 2014

Authors and Affiliations

  • Bjørn Arne Rukke
    • 1
  • Symira Cholidis
    • 2
  • Arild Johnsen
    • 3
  • Preben Ottesen
    • 1
  1. 1.Department of Pest ControlNorwegian Institute of Public HealthOsloNorway
  2. 2.Department of OphthalmologyOslo University HospitalOsloNorway
  3. 3.Natural History MuseumUniversity of OsloOsloNorway

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